PMID- 33023470
OWN - NLM
STAT- MEDLINE
DCOM- 20210927
LR  - 20210927
IS  - 1471-213X (Electronic)
IS  - 1471-213X (Linking)
VI  - 20
IP  - 1
DP  - 2020 Oct 6
TI  - Monocyte Chemoattractant Protein-1 stimulates the differentiation of rat stem and 
      progenitor Leydig cells during regeneration.
PG  - 20
LID - 10.1186/s12861-020-00225-1 [doi]
LID - 20
AB  - BACKGROUND: Monocyte chemoattractant protein-1(MCP-1) is a chemokine secreted by 
      Leydig cells and peritubular myoid cells in the rat testis. Its role in 
      regulating the development of Leydig cells via autocrine and paracrine is still 
      unclear. The objective of the current study was to investigate the effects of 
      MCP-1 on Leydig cell regeneration from stem cells in vivo and on Leydig cell 
      development in vitro. RESULTS: Intratesticular injection of MCP-1(10 ng/testis) 
      into Leydig cell-depleted rat testis from post-EDS day 14 to 28 significantly 
      increased serum testosterone and luteinizing hormone levels, up-regulated the 
      expression of Leydig cell proteins, LHCGR, SCARB1, CYP11A1, HSD3B1, CYP17A1, and 
      HSD17B3 without affecting progenitor Leydig cell proliferation, as well as 
      increased ERK1/2 phosphorylation. MCP-1 (100 ng/ml) significantly increased 
      medium testosterone levels and up-regulated LHCGR, CYP11A1, and HSD3B1 expression 
      without affecting EdU incorporation into stem cells after in vitro culture for 
      7 days. RS102895, a CCR2 inhibitor, reversed MCP-1-mediated increase of 
      testosterone level after culture in combination with MCP-1. CONCLUSION: MCP-1 
      stimulates the differentiation of stem and progenitor Leydig cells without 
      affecting their proliferation.
FAU - Zhan, Xiangcheng
AU  - Zhan X
AD  - Department of Urology, Shanghai Tenth People's Hospital, Tongji University School 
      of Medicine, Shanghai, 200072, China.
AD  - Tongji University School of Medicine, Shanghai, 200092, China.
FAU - Zhang, Jingwei
AU  - Zhang J
AD  - Department of Urology, Yijishan Hospital, Wannan Medical College, Wuhu, 241000, 
      Anhui, China.
FAU - Li, Saiyang
AU  - Li S
AD  - Department of Urology, Shanghai Tenth People's Hospital, Tongji University School 
      of Medicine, Shanghai, 200072, China.
AD  - Nanjing Medical University, Nanjing, China.
FAU - Zhang, Xiaolu
AU  - Zhang X
AD  - Department of Urology, Shanghai Tenth People's Hospital, Tongji University School 
      of Medicine, Shanghai, 200072, China.
FAU - Li, Linchao
AU  - Li L
AD  - Department of Anesthesiology, the Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, 
      China.
FAU - Song, Tiantian
AU  - Song T
AD  - Department of Anesthesiology, the Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, 
      China.
FAU - Liu, Qunlong
AU  - Liu Q
AD  - Department of Urology, Shanghai Tenth People's Hospital, Tongji University School 
      of Medicine, Shanghai, 200072, China.
AD  - Nanjing Medical University, Nanjing, China.
FAU - Lu, Jun
AU  - Lu J
AD  - Department of Urology, Shanghai Tenth People's Hospital, Tongji University School 
      of Medicine, Shanghai, 200072, China.
AD  - Department of Anesthesiology, the Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, 
      China.
FAU - Xu, Yunfei
AU  - Xu Y
AD  - Department of Urology, Shanghai Tenth People's Hospital, Tongji University School 
      of Medicine, Shanghai, 200072, China. xuyunfeibb@sina.com.
AD  - Tongji University School of Medicine, Shanghai, 200092, China. 
      xuyunfeibb@sina.com.
AD  - Nanjing Medical University, Nanjing, China. xuyunfeibb@sina.com.
FAU - Ge, Ren-Shan
AU  - Ge RS
AUID- ORCID: 0000-0002-3448-9823
AD  - Department of Anesthesiology, the Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, 
      China. r_ge@yahoo.com.
LA  - eng
GR  - 81671446/National Natural Science Foundation of China/International
GR  - 81730042/National Natural Science Foundation of China/International
GR  - ZS2017009/Wenzhou Municipal Science and Technology Bureau/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201006
PL  - England
TA  - BMC Dev Biol
JT  - BMC developmental biology
JID - 100966973
RN  - 0 (Chemokine CCL2)
RN  - 3XMK78S47O (Testosterone)
RN  - 9002-67-9 (Luteinizing Hormone)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/*drug effects
MH  - Chemokine CCL2/*pharmacology
MH  - Gene Expression/drug effects
MH  - Leydig Cells/*cytology/drug effects/metabolism
MH  - Luteinizing Hormone/blood
MH  - Male
MH  - Rats
MH  - Regeneration/*drug effects
MH  - Stem Cells/*cytology/drug effects/metabolism
MH  - Testis/drug effects/*physiology
MH  - Testosterone/blood
PMC - PMC7541273
OTO - NOTNLM
OT  - Differentiation
OT  - Monocyte chemoattractant protein-1
OT  - Proliferation
OT  - Stem Leydig cells
OT  - Testosterone
COIS- The authors declare no competing interests.
EDAT- 2020/10/08 06:00
MHDA- 2021/09/28 06:00
PMCR- 2020/10/06
CRDT- 2020/10/07 05:32
PHST- 2019/11/26 00:00 [received]
PHST- 2020/09/20 00:00 [accepted]
PHST- 2020/10/07 05:32 [entrez]
PHST- 2020/10/08 06:00 [pubmed]
PHST- 2021/09/28 06:00 [medline]
PHST- 2020/10/06 00:00 [pmc-release]
AID - 10.1186/s12861-020-00225-1 [pii]
AID - 225 [pii]
AID - 10.1186/s12861-020-00225-1 [doi]
PST - epublish
SO  - BMC Dev Biol. 2020 Oct 6;20(1):20. doi: 10.1186/s12861-020-00225-1.