PMID- 33014896
OWN - NLM
STAT- MEDLINE
DCOM- 20210617
LR  - 20220531
IS  - 2235-2988 (Electronic)
IS  - 2235-2988 (Linking)
VI  - 10
DP  - 2020
TI  - ERG-Associated lncRNA (ERGAL) Promotes the Stability and Integrity of Vascular 
      Endothelial Barrier During Dengue Viral Infection via Interaction With 
      miR-183-5p.
PG  - 477
LID - 10.3389/fcimb.2020.00477 [doi]
LID - 477
AB  - Dengue virus (DENV) continues to be a major public health problem. DENV infection 
      will cause mild dengue and severe dengue. Severe dengue is clinically manifested 
      as serious complications, including dengue hemorrhagic fever and/or dengue shock 
      syndrome (DHF/DSS), which is mainly characterized by vascular leakage. Currently, 
      the pathogenesis of severe dengue is not elucidated thoroughly, and there are no 
      known therapeutic targets for controlling the disease effectively. This study 
      aimed to further reveal the potential molecular mechanism of severe dengue. In 
      this study, the long non-coding RNA, ERG-associated lncRNA (lncRNA-ERGAL), was 
      activated and significantly up-regulated in DENV-infected vascular endothelial 
      cells. After knockdown of lncRNA-ERGAL, the expression of ERG, VE-cadherin, and 
      claudin-5 was repressed; besides, cell apoptosis was enhanced, and cytoskeletal 
      remodeling was disordered, leading to instability and increased permeability of 
      vascular endothelial barrier during DENV infection. Fluorescence in situ 
      hybridization (FISH) assay showed lncRNA-ERGAL to be mainly expressed in the 
      cytoplasm. Moreover, the expression of miR-183-5p was found to increase during 
      DENV infection and revealed to regulate ERG, junction-associated proteins, and 
      the cytoskeletal structure after overexpression and knockdown. Then, ERGAL was 
      confirmed to interact with miR-183-5p by luciferase reporter assay. Collectively, 
      ERGAL acted as a miRNA sponge that can promote stability and integrity of 
      vascular endothelial barrier during DENV infection via binding to miR-183-5p, 
      thus revealing the potential molecular mechanism of severe dengue and providing a 
      foundation for a promising clinical target in the future.
CI  - Copyright (c) 2020 Zheng, Wang, Cui, Guo, Si, Yan, Fang, Jiang, Jiang and Zhou.
FAU - Zheng, Baojia
AU  - Zheng B
AD  - Department of Microbiology and Immunology, School of Basic Medical Sciences, 
      Jinan University, Guangzhou, China.
FAU - Wang, Hui
AU  - Wang H
AD  - Department of Microbiology and Immunology, School of Basic Medical Sciences, 
      Jinan University, Guangzhou, China.
FAU - Cui, Guohui
AU  - Cui G
AD  - Key Laboratory of Tropical Disease Control, Ministry of Education, Sun Yat-sen 
      University, Guangzhou, China.
AD  - Department of Medical Microbiology, Zhongshan Medical College, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Guo, Qianfang
AU  - Guo Q
AD  - Key Laboratory of Tropical Disease Control, Ministry of Education, Sun Yat-sen 
      University, Guangzhou, China.
AD  - Department of Medical Microbiology, Zhongshan Medical College, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Si, Lulu
AU  - Si L
AD  - Key Laboratory of Tropical Disease Control, Ministry of Education, Sun Yat-sen 
      University, Guangzhou, China.
AD  - Department of Medical Microbiology, Zhongshan Medical College, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Yan, Huijun
AU  - Yan H
AD  - Key Laboratory of Tropical Disease Control, Ministry of Education, Sun Yat-sen 
      University, Guangzhou, China.
AD  - Department of Medical Microbiology, Zhongshan Medical College, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Fang, Danyun
AU  - Fang D
AD  - Key Laboratory of Tropical Disease Control, Ministry of Education, Sun Yat-sen 
      University, Guangzhou, China.
AD  - Department of Medical Microbiology, Zhongshan Medical College, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Jiang, Lifang
AU  - Jiang L
AD  - Key Laboratory of Tropical Disease Control, Ministry of Education, Sun Yat-sen 
      University, Guangzhou, China.
AD  - Department of Medical Microbiology, Zhongshan Medical College, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Jiang, Zhenyou
AU  - Jiang Z
AD  - Department of Microbiology and Immunology, School of Basic Medical Sciences, 
      Jinan University, Guangzhou, China.
FAU - Zhou, Junmei
AU  - Zhou J
AD  - Key Laboratory of Tropical Disease Control, Ministry of Education, Sun Yat-sen 
      University, Guangzhou, China.
AD  - Department of Medical Microbiology, Zhongshan Medical College, Sun Yat-sen 
      University, Guangzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200908
PL  - Switzerland
TA  - Front Cell Infect Microbiol
JT  - Frontiers in cellular and infection microbiology
JID - 101585359
RN  - 0 (ERG protein, human)
RN  - 0 (MIRN183 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (Transcriptional Regulator ERG)
SB  - IM
MH  - *Dengue
MH  - *Dengue Virus/genetics
MH  - Endothelial Cells
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *MicroRNAs/genetics
MH  - *RNA, Long Noncoding/genetics
MH  - Transcriptional Regulator ERG
MH  - *Virus Diseases
PMC - PMC7506072
OTO - NOTNLM
OT  - ERGAL
OT  - dengue virus
OT  - lncRNA
OT  - miRNA-microRNA
OT  - permeability
OT  - severe dengue infection
EDAT- 2020/10/06 06:00
MHDA- 2021/06/22 06:00
PMCR- 2020/01/01
CRDT- 2020/10/05 06:21
PHST- 2020/04/05 00:00 [received]
PHST- 2020/08/03 00:00 [accepted]
PHST- 2020/10/05 06:21 [entrez]
PHST- 2020/10/06 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fcimb.2020.00477 [doi]
PST - epublish
SO  - Front Cell Infect Microbiol. 2020 Sep 8;10:477. doi: 10.3389/fcimb.2020.00477. 
      eCollection 2020.