PMID- 33011362
OWN - NLM
STAT- MEDLINE
DCOM- 20210617
LR  - 20210617
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 149
DP  - 2020 Dec
TI  - Molecular characterization of carp edema virus disease: An emerging threat to koi 
      Cyprinus carpio in China.
PG  - 104551
LID - S0882-4010(20)30917-7 [pii]
LID - 10.1016/j.micpath.2020.104551 [doi]
AB  - Carp edema virus disease (CEVD) has resulted in great economic losses in koi 
      (Cyprinus carpio koi) and common carp (Cyprinus carpio carpio) populations in the 
      world. In this study, the diseased koi were diagnosed as CEV infection based on 
      5' untranslated region (5'UTR) and 4a protein genes by the conventional PCR, 
      nested PCR and quantitative PCR (qPCR) analyses. Phylogenetic tree analysis 
      showed that the TJ201708 strain was classified into the genogroup IIa. 
      Furthermore, qPCR of 5'UTR gene revealed that the lowest detection limit was 
      4.0 fg/muL. The pathogenicity of CEV for koi was demonstrated in the infection 
      experiments. Histopathological examination revealed the petechial hemorrhages of 
      liver and spleen, vacuolization of lamina propria of intestine and swelling and 
      necrosis of respiratory epithelial cells of gills. To our knowledge, this is the 
      first report the qPCR of 5'UTR gene in the detection of carp edema virus.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Luo, Fuli
AU  - Luo F
AD  - Tianjin Key Lab of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin 
      Agricultural University, Tianjin, 300384, China.
FAU - Lian, Zhengyi
AU  - Lian Z
AD  - Tianjin Key Lab of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin 
      Agricultural University, Tianjin, 300384, China.
FAU - Niu, Yuchen
AU  - Niu Y
AD  - School of Chemical Engineering and Technology, Tianjin University, Tianjin, 
      300072, China.
FAU - Lu, Aijun
AU  - Lu A
AD  - Tianjin Key Lab of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin 
      Agricultural University, Tianjin, 300384, China. Electronic address: 
      lajand@126.com.
FAU - Hu, Xiucai
AU  - Hu X
AD  - Tianjin Key Lab of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin 
      Agricultural University, Tianjin, 300384, China.
FAU - Xie, Xinyan
AU  - Xie X
AD  - School of Chemical Engineering and Technology, Tianjin University, Tianjin, 
      300072, China.
FAU - Sun, Jingfeng
AU  - Sun J
AD  - Tianjin Key Lab of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin 
      Agricultural University, Tianjin, 300384, China.
LA  - eng
PT  - Journal Article
DEP - 20201001
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
SB  - IM
MH  - Animals
MH  - *Carps
MH  - China
MH  - Edema/veterinary
MH  - *Fish Diseases
MH  - Phylogeny
MH  - *Poxviridae Infections
OTO - NOTNLM
OT  - Carp edema virus
OT  - Histopathology
OT  - Koi
OT  - qPCR
EDAT- 2020/10/05 06:00
MHDA- 2021/06/22 06:00
CRDT- 2020/10/04 20:23
PHST- 2018/09/07 00:00 [received]
PHST- 2020/07/23 00:00 [revised]
PHST- 2020/09/28 00:00 [accepted]
PHST- 2020/10/05 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/10/04 20:23 [entrez]
AID - S0882-4010(20)30917-7 [pii]
AID - 10.1016/j.micpath.2020.104551 [doi]
PST - ppublish
SO  - Microb Pathog. 2020 Dec;149:104551. doi: 10.1016/j.micpath.2020.104551. Epub 2020 
      Oct 1.