PMID- 33016024
OWN - NLM
STAT- MEDLINE
DCOM- 20201102
LR  - 20201102
IS  - 1976-555X (Electronic)
IS  - 1229-845X (Print)
IS  - 1229-845X (Linking)
VI  - 21
IP  - 5
DP  - 2020 Sep
TI  - Development of a biosensor from aptamers for detection of the porcine 
      reproductive and respiratory syndrome virus.
PG  - e79
LID - 10.4142/jvs.2020.21.e79 [doi]
LID - e79
AB  - BACKGROUND: Recently, the pork industry of Thailand faced an epidemic of highly 
      virulent strains of porcine reproductive and respiratory syndrome virus (PRRSV), 
      which spread throughout Southeast Asia, including the Lao People's Democratic 
      Republic and Cambodia. Hence, the rapid and on-site screening of infected pigs on 
      a farm is essential. OBJECTIVES: To develop the new aptamer as a biosensor for 
      detection PRRSV which are rapid and on-site screening of infected pig. METHODS: 
      New aptamers against PRSSV were identified using the combined techniques of 
      capillary electrophoresis, colorimetric assay by gold nanoparticles, and quartz 
      crystal microbalance (QCM). RESULTS: Thirty-six candidate aptamers of the PRRSV 
      were identified from the systematic evolution of ligands by exponential 
      enrichment (SELEX) by capillary electrophoresis. Only 8 out of 36 aptamers could 
      bind to the PRSSV, as shown in a colorimetric assay. Of the 8 aptamers tested, 
      only the 1F aptamer could bind specifically to the PRSSV when presented with the 
      classical swine fever virus and a pseudo rabies virus. The QCM was used to 
      confirm the specificity and sensitivity of the 1F aptamer with a detection limit 
      of 1.87 x 10(10) particles. CONCLUSIONS: SELEX screening of the aptamer equipped 
      with capillary electrophoresis potentially revealed promising candidates for 
      detecting the PRRSV. The 1F aptamer exhibited the highest specificity and 
      selectivity against the PRRSV. These findings suggest that 1F is a promising 
      aptamer for further developing a novel PRRSV rapid detection kit.
CI  - (c) 2020 The Korean Society of Veterinary Science.
FAU - Kuitio, Chakpetch
AU  - Kuitio C
AUID- ORCID: 0000-0002-6005-8023
AD  - Genetic Engineering Interdisciplinary Program, Graduate School, Kasetsart 
      University, Bangkok 10900, Thailand.
FAU - Rasri, Natchaya
AU  - Rasri N
AUID- ORCID: 0000-0002-7122-7558
AD  - Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 
      10900, Thailand.
FAU - Kiriwan, Duangnapa
AU  - Kiriwan D
AUID- ORCID: 0000-0003-4824-7398
AD  - Genetic Engineering Interdisciplinary Program, Graduate School, Kasetsart 
      University, Bangkok 10900, Thailand.
FAU - Unajak, Sasimanas
AU  - Unajak S
AUID- ORCID: 0000-0002-3242-3978
AD  - Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 
      10900, Thailand.
FAU - Choowongkomon, Kiattawee
AU  - Choowongkomon K
AUID- ORCID: 0000-0002-2421-7859
AD  - Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 
      10900, Thailand.
AD  - Center for Advanced Studies in Nanotechnology for Chemical, Food and Agricultural 
      Industries, KU Institute for Advanced Studies, Kasetsart University, Bangkok 
      10900, Thailand. fsciktc@ku.ac.th.
LA  - eng
GR  - PHD59I0001/NRCT/National Research Council of Thailand/Thailand
GR  - KU/Kasetsart University/Thailand
PT  - Journal Article
PL  - Korea (South)
TA  - J Vet Sci
JT  - Journal of veterinary science
JID - 100964185
RN  - 0 (Aptamers, Nucleotide)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Animals
MH  - Aptamers, Nucleotide/*chemistry
MH  - Biosensing Techniques/methods/*veterinary
MH  - Colorimetry/veterinary
MH  - Gold/chemistry
MH  - Metal Nanoparticles/chemistry
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis/virology
MH  - Porcine respiratory and reproductive syndrome virus/*isolation & purification
MH  - Quartz Crystal Microbalance Techniques/veterinary
MH  - SELEX Aptamer Technique/methods/*veterinary
MH  - Sensitivity and Specificity
MH  - Swine
PMC - PMC7533388
OTO - NOTNLM
OT  - Porcine reproductive and respiratory syndrome virus
OT  - QCM
OT  - aptamer
OT  - detection
OT  - gold nanoparticles
OT  - l biosensor
COIS- The authors declare no conflicts of interest.
EDAT- 2020/10/06 06:00
MHDA- 2020/11/03 06:00
PMCR- 2020/09/01
CRDT- 2020/10/05 06:28
PHST- 2020/04/28 00:00 [received]
PHST- 2020/08/11 00:00 [revised]
PHST- 2020/08/18 00:00 [accepted]
PHST- 2020/10/05 06:28 [entrez]
PHST- 2020/10/06 06:00 [pubmed]
PHST- 2020/11/03 06:00 [medline]
PHST- 2020/09/01 00:00 [pmc-release]
AID - 21.e79 [pii]
AID - 10.4142/jvs.2020.21.e79 [doi]
PST - ppublish
SO  - J Vet Sci. 2020 Sep;21(5):e79. doi: 10.4142/jvs.2020.21.e79.