PMID- 33020132
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20210621
IS  - 2052-4897 (Electronic)
IS  - 2052-4897 (Linking)
VI  - 8
IP  - 1
DP  - 2020 Oct
TI  - Effect of bariatric surgery on circulating and urinary mitochondrial DNA copy 
      numbers in obesity with or without diabetes.
LID - 10.1136/bmjdrc-2020-001372 [doi]
LID - e001372
AB  - INTRODUCTION: Recent studies have suggested that extracellular circulating and 
      urinary mitochondrial DNA (mtDNA) are associated with mitochondrial dysfunction 
      in obesity and type 2 diabetes mellitus (T2DM). However, the changes to cell-free 
      serum and urinary mtDNA after bariatric surgery in patients with obesity with 
      T2DM have not been investigated to date. RESEARCH DESIGN AND METHODS: We 
      prospectively recruited patients with obesity (n=18), and with obesity and T2DM 
      (n=14) who underwent bariatric surgery, along with healthy volunteers (HV) as a 
      control group (n=22). Serum and urinary mitochondrial nicotinamide adenine 
      dinucleotide dehydrogenase subunit-1 (mtND-1) and cytochrome-c oxidase 3 
      (mtCOX-3) copy numbers were measured using quantitative PCR (qPCR). The mtDNA 
      copy numbers of patients with obesity (with and without T2DM) were followed up 6 
      months after surgery. RESULTS: The copy numbers of urinary mtND-1 and mtCOX-3 in 
      patients with obesity, with or without T2DM, were higher than those in the HVs. 
      Moreover, urinary mtCOX-3 copy number increased in patients with obesity with 
      T2DM compared with patients with obesity without T2DM (p=0.018). Meanwhile, serum 
      mtCOX-3 copy numbers in HV were higher in both obesity patient groups (p=0.040). 
      Bariatric surgery reduced urinary mtND-1 and mtCOX-3 copy numbers, as well as 
      serum mtCOX-3 copy numbers only in patients with obesity with T2DM. CONCLUSION: 
      These results suggest that T2DM induces greater kidney mitochondrial dysfunction 
      in patients with obesity, which can be effectively restored with bariatric 
      surgery.
CI  - (c) Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No 
      commercial re-use. See rights and permissions. Published by BMJ.
FAU - Seo, Mihae
AU  - Seo M
AD  - Internal Medicine, Soonchunhyang University Hospital, Gumi, Gyeongsangbuk-do, The 
      Republic of Korea.
FAU - Kim, Hyoungnae
AU  - Kim H
AUID- ORCID: 0000-0002-5359-0214
AD  - Internal Medicine, Soonchunhyang University Seoul Hospital, Seoul, The Republic 
      of Korea.
AD  - Hyaonam Kidney Laboratory, Soonchunhyang University Seoul Hospital, Seoul, The 
      Republic of Korea.
FAU - Noh, Hyunjin
AU  - Noh H
AD  - Internal Medicine, Soonchunhyang University Seoul Hospital, Seoul, The Republic 
      of Korea.
AD  - Hyaonam Kidney Laboratory, Soonchunhyang University Seoul Hospital, Seoul, The 
      Republic of Korea.
FAU - Jeon, Jin Seok
AU  - Jeon JS
AD  - Internal Medicine, Soonchunhyang University Seoul Hospital, Seoul, The Republic 
      of Korea.
AD  - Hyaonam Kidney Laboratory, Soonchunhyang University Seoul Hospital, Seoul, The 
      Republic of Korea.
FAU - Byun, Dong Won
AU  - Byun DW
AD  - Internal Medicine, Soonchunhyang University Seoul Hospital, Seoul, The Republic 
      of Korea.
FAU - Kim, Sang Hyun
AU  - Kim SH
AD  - Department of Surgery, Soonchunhyang University Seoul Hospital, Seoul, The 
      Republic of Korea.
FAU - Kim, Hye Jeong
AU  - Kim HJ
AD  - Internal Medicine, Soonchunhyang University Seoul Hospital, Seoul, The Republic 
      of Korea.
FAU - Suh, Kyoil
AU  - Suh K
AD  - Internal Medicine, Soonchunhyang University Seoul Hospital, Seoul, The Republic 
      of Korea.
FAU - Park, Hyeong Kyu
AU  - Park HK
AD  - Internal Medicine, Soonchunhyang University Seoul Hospital, Seoul, The Republic 
      of Korea ksoonhyo@schmc.ac.kr hkpark@schmc.ac.kr.
FAU - Kwon, Soon Hyo
AU  - Kwon SH
AUID- ORCID: 0000-0002-4114-4196
AD  - Internal Medicine, Soonchunhyang University Seoul Hospital, Seoul, The Republic 
      of Korea ksoonhyo@schmc.ac.kr hkpark@schmc.ac.kr.
AD  - Hyaonam Kidney Laboratory, Soonchunhyang University Seoul Hospital, Seoul, The 
      Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - BMJ Open Diabetes Res Care
JT  - BMJ open diabetes research & care
JID - 101641391
RN  - 0 (DNA, Mitochondrial)
SB  - IM
MH  - *Bariatric Surgery
MH  - DNA Copy Number Variations
MH  - DNA, Mitochondrial/genetics
MH  - *Diabetes Mellitus, Type 2/complications/genetics
MH  - Humans
MH  - Mitochondria
MH  - Obesity/complications/genetics/surgery
PMC - PMC7536782
OTO - NOTNLM
OT  - DNA
OT  - bariatric surgery
OT  - complementary
OT  - diabetes mellitus
OT  - obesity
OT  - type 2
COIS- Competing interests: None declared.
EDAT- 2020/10/07 06:00
MHDA- 2021/06/22 06:00
PMCR- 2020/10/05
CRDT- 2020/10/06 05:26
PHST- 2020/03/19 00:00 [received]
PHST- 2020/07/22 00:00 [revised]
PHST- 2020/08/23 00:00 [accepted]
PHST- 2020/10/06 05:26 [entrez]
PHST- 2020/10/07 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/10/05 00:00 [pmc-release]
AID - 8/1/e001372 [pii]
AID - bmjdrc-2020-001372 [pii]
AID - 10.1136/bmjdrc-2020-001372 [doi]
PST - ppublish
SO  - BMJ Open Diabetes Res Care. 2020 Oct;8(1):e001372. doi: 
      10.1136/bmjdrc-2020-001372.