PMID- 33020563
OWN - NLM
STAT- MEDLINE
DCOM- 20210122
LR  - 20211005
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Oct 5
TI  - The immune receptor CD300e negatively regulates T cell activation by impairing 
      the STAT1-dependent antigen presentation.
PG  - 16501
LID - 10.1038/s41598-020-73552-9 [doi]
LID - 16501
AB  - CD300e is a surface receptor, expressed by myeloid cells, involved in the tuning 
      of immune responses. CD300e engagement was reported to provide the cells with 
      survival signals, to trigger the expression of activation markers and the release 
      of pro-inflammatory cytokines. Hence, CD300e is considered an immune activating 
      receptor. In this study, we demonstrate that the ligation of CD300e in monocytes 
      hampers the expression of the human leukocyte antigen (HLA) class II, affecting 
      its synthesis. This effect, which is associated with the transcription impairment 
      of the signal transducer and activator of transcription 1 (STAT1), overcomes the 
      capacity of interferon gamma (IFN-gamma) to promote the expression of the 
      antigen-presenting molecules. Importantly, the decreased expression of HLA-II on 
      the surface of CD300e-activated monocytes negatively impacts their capacity to 
      activate T cells in an antigen-specific manner. Notably, unlike in vitro- 
      differentiated macrophages which do not express CD300e, the immune receptor is 
      expressed by tissue macrophages. Taken together, our findings argue against the 
      possibility that this molecule should be considered an activating immune receptor 
      sensu stricto. Moreover, our results support the notion that CD300e might be a 
      new player in the regulation of the expansion of T cell-mediated responses.
FAU - Coletta, Sara
AU  - Coletta S
AD  - Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35131, Padova, 
      Italy.
FAU - Salvi, Valentina
AU  - Salvi V
AD  - Department of Molecular and Translational Medicine, University of Brescia, 
      Brescia, Italy.
FAU - Della Bella, Chiara
AU  - Della Bella C
AD  - Department of Experimental and Clinical Medicine, University of Florence, 
      Florence, Italy.
FAU - Bertocco, Ambra
AU  - Bertocco A
AD  - Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35131, Padova, 
      Italy.
FAU - Lonardi, Silvia
AU  - Lonardi S
AD  - Department of Molecular and Translational Medicine, Section of Pathology, 
      University of Brescia, Brescia, Italy.
FAU - Trevellin, Elisabetta
AU  - Trevellin E
AD  - Department of Medicine, Clinica Medica 3a, Azienda Ospedaliera Di Padova, 
      University of Padova, Padova, Italy.
FAU - Fassan, Matteo
AU  - Fassan M
AD  - Department of Medicine, Clinica Medica 3a, Azienda Ospedaliera Di Padova, 
      University of Padova, Padova, Italy.
FAU - D'Elios, Mario M
AU  - D'Elios MM
AD  - Department of Experimental and Clinical Medicine, University of Florence, 
      Florence, Italy.
FAU - Vermi, William
AU  - Vermi W
AD  - Department of Molecular and Translational Medicine, Section of Pathology, 
      University of Brescia, Brescia, Italy.
FAU - Vettor, Roberto
AU  - Vettor R
AD  - Department of Medicine, Clinica Medica 3a, Azienda Ospedaliera Di Padova, 
      University of Padova, Padova, Italy.
FAU - Cagnin, Stefano
AU  - Cagnin S
AD  - Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35131, Padova, 
      Italy.
AD  - CRIBI Biotechnology Center, University of Padova, Padova, Italy.
FAU - Sozzani, Silvano
AU  - Sozzani S
AD  - Laboratory Affiliated To Istituto Pasteur Italia-Fondazione Cenci Bolognetti, 
      Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy.
FAU - Codolo, Gaia
AU  - Codolo G
AD  - Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35131, Padova, 
      Italy. gaia.codolo@unipd.it.
FAU - de Bernard, Marina
AU  - de Bernard M
AD  - Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35131, Padova, 
      Italy. marina.debernard@unipd.it.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201005
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (CD300E protein, human)
RN  - 0 (Cytokines)
RN  - 0 (HLA Antigens)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (STAT1 Transcription Factor)
RN  - 0 (STAT1 protein, human)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Antigen Presentation/immunology
MH  - Cell Line
MH  - Cytokines/metabolism
MH  - HLA Antigens/metabolism
MH  - Healthy Volunteers
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Lymphocyte Activation/*immunology
MH  - Monocytes/metabolism
MH  - Receptors, Immunologic/*metabolism
MH  - STAT1 Transcription Factor/*metabolism
MH  - Signal Transduction
MH  - T-Lymphocytes/immunology
PMC - PMC7536427
COIS- The authors declare no competing interests.
EDAT- 2020/10/07 06:00
MHDA- 2021/01/23 06:00
PMCR- 2020/10/05
CRDT- 2020/10/06 05:29
PHST- 2020/05/19 00:00 [received]
PHST- 2020/09/17 00:00 [accepted]
PHST- 2020/10/06 05:29 [entrez]
PHST- 2020/10/07 06:00 [pubmed]
PHST- 2021/01/23 06:00 [medline]
PHST- 2020/10/05 00:00 [pmc-release]
AID - 10.1038/s41598-020-73552-9 [pii]
AID - 73552 [pii]
AID - 10.1038/s41598-020-73552-9 [doi]
PST - epublish
SO  - Sci Rep. 2020 Oct 5;10(1):16501. doi: 10.1038/s41598-020-73552-9.