PMID- 33015806
OWN - NLM
STAT- MEDLINE
DCOM- 20210507
LR  - 20210507
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 24
IP  - 18
DP  - 2020 Sep
TI  - MiR-31 aggravates inflammation and apoptosis in COPD rats via activating the 
      NF-kappaB signaling pathway.
PG  - 9626-9632
LID - 23051 [pii]
LID - 10.26355/eurrev_202009_23051 [doi]
AB  - OBJECTIVE: To study the effect of micro ribonucleic acid (miR)-31 on rats with 
      chronic obstructive pulmonary disease (COPD) by activating the nuclear factor-kappaB 
      (NF-kappaB) signaling pathway. MATERIALS AND METHODS: A total of 36 Sprague-Dawley 
      rats were randomly divided into normal group (n=12), model group (n=12) and 
      miR-31 mimics group (n=12). The rats were fed normally in normal group. In model 
      group, the COPD model was first established, followed by intervention using 
      normal saline. In miR-31 mimics group, the COPD model was also first established, 
      followed by intervention using miR-31 mimics. The expression of NF-kappaB was 
      detected via immunohistochemistry. Protein expressions of B-cell lymphoma-2 
      (Bcl-2) and Bcl-2 associated X protein (Bax) were determined through Western 
      blotting. Serum levels of interleukin-6 (IL-6), IL-18 and tumor necrosis factor-alpha 
      (TNF-alpha) were measured via enzyme-linked immunosorbent assay (ELISA). Moreover, 
      the apoptosis was examined via terminal deoxynucleotidyl transferase-mediated 
      dUTP nick end labeling (TUNEL) assay, and the relative expression of miR-31 was 
      detected by means of quantitative polymerase chain reaction (qPCR). RESULTS: The 
      immunohistochemistry results showed that the positive expression of NF-kappaB was 
      significantly higher in the other two groups than that in normal group (p<0.05), 
      while it was also remarkably higher in miR-31 mimics group than that in model 
      group (p<0.05). The results of Western blotting revealed that the relative 
      protein expression of Bax significantly increased, while that of Bcl-2 notably 
      declined in the other two groups compared with those in normal group (p<0.05). 
      Similarly, the relative protein expression of Bax was upregulated, while that of 
      Bcl-2 was distinctly reduced in miR-31 mimics group compared with those in model 
      group (p<0.05). It was found via ELISA that the model group and miR-31 mimics 
      group had evidently higher levels of IL-6, IL-18 and TNF-alpha than those in normal 
      group (p<0.05), while miR-31 mimics group also had prominently higher levels than 
      those in model group (p<0.05). In addition, according to the TUNEL assay, the 
      apoptosis rate remarkably increased in the other two groups in comparison with 
      that in normal group (p<0.05), while it remarkably rose in miR-31 mimics group 
      compared with that in model group (p<0.05). Finally, a significantly higher 
      expression of miR-31 was observed in the other two groups than that in normal 
      group via qPCR (p<0.05), and such a higher expression was also found in miR-31 
      mimics group than that in model group (p<0.05). CONCLUSIONS: MiR-31 aggravates 
      inflammation and apoptosis in COPD rats by activating the NF-kappaB signaling 
      pathway.
FAU - Wu, H
AU  - Wu H
AD  - Department of Respiratory Medicine, Shaanxi Provincial People's Hospital, Xian, 
      China. shanxiliwu@126.com.
FAU - Miao, Y
AU  - Miao Y
FAU - Shang, L-Q
AU  - Shang LQ
FAU - Chen, R-L
AU  - Chen RL
FAU - Yang, S-M
AU  - Yang SM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (MIRN31 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (NF-kappa B)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Disease Models, Animal
MH  - Inflammation/*metabolism/pathology
MH  - MicroRNAs/*metabolism
MH  - NF-kappa B/*metabolism
MH  - Pulmonary Disease, Chronic Obstructive/*metabolism/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction
EDAT- 2020/10/06 06:00
MHDA- 2021/05/08 06:00
CRDT- 2020/10/05 06:25
PHST- 2020/10/05 06:25 [entrez]
PHST- 2020/10/06 06:00 [pubmed]
PHST- 2021/05/08 06:00 [medline]
AID - 23051 [pii]
AID - 10.26355/eurrev_202009_23051 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2020 Sep;24(18):9626-9632. doi: 
      10.26355/eurrev_202009_23051.