PMID- 33023362
OWN - NLM
STAT- MEDLINE
DCOM- 20211011
LR  - 20211011
IS  - 1744-8387 (Electronic)
IS  - 1478-9450 (Linking)
VI  - 17
IP  - 9
DP  - 2020 Sep
TI  - Differential expression of MAP3K7 and TROPONIN C proteins and related 
      perturbations in renal amyloidosis.
PG  - 685-694
LID - 10.1080/14789450.2020.1833722 [doi]
AB  - OBJECTIVES: Renal amyloidosis (RA) is a rare protein misfolding disorder that 
      prompts progressive renal insufficiency. This study aimed to decipher proteomic 
      changes in human sera to understand the pathophysiology and molecular mechanisms 
      underlying the disease development, hence assisting in the diagnosis of RA. 
      METHODS: Serum proteomic analysis was performed using a gel-based approach 
      followed by MALDI-TOF MS. RA patients with age and sex matched healthy volunteers 
      were recruited from Max Super Speciality Hospital, New Delhi, India. RESULTS: 
      Proteome profiles of serum revealed eight differentially expressed proteins 
      namely, Zinc finger protein 624, Protein FAM183A, Calcium-binding mitochondrial 
      carrier protein Scamc-3, V-type proton ATPase 116 kDa subunit A isoforms 2, 
      Protein TXNRD3NB, ATP - dependent RNA helicase, Troponin C and Mitogen-activated 
      protein kinase kinase kinase 7. These proteins were reported first time in RA. 
      The increased levels of MAP3K7 and TROPONIN C were validated by bio-layer 
      interferometry and their diagnostic accuracy was evaluated by ROC curve analysis. 
      The differentially expressed proteins were predominantly associated with 
      vesicular trafficking, transcriptional regulation, metabolic processes, apoptotic 
      process and mitochondrial metabolism. CONCLUSION: The results indicate that these 
      proteomic signatures may be considered as potential molecular targets for RA 
      diagnostics and therapeutics subject to validation on large sample size. 
      Abbreviations: AbetaP= Amyloid-beta protein, Abeta=Amyloid-beta, AL= Light chain 
      amyloidosis, AA= Amyloid A, ALECT2= LECT2 amyloidosis, APS= Ammonium persulfate 
      CKD= Chronic Kidney Diseases, EBRT= external beam radiation therapy, ESRD= 
      End-Stage Kidney Disease, Glis2= Gli-similar 2, JNK= c-Jun NH 2-terminal kinase, 
      MAPK= Mitogen-Activated Protein Kinase, MM=Multiple Myeloma, PHD= Prolyl 
      hydroxylase, RA = Renal Amyloidosis, SAA= Serum Amyloid A, SD= Standard 
      Deviation, Sepp= Selenoprotein, SCC= Squamous cell carcinoma, SDS= Sodium dodecyl 
      sulfate, TEMED = tetramethyl ethylenediamine, TGF-Beta-1=Transforming growth 
      factor- Beta-1, Trx = Thioredoxin, TrxR= Thioredoxin reductase.
FAU - Gupta, Nimisha
AU  - Gupta N
AD  - Department of Biotechnology, School of Chemical and Life Sciences, Jamia Hamdard 
      , New Delhi, India.
FAU - Sahar, Tahreem
AU  - Sahar T
AD  - Department of Biotechnology, School of Chemical and Life Sciences, Jamia Hamdard 
      , New Delhi, India.
FAU - Khullar, Dinesh
AU  - Khullar D
AD  - Nephrology and Renal Transplant Medicine, Max Super Speciality Hospital , New 
      Delhi, India.
FAU - Jain, S K
AU  - Jain SK
AD  - Department of Biochemistry, Hamdard Institute of Medical Sciences and Research, 
      Jamia Hamdard , New Delhi, India.
FAU - Wajid, Saima
AU  - Wajid S
AUID- ORCID: 0000-0003-1558-8233
AD  - Department of Biotechnology, School of Chemical and Life Sciences, Jamia Hamdard 
      , New Delhi, India.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201101
PL  - England
TA  - Expert Rev Proteomics
JT  - Expert review of proteomics
JID - 101223548
RN  - 0 (FAM183A protein, human)
RN  - 0 (Membrane Proteins)
RN  - 0 (Troponin C)
RN  - EC 2.7.11.25 (MAP Kinase Kinase Kinases)
RN  - EC 2.7.11.25 (MAP kinase kinase kinase 7)
SB  - IM
MH  - Amyloidosis/*blood
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Humans
MH  - Interferometry
MH  - Kidney Diseases/*blood
MH  - MAP Kinase Kinase Kinases/*blood
MH  - Membrane Proteins/blood
MH  - Mitochondria/metabolism
MH  - Proteomics/methods
MH  - Troponin C/*blood
OTO - NOTNLM
OT  - MALDI-TOF MS
OT  - Proteomics
OT  - bio-layer Interferometry
OT  - renal amyloidosis
OT  - two-dimensional electrophoresis
EDAT- 2020/10/08 06:00
MHDA- 2021/10/12 06:00
CRDT- 2020/10/07 05:31
PHST- 2020/10/08 06:00 [pubmed]
PHST- 2021/10/12 06:00 [medline]
PHST- 2020/10/07 05:31 [entrez]
AID - 10.1080/14789450.2020.1833722 [doi]
PST - ppublish
SO  - Expert Rev Proteomics. 2020 Sep;17(9):685-694. doi: 
      10.1080/14789450.2020.1833722. Epub 2020 Nov 1.