PMID- 33002680
OWN - NLM
STAT- MEDLINE
DCOM- 20210712
LR  - 20210712
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 250
DP  - 2020 Nov
TI  - Polyamine regulation of porcine reproductive and respiratory syndrome virus 
      infection depends on spermidine-spermine acetyltransferase 1.
PG  - 108839
LID - S0378-1135(20)30977-9 [pii]
LID - 10.1016/j.vetmic.2020.108839 [doi]
AB  - Like obligate intracellular parasites, viruses co-opt host cell resources to 
      establish productive infections. Polyamines are key aliphatic molecules that 
      perform important roles in cellular growth and proliferation. They are also 
      needed for the successful multiplication of various viruses. Little is known 
      about the effects of polyamines on Arteriviridae infections. Here, porcine 
      reproductive and respiratory syndrome virus (PRRSV), an economically prominent 
      porcine virus, was used to investigate virus-polyamine interactions. We found 
      that PRRSV infection significantly downregulated the levels of cellular 
      polyamines. Using an inhibitor or specific short interfering RNAs (siRNAs) of 
      ornithine decarboxylase 1, a key anabolic enzyme involved in the classical de 
      novo biosynthesis of polyamines, we found that polyamine depletion abrogated 
      PRRSV proliferation, and this effect was recoverable by adding exogenous 
      spermidine and spermine, but not putrescine to the cells, suggesting that the 
      host inhibits polyamine biosynthesis to restrict PRRSV proliferation. Further 
      analysis revealed that the expression level of spermidine-spermine 
      acetyltransferase 1 (SAT1), a catabolic enzyme that reduces spermidine and 
      spermine levels, was upregulated during PRRSV infection, but conversely, SAT1 had 
      an inhibitory effect on PRRSV reproduction. Our data show that polyamines are 
      important molecules during PRRSV-host interactions, and polyamines and their 
      biosynthetic pathways are potential therapeutic targets against PRRSV infection.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Zhou, Yanrong
AU  - Zhou Y
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory 
      of Preventive Veterinary Medicine in Hubei Province, the Cooperative Innovation 
      Center for Sustainable Pig Production, Wuhan 430070, China.
FAU - Hou, Zhenzhen
AU  - Hou Z
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory 
      of Preventive Veterinary Medicine in Hubei Province, the Cooperative Innovation 
      Center for Sustainable Pig Production, Wuhan 430070, China.
FAU - Fang, Liurong
AU  - Fang L
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory 
      of Preventive Veterinary Medicine in Hubei Province, the Cooperative Innovation 
      Center for Sustainable Pig Production, Wuhan 430070, China.
FAU - Ke, Qiyun
AU  - Ke Q
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory 
      of Preventive Veterinary Medicine in Hubei Province, the Cooperative Innovation 
      Center for Sustainable Pig Production, Wuhan 430070, China.
FAU - Xiong, Yujian
AU  - Xiong Y
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory 
      of Preventive Veterinary Medicine in Hubei Province, the Cooperative Innovation 
      Center for Sustainable Pig Production, Wuhan 430070, China.
FAU - Fang, Puxian
AU  - Fang P
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory 
      of Preventive Veterinary Medicine in Hubei Province, the Cooperative Innovation 
      Center for Sustainable Pig Production, Wuhan 430070, China.
FAU - Xiao, Shaobo
AU  - Xiao S
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory 
      of Preventive Veterinary Medicine in Hubei Province, the Cooperative Innovation 
      Center for Sustainable Pig Production, Wuhan 430070, China. Electronic address: 
      vet@mail.hzau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200919
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Polyamines)
RN  - 2FZ7Y3VOQX (Spermine)
RN  - EC 2.3.1.- (Acetyltransferases)
RN  - EC 2.3.1.57 (diamine N-acetyltransferase)
RN  - U87FK77H25 (Spermidine)
SB  - IM
MH  - Acetyltransferases/*genetics/metabolism
MH  - Animals
MH  - Cell Line
MH  - Down-Regulation
MH  - Gene Expression Regulation
MH  - *Host Microbial Interactions
MH  - Kidney/cytology
MH  - Polyamines/*metabolism
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Spermidine/*metabolism
MH  - Spermine/*metabolism
MH  - Swine
MH  - Up-Regulation
MH  - Virus Replication
PMC - PMC7501835
OTO - NOTNLM
OT  - Antiviral drug target
OT  - Polyamine
OT  - Porcine reproductive and respiratory syndrome virus
OT  - Spermidine-spermine acetyltransferase 1
COIS- The authors declare that there are no conflicts of interest.
EDAT- 2020/10/02 06:00
MHDA- 2021/07/13 06:00
PMCR- 2020/09/19
CRDT- 2020/10/01 20:13
PHST- 2020/06/03 00:00 [received]
PHST- 2020/08/31 00:00 [accepted]
PHST- 2020/10/02 06:00 [pubmed]
PHST- 2021/07/13 06:00 [medline]
PHST- 2020/10/01 20:13 [entrez]
PHST- 2020/09/19 00:00 [pmc-release]
AID - S0378-1135(20)30977-9 [pii]
AID - 108839 [pii]
AID - 10.1016/j.vetmic.2020.108839 [doi]
PST - ppublish
SO  - Vet Microbiol. 2020 Nov;250:108839. doi: 10.1016/j.vetmic.2020.108839. Epub 2020 
      Sep 19.