PMID- 33007049
OWN - NLM
STAT- MEDLINE
DCOM- 20201231
LR  - 20240429
IS  - 1553-7374 (Electronic)
IS  - 1553-7366 (Print)
IS  - 1553-7366 (Linking)
VI  - 16
IP  - 10
DP  - 2020 Oct
TI  - Identification and characterisation of serotonin signalling in the potato cyst 
      nematode Globodera pallida reveals new targets for crop protection.
PG  - e1008884
LID - 10.1371/journal.ppat.1008884 [doi]
LID - e1008884
AB  - Plant parasitic nematodes are microscopic pathogens that invade plant roots and 
      cause extensive damage to crops. We have used a chemical biology approach to 
      define mechanisms underpinning their parasitic behaviour: We discovered that 
      reserpine, a plant alkaloid that inhibits the vesicular monoamine transporter 
      (VMAT), potently impairs the ability of the potato cyst nematode Globodera 
      pallida to enter the host plant root. We show this is due to an inhibition of 
      serotonergic signalling that is essential for activation of the stylet which is 
      used to access the host root. Prompted by this we identified core molecular 
      components of G. pallida serotonin signalling encompassing the target of 
      reserpine, VMAT; the synthetic enzyme for serotonin, tryptophan hydroxylase; the 
      G protein coupled receptor SER-7 and the serotonin-gated chloride channel MOD-1. 
      We cloned each of these molecular components and confirmed their functional 
      identity by complementation of the corresponding C. elegans mutant thus mapping 
      out serotonergic signalling in G. pallida. Complementary approaches testing the 
      effect of chemical inhibitors of each of these signalling elements on discrete 
      sub-behaviours required for parasitism and root invasion reinforce the critical 
      role of serotonin. Thus, targeting the serotonin signalling pathway presents a 
      promising new route to control plant parasitic nematodes.
FAU - Crisford, Anna
AU  - Crisford A
AUID- ORCID: 0000-0001-5775-643X
AD  - School of Biological Sciences, University of Southampton, Southampton, United 
      Kingdom.
FAU - Calahorro, Fernando
AU  - Calahorro F
AUID- ORCID: 0000-0003-0659-7728
AD  - School of Biological Sciences, University of Southampton, Southampton, United 
      Kingdom.
FAU - Ludlow, Elizabeth
AU  - Ludlow E
AD  - School of Biological Sciences, University of Southampton, Southampton, United 
      Kingdom.
FAU - Marvin, Jessica M C
AU  - Marvin JMC
AD  - Centre for Plant Sciences, School of Biology, Faculty of Biological Sciences, 
      University of Leeds, Leeds, United Kingdom.
FAU - Hibbard, Jennifer K
AU  - Hibbard JK
AD  - Centre for Plant Sciences, School of Biology, Faculty of Biological Sciences, 
      University of Leeds, Leeds, United Kingdom.
FAU - Lilley, Catherine J
AU  - Lilley CJ
AD  - Centre for Plant Sciences, School of Biology, Faculty of Biological Sciences, 
      University of Leeds, Leeds, United Kingdom.
FAU - Kearn, James
AU  - Kearn J
AD  - School of Biological Sciences, University of Southampton, Southampton, United 
      Kingdom.
FAU - Keefe, Francesca
AU  - Keefe F
AD  - School of Biological Sciences, University of Southampton, Southampton, United 
      Kingdom.
FAU - Johnson, Peter
AU  - Johnson P
AUID- ORCID: 0000-0001-7780-8911
AD  - School of Biological Sciences, University of Southampton, Southampton, United 
      Kingdom.
FAU - Harmer, Rachael
AU  - Harmer R
AD  - School of Biological Sciences, University of Southampton, Southampton, United 
      Kingdom.
FAU - Urwin, Peter E
AU  - Urwin PE
AUID- ORCID: 0000-0003-0398-2372
AD  - Centre for Plant Sciences, School of Biology, Faculty of Biological Sciences, 
      University of Leeds, Leeds, United Kingdom.
FAU - O'Connor, Vincent
AU  - O'Connor V
AD  - School of Biological Sciences, University of Southampton, Southampton, United 
      Kingdom.
FAU - Holden-Dye, Lindy
AU  - Holden-Dye L
AUID- ORCID: 0000-0002-9704-1217
AD  - School of Biological Sciences, University of Southampton, Southampton, United 
      Kingdom.
LA  - eng
GR  - P40 OD010440/OD/NIH HHS/United States
GR  - BB/J006017/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - BB/J006890/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20201002
PL  - United States
TA  - PLoS Pathog
JT  - PLoS pathogens
JID - 101238921
RN  - 333DO1RDJY (Serotonin)
SB  - IM
MH  - Animals
MH  - Crop Protection/*methods
MH  - *Host-Pathogen Interactions
MH  - Nematoda/*physiology
MH  - Plant Diseases/*parasitology
MH  - Serotonin/*metabolism
MH  - *Signal Transduction
MH  - Solanum tuberosum/*metabolism/parasitology
PMC - PMC7556481
COIS- I have read the journal's policy and the authors of this manuscript have the 
      following competing interests: Holden-Dye, O'Connor and Urwin are inventors for 
      filed UK Patent Application no. 1710057.9 Reserpine Seed Coating; United States 
      Patent Application No. 16/625,580 Reserpine Seed Coating; European Patent 
      Application No. 187337401.2-1110/3641543 Reserpine Seed Coating.
EDAT- 2020/10/03 06:00
MHDA- 2021/01/01 06:00
PMCR- 2020/10/02
CRDT- 2020/10/02 17:08
PHST- 2020/04/17 00:00 [received]
PHST- 2020/08/14 00:00 [accepted]
PHST- 2020/10/14 00:00 [revised]
PHST- 2020/10/03 06:00 [pubmed]
PHST- 2021/01/01 06:00 [medline]
PHST- 2020/10/02 17:08 [entrez]
PHST- 2020/10/02 00:00 [pmc-release]
AID - PPATHOGENS-D-20-00790 [pii]
AID - 10.1371/journal.ppat.1008884 [doi]
PST - epublish
SO  - PLoS Pathog. 2020 Oct 2;16(10):e1008884. doi: 10.1371/journal.ppat.1008884. 
      eCollection 2020 Oct.