PMID- 33025741
OWN - NLM
STAT- MEDLINE
DCOM- 20210623
LR  - 20210623
IS  - 1864-0648 (Electronic)
IS  - 1864-063X (Linking)
VI  - 13
IP  - 12
DP  - 2020 Dec
TI  - Immunofluorescence and histopathological assessment using ex vivo confocal laser 
      scanning microscopy in lichen planus.
PG  - e202000328
LID - 10.1002/jbio.202000328 [doi]
AB  - Ex vivo confocal laser scanning microscopy (CLSM) provides rapid, high-resolution 
      imaging, fluorescence detection and digital haematoxylin-eosin (H&E)-like 
      staining. We aimed to assess the performance of ex vivo CLSM in identifying 
      histomorphology and immunoreactivity in lichen planus (LP) and comparing its 
      accuracy with conventional histopathology and direct immunofluorescence (DIF). 
      Thirty-three sections of 17 LP patients stained with acridine orange (AO) and 
      FITC-labelled anti-fibrinogen antibody and 21 control samples stained with AO 
      were examined using ex vivo CLSM. Ex vivo CLSM was in perfect agreement with 
      conventional histopathology in identifying interface dermatitis, vacuolar 
      degeneration and band-like infiltration. ROC analysis showed that the presence of 
      vacuolar degeneration, interface dermatitis and band-like infiltration was useful 
      to distinguish LP sections from controls (p < .0001). The detection rates of 
      fibrinogen deposition using DIF and in conclusion ex vivo CLSM were 93.8% and 
      62.5%, respectively. ex vivo CLSM enables histopathological and 
      immunofluorescence examination in LP with the advantage of digital H&E-like 
      staining.
CI  - (c) 2020 Wiley-VCH GmbH.
FAU - Bagci, Isin Sinem
AU  - Bagci IS
AUID- ORCID: 0000-0002-9254-8588
AD  - Department of Dermatology, Stanford University School of Medicine, Stanford, 
      California, USA.
FAU - Aoki, Rui
AU  - Aoki R
AD  - Department of Dermatology and Allergy, University Hospital, LMU, Munich, Germany.
FAU - Krammer, Sebastian
AU  - Krammer S
AUID- ORCID: 0000-0001-7968-9821
AD  - Department of Dermatology and Allergy, University Hospital, LMU, Munich, Germany.
FAU - Vladimirova, Gabriela
AU  - Vladimirova G
AD  - Department of Dermatology and Allergy, University Hospital, LMU, Munich, Germany.
FAU - Ruzicka, Thomas
AU  - Ruzicka T
AD  - Department of Dermatology and Allergy, University Hospital, LMU, Munich, Germany.
FAU - Sardy, Miklos
AU  - Sardy M
AD  - Department of Dermatology and Allergy, University Hospital, LMU, Munich, Germany.
AD  - Department of Dermatology, Venereology and Dermatooncology, Faculty of Medicine, 
      Semmelweis University, Budapest, Hungary.
FAU - French, Lars E
AU  - French LE
AD  - Department of Dermatology and Allergy, University Hospital, LMU, Munich, Germany.
FAU - Hartmann, Daniela
AU  - Hartmann D
AD  - Department of Dermatology and Allergy, University Hospital, LMU, Munich, Germany.
LA  - eng
PT  - Journal Article
DEP - 20201026
PL  - Germany
TA  - J Biophotonics
JT  - Journal of biophotonics
JID - 101318567
SB  - IM
MH  - Fluorescent Antibody Technique
MH  - Fluorescent Antibody Technique, Direct
MH  - Humans
MH  - *Lichen Planus/diagnostic imaging
MH  - Microscopy, Confocal
MH  - Staining and Labeling
OTO - NOTNLM
OT  - diagnostics
OT  - direct immunofluorescence microscopy
OT  - fluorescence
OT  - lichen planus
EDAT- 2020/10/08 06:00
MHDA- 2021/06/24 06:00
CRDT- 2020/10/07 05:57
PHST- 2020/08/14 00:00 [received]
PHST- 2020/09/10 00:00 [revised]
PHST- 2020/09/24 00:00 [accepted]
PHST- 2020/10/08 06:00 [pubmed]
PHST- 2021/06/24 06:00 [medline]
PHST- 2020/10/07 05:57 [entrez]
AID - 10.1002/jbio.202000328 [doi]
PST - ppublish
SO  - J Biophotonics. 2020 Dec;13(12):e202000328. doi: 10.1002/jbio.202000328. Epub 
      2020 Oct 26.