PMID- 33015781
OWN - NLM
STAT- MEDLINE
DCOM- 20210507
LR  - 20210507
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 24
IP  - 18
DP  - 2020 Sep
TI  - Effects of lncRNA-HEIH on proliferation, apoptosis and invasion of gastric cancer 
      cells.
PG  - 9400-9407
LID - 23023 [pii]
LID - 10.26355/eurrev_202009_23023 [doi]
AB  - OBJECTIVE: The aim of this study was to explore the expression of long non-coding 
      ribonucleic acid HEIH (lncRNA-HEIH) in gastric cancer (GC) tissues, and to 
      investigate its effects on the proliferation, apoptosis and invasion of HGC-27 
      cells. PATIENTS AND METHODS: A total of 80 tissue samples were collected from 
      patients diagnosed with GC in Shenzhen People's Hospital. Meanwhile, 
      para-carcinoma tissues were enrolled as normal controls (Control group). Total 
      RNA was extracted from tissues, and the expression of lncRNA-HEIH was detected 
      via quantitative reverse transcription-polymerase chain reaction (qRT-PCR). 
      HGC-27 cells were cultured and transfected with small-interfering RNA-HEIH 
      (si-HEIH group). At 48 h after transfection, cell proliferation, apoptosis and 
      invasion were detected via methyl thiazolyl tetrazolium (MTT) assay, terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay and 
      transwell assay, respectively. RESULTS: (1) Compared with Control group, the 
      expression of lncRNA-HEIH rose significantly in GC tissues (p<0.01). (2) The 
      expression of lncRNA-HEIH in HGC-27 cells was significantly down-regulated in 
      si-HEIH group compared with si-NC group (p<0.01). (3) Compared with those in 
      si-NC group, the proliferation of HGC-27 cells was suppressed (p<0.05), while the 
      apoptosis of HGC-27 cells was promoted (p<0.01) in si-HEIH group. (4) The 
      invasion of HGC-27 cells was remarkably inhibited in Si-HEIH group than si-NC 
      group (p<0.05). CONCLUSIONS: LncRNA-HEIH is highly expressed in GC patients, 
      which affects the proliferation, apoptosis and invasion of GC HGC-27 cells.
FAU - Yang, Y-J
AU  - Yang YJ
AD  - Department of Gastroenterology, The Second Clinical Medical College of Jinan 
      University, Shenzhen People's Hospital, Shenzhen, China. wangls168@163.com.
FAU - Luo, S
AU  - Luo S
FAU - Wang, L-S
AU  - Wang LS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - Aged
MH  - *Apoptosis
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Female
MH  - Humans
MH  - Male
MH  - RNA, Long Noncoding/genetics/*metabolism
MH  - Stomach Neoplasms/diagnosis/*metabolism/surgery
EDAT- 2020/10/06 06:00
MHDA- 2021/05/08 06:00
CRDT- 2020/10/05 06:25
PHST- 2020/10/05 06:25 [entrez]
PHST- 2020/10/06 06:00 [pubmed]
PHST- 2021/05/08 06:00 [medline]
AID - 23023 [pii]
AID - 10.26355/eurrev_202009_23023 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2020 Sep;24(18):9400-9407. doi: 
      10.26355/eurrev_202009_23023.