PMID- 33013926
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 11
DP  - 2020
TI  - Dynamic Changes in the Phenotype of Dendritic Cells in the Uterus and Uterine 
      Draining Lymph Nodes After Coitus.
PG  - 557720
LID - 10.3389/fimmu.2020.557720 [doi]
LID - 557720
AB  - Dendritic cells (DCs) are essential for successful embryo implantation. However, 
      the properties of uterine DCs (uDCs) during the implantation period are not well 
      characterized. In this study, we investigated the dynamic changes in the uDC 
      phenotypes during the period between coitus and implantation. In virgin mice, we 
      evaluated the expressions of CD103 and XCR1, this is the first report to 
      demonstrate uDCs expressing CD103 in XCR1(+)cDC1s and XCR1(+)cDC2s. On day 0.5 
      post coitus (pc), the number of uterine CD11c(+)CD103(-)MHC 
      classII(high)CD86(high)-mature DCs rapidly increased and then decreased to 
      non-pregnancy levels on days 1.5 and 2.5 pc. On day 3.5 pc just before 
      implantation, the number of CD11c(+)CD103(+)MHC class II(dim)CD86(dim)-immature 
      DCs increased in the uterus. The increase in mature uDCs on day 1.5 pc was 
      observed in both allogeneic- and syngeneic mating, suggesting that sexual 
      intercourse, or semen, play a role in this process. Meanwhile, the increase in 
      immature uDCs on day 3.5 pc was only observed in allogeneic mating, suggesting 
      that allo-antigens in the semen contribute to this process. Next, to understand 
      the turnover and migration of uDCs, we monitored DC movement in the uterus and 
      uterine draining lymph nodes (dLNs) using photoconvertible protein Kikume Green 
      Red (KikGR) mice. On day 0.5 pc, uDCs were composed of equal numbers of remaining 
      DCs and migratory DCs. However, on day 3.5 pc, uDCs were primarily composed of 
      migratory DCs, suggesting that most of the uDCs migrate from the periphery just 
      before implantation. Finally, we studied the expression of PD-L2-which induces 
      immunoregulation-on DCs. On day 3.5 pc, PD-L2 was expressed on CD103(+)-mature 
      and CD103(-)-mature DCs in the uterus. However, PD-L2 expression on 
      CD103(-)-immature DCs and CD103(+)-immature DCs was very low. Furthermore, both 
      remaining and migratory DCs in the uterus and uterus-derived-DCs in the dLNs on 
      day 3.5 pc highly expressed PD-L2 on their surface. Therefore, our study findings 
      provide a better understanding of the dynamic changes occurring in uterine DCs 
      and dLNs in preparation for implantation following allogeneic- and syngeneic 
      mating.
CI  - Copyright (c) 2020 Yasuda, Shima, Moriya, Ikebuchi, Kusumoto, Ushijima, Nakashima, 
      Tomura and Saito.
FAU - Yasuda, Ippei
AU  - Yasuda I
AD  - Department of Obstetrics and Gynecology, University of Toyama, Toyama, Japan.
AD  - Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Osaka, 
      Japan.
FAU - Shima, Tomoko
AU  - Shima T
AD  - Department of Obstetrics and Gynecology, University of Toyama, Toyama, Japan.
FAU - Moriya, Taiki
AU  - Moriya T
AD  - Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Osaka, 
      Japan.
FAU - Ikebuchi, Ryoyo
AU  - Ikebuchi R
AD  - Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Osaka, 
      Japan.
AD  - Research Fellow of Japan Society for the Promotion of Science, Tokyo, Japan.
FAU - Kusumoto, Yutaka
AU  - Kusumoto Y
AD  - Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Osaka, 
      Japan.
FAU - Ushijima, Akemi
AU  - Ushijima A
AD  - Department of Obstetrics and Gynecology, University of Toyama, Toyama, Japan.
FAU - Nakashima, Akitoshi
AU  - Nakashima A
AD  - Department of Obstetrics and Gynecology, University of Toyama, Toyama, Japan.
FAU - Tomura, Michio
AU  - Tomura M
AD  - Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Osaka, 
      Japan.
FAU - Saito, Shigeru
AU  - Saito S
AD  - Department of Obstetrics and Gynecology, University of Toyama, Toyama, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200911
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Biomarkers)
SB  - IM
MH  - Animals
MH  - Biomarkers
MH  - Cell Differentiation/immunology
MH  - Cell Plasticity/immunology
MH  - Coitus/*physiology
MH  - Dendritic Cells/*immunology/*metabolism
MH  - Embryo Implantation/genetics/immunology
MH  - Female
MH  - Immunophenotyping
MH  - Lymph Nodes/*immunology/*metabolism
MH  - Mice
MH  - *Phenotype
MH  - Uterus/*physiology
PMC - PMC7516021
OTO - NOTNLM
OT  - Kikume Green Red (KikGR)
OT  - PD-L2
OT  - feto-maternal tolerance
OT  - photoconvertible protein
OT  - tolerogenic dendritic cells
OT  - uterus
EDAT- 2020/10/06 06:00
MHDA- 2021/05/15 06:00
PMCR- 2020/01/01
CRDT- 2020/10/05 06:16
PHST- 2020/04/30 00:00 [received]
PHST- 2020/08/20 00:00 [accepted]
PHST- 2020/10/05 06:16 [entrez]
PHST- 2020/10/06 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2020.557720 [doi]
PST - epublish
SO  - Front Immunol. 2020 Sep 11;11:557720. doi: 10.3389/fimmu.2020.557720. eCollection 
      2020.