PMID- 33025710
OWN - NLM
STAT- MEDLINE
DCOM- 20210428
LR  - 20210428
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 130
IP  - 4
DP  - 2021 Apr
TI  - Non-sterile corn steep liquor a novel, cost effective and powerful culture media 
      for Sporosarcina pasteurii cultivation for sand improvement.
PG  - 1232-1244
LID - 10.1111/jam.14866 [doi]
AB  - AIMS: Microbial induced calcium carbonate precipitation (MICP) is one of the 
      bio-cementation methods for improving granular soils. This study evaluate the 
      feasibility of obtaining a bacterial solution with high optical density and 
      urease activity by an inexpensive corn steep liquor (CSL) medium in non-sterile 
      conditions in order to achieve sand improvement. METHODS AND RESULTS: Corn steep 
      liquor media with different concentrations (different dilution rates) were 
      prepared and, without any autoclaving (non-sterile conditions), different 
      percentage of the inoculum solutions were added to them and incubated. Effect of 
      inoculum solution percentage and CSL dilution rates on specifications of 
      bacterial solution was evaluated. Urease activity and scanning electron 
      microscope (SEM) and X-Ray Diffraction (XRD) were used to efficiency of CLS media 
      in sand improvement. The considerable urease activity was measured as 
      5.7 mS cm(-1)  min(-1) using nonsterile CLS. By using CYNU (CSL-Yeast 
      extract-NH4Cl-Urea) bacterial solution, the urease activity of 5.5 mS cm(-1) 
       min(-1) for the OD(600) (optical density at 600 nm) of 1.88 and, consequently, 
      specific urease activity of 2.93 mS cm(-1)  min(-1)  OD(600) (-1) was obtained. 
      The highest unconfined compressive strength (811 kPa) was obtained for the CYNU. 
      XRD revealed new calcite peaks next to the quartz peaks. CONCLUSIONS: Production 
      of inexpensive bacterial solution using diluted CSL as the inexpensive, effective 
      and powerful culture media for Sporosarcina pasteurii cultivation in nonsterile 
      conditions, allows geotechnical and biotechnological engineers to use MICP 
      technology more widely in land improvement and field-scale bio-cementation and 
      bioremediation projects. SIGNIFICANCE AND IMPACT OF THE STUDY: Obtaining high 
      urease activity of inexpensive microbial solution using diluted CSL as the 
      culture medium in nonsterile conditions, as the unique results of this study, can 
      be significant in the field of bioremediation studies using MICP.
CI  - (c) 2020 The Society for Applied Microbiology.
FAU - Babakhani, S
AU  - Babakhani S
AD  - University of Bonab, Bonab, Iran.
FAU - Fahmi, A
AU  - Fahmi A
AD  - Department of Civil Engineering, Faculty of Engineering, University of Bonab, 
      Bonab, Iran.
FAU - Katebi, H
AU  - Katebi H
AD  - Faculty of Civil Engineering, University of Tabriz, Tabriz, Iran.
FAU - Ouria, A
AU  - Ouria A
AD  - Department of Civil Engineering, University of Mohaghegh Ardabili, Ardabil, Iran.
FAU - Majnouni-Toutakhane, A
AU  - Majnouni-Toutakhane A
AD  - Department of Architecture, University of Bonab, Bonab, Iran.
FAU - Ganbarov, K
AU  - Ganbarov K
AD  - Department of Microbiology, Baku State University, Baku, Azerbaijan.
FAU - Kafil, H S
AU  - Kafil HS
AUID- ORCID: 0000-0001-6026-8795
AD  - Drug Applied Research Center, Faculty of Medicine, Tabriz University of Medical 
      Science, Tabriz, Iran.
LA  - eng
GR  - Tabriz University of Medical Sciences/
PT  - Journal Article
DEP - 20201007
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (Culture Media)
RN  - 0 (Sand)
RN  - EC 3.5.1.5 (Urease)
RN  - H0G9379FGK (Calcium Carbonate)
RN  - Sporosarcina pasteurii
SB  - IM
MH  - Biodegradation, Environmental
MH  - Biomineralization
MH  - Calcium Carbonate/analysis/metabolism
MH  - Compressive Strength
MH  - Cost-Benefit Analysis
MH  - Culture Media/chemistry
MH  - Sand/*chemistry/microbiology
MH  - Sporosarcina/*growth & development/metabolism
MH  - Urease/metabolism
MH  - Zea mays/*chemistry
OTO - NOTNLM
OT  - bio-cementation
OT  - bioremediation
OT  - calcium carbonate precipitation
OT  - corn steep liquor
OT  - sand improvement
EDAT- 2020/10/08 06:00
MHDA- 2021/04/29 06:00
CRDT- 2020/10/07 05:57
PHST- 2020/06/30 00:00 [received]
PHST- 2020/09/07 00:00 [revised]
PHST- 2020/09/17 00:00 [accepted]
PHST- 2020/10/08 06:00 [pubmed]
PHST- 2021/04/29 06:00 [medline]
PHST- 2020/10/07 05:57 [entrez]
AID - 10.1111/jam.14866 [doi]
PST - ppublish
SO  - J Appl Microbiol. 2021 Apr;130(4):1232-1244. doi: 10.1111/jam.14866. Epub 2020 
      Oct 7.