PMID- 33010389
OWN - NLM
STAT- MEDLINE
DCOM- 20220221
LR  - 20220221
IS  - 1089-8646 (Electronic)
IS  - 0888-7543 (Linking)
VI  - 113
IP  - 1 Pt 2
DP  - 2021 Jan
TI  - Genome analysis of 1-deoxynojirimycin (1-DNJ)-producing Bacillus velezensis K26 
      and distribution of Bacillus sp. harboring a 1-DNJ biosynthetic gene cluster.
PG  - 647-653
LID - S0888-7543(20)30397-9 [pii]
LID - 10.1016/j.ygeno.2020.09.061 [doi]
AB  - 1-Deoxynojirumycin (1-DNJ) is a representative iminosugar with alpha-glucosidase 
      inhibition (AGI) activity. In this study, the full genome sequencing of 
      1-DNJ-producing Bacillus velezensis K26 was performed. The genome consists of a 
      circular chromosome (4,047,350 bps) with two types of putative virulence factors, 
      five antibiotic resistance genes, and seven secondary metabolite biosynthetic 
      gene clusters. Genomic analysis of a wide range of Bacillus species revealed that 
      a 1-DNJ biosynthetic gene cluster was commonly present in four Bacillus species 
      (B. velezensis, B. pseudomycoides, B. amyloliquefaciens, and B. atrophaeus). In 
      vitro experiments revealed that the increased mRNA expression levels of the three 
      1-DNJ biosynthetic genes were closely related to increased AGI activity. Genomic 
      comparison and alignment of multiple gene sequences indicated the conservation of 
      the 1-DNJ biosynthetic gene cluster in each Bacillus species. This genomic 
      analysis of Bacillus species having a 1-DNJ biosynthetic gene cluster could 
      provide a basis for further research on 1-DNJ-producing bacteria.
CI  - Copyright (c) 2020. Published by Elsevier Inc.
FAU - Lee, Hyunjin
AU  - Lee H
AD  - Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon 
      National University, Incheon 22012, Republic of Korea.
FAU - Jung, Dong-Hyun
AU  - Jung DH
AD  - Bacteria Research Team, Nakdonggang National Institute of Biological Resources, 
      Sangju 37242, Republic of Korea.
FAU - Seo, Dong-Ho
AU  - Seo DH
AD  - Department of Food Science and Technology, College of Agriculture and Life 
      Sciences, Jeonbuk National University, Jeonju 54896, Republic of Korea; 
      Department of Agricultural Convergence Technology, Jeonbuk National University, 
      Jeonju 54896, Republic of Korea.
FAU - Chung, Won-Hyong
AU  - Chung WH
AD  - Research Group of Healthcare, Korea Food Research Institute, Wanju 55365, 
      Republic of Korea. Electronic address: whchung@kfri.re.kr.
FAU - Seo, Myung-Ji
AU  - Seo MJ
AD  - Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon 
      National University, Incheon 22012, Republic of Korea; Division of 
      Bioengineering, Incheon National University, Incheon 22012, Republic of Korea. 
      Electronic address: mjseo@inu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201001
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (deoxynojirimycine)
RN  - 19130-96-2 (1-Deoxynojirimycin)
RN  - N08U5BOQ1K (Glucosamine)
RN  - Bacillus velezensis
SB  - IM
MH  - 1-Deoxynojirimycin
MH  - Bacillus/classification/*genetics/metabolism
MH  - *Genes, Bacterial
MH  - Glucosamine/*analogs & derivatives/biosynthesis/genetics
MH  - Multigene Family
MH  - Phylogeny
MH  - Sequence Homology
OTO - NOTNLM
OT  - 1-deoxynojirimycin
OT  - Bacillus velezensis
OT  - Genome
OT  - Isolation
OT  - alpha-Glucosidase inhibitor
EDAT- 2020/10/04 06:00
MHDA- 2022/02/22 06:00
CRDT- 2020/10/03 20:08
PHST- 2020/04/12 00:00 [received]
PHST- 2020/09/25 00:00 [revised]
PHST- 2020/09/29 00:00 [accepted]
PHST- 2020/10/04 06:00 [pubmed]
PHST- 2022/02/22 06:00 [medline]
PHST- 2020/10/03 20:08 [entrez]
AID - S0888-7543(20)30397-9 [pii]
AID - 10.1016/j.ygeno.2020.09.061 [doi]
PST - ppublish
SO  - Genomics. 2021 Jan;113(1 Pt 2):647-653. doi: 10.1016/j.ygeno.2020.09.061. Epub 
      2020 Oct 1.