PMID- 33003582
OWN - NLM
STAT- MEDLINE
DCOM- 20210319
LR  - 20210319
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 9
IP  - 10
DP  - 2020 Sep 29
TI  - Interaction of Cytochrome C Oxidase with Steroid Hormones.
LID - 10.3390/cells9102211 [doi]
LID - 2211
AB  - Estradiol, testosterone and other steroid hormones inhibit cytochrome c oxidase 
      (CcO) purified from bovine heart. The inhibition is strongly dependent on 
      concentration of dodecyl-maltoside (DM) in the assay. The plots of K(i) vs [DM] 
      are linear for both estradiol and testosterone which may indicate an 1:1 
      stoichiometry competition between the hormones and the detergent. Binding of 
      estradiol, but not of testosterone, brings about spectral shift of the oxidized 
      CcO consistent with an effect on heme a(3)(3+). We presume that the hormones bind 
      to CcO at the bile acid binding site described by Ferguson-Miller and 
      collaborators. Estradiol is shown to inhibit intraprotein electron transfer 
      between hemes a and a(3)(.) Notably, neither estradiol nor testosterone 
      suppresses the peroxidase activity of CcO. Such a specific mode of action 
      indicates that inhibition of CcO activity by the hormones is associated with 
      impairing proton transfer via the K-proton channel.
FAU - Oleynikov, Ilya P
AU  - Oleynikov IP
AD  - A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow 
      State University, Leninskie gory 1, Bld. 40, 119 992 Moscow, Russia.
FAU - Azarkina, Natalia V
AU  - Azarkina NV
AD  - A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow 
      State University, Leninskie gory 1, Bld. 40, 119 992 Moscow, Russia.
FAU - Vygodina, Tatiana V
AU  - Vygodina TV
AD  - A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow 
      State University, Leninskie gory 1, Bld. 40, 119 992 Moscow, Russia.
FAU - Konstantinov, Alexander A
AU  - Konstantinov AA
AD  - A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow 
      State University, Leninskie gory 1, Bld. 40, 119 992 Moscow, Russia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200929
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
RN  - 0 (Cyanides)
RN  - 0 (Glucosides)
RN  - 0 (Gonadal Steroid Hormones)
RN  - 3XMK78S47O (Testosterone)
RN  - 42VZT0U6YR (Heme)
RN  - 4TI98Z838E (Estradiol)
RN  - 69227-93-6 (dodecyl maltoside)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cyanides/chemistry
MH  - Electron Transport
MH  - Electron Transport Complex IV/*antagonists & inhibitors/chemistry/*metabolism
MH  - Estradiol/metabolism
MH  - Glucosides/chemistry
MH  - Gonadal Steroid Hormones/*metabolism
MH  - Heme/*chemistry/metabolism
MH  - Kinetics
MH  - Oxidation-Reduction
MH  - Testosterone/metabolism
PMC - PMC7601700
OTO - NOTNLM
OT  - cytochrome oxidase
OT  - regulation
OT  - steroid hormones
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2020/10/03 06:00
MHDA- 2021/03/20 06:00
PMCR- 2020/10/01
CRDT- 2020/10/02 01:02
PHST- 2020/08/22 00:00 [received]
PHST- 2020/09/20 00:00 [revised]
PHST- 2020/09/23 00:00 [accepted]
PHST- 2020/10/02 01:02 [entrez]
PHST- 2020/10/03 06:00 [pubmed]
PHST- 2021/03/20 06:00 [medline]
PHST- 2020/10/01 00:00 [pmc-release]
AID - cells9102211 [pii]
AID - cells-09-02211 [pii]
AID - 10.3390/cells9102211 [doi]
PST - epublish
SO  - Cells. 2020 Sep 29;9(10):2211. doi: 10.3390/cells9102211.