PMID- 33025745 OWN - NLM STAT- MEDLINE DCOM- 20211014 LR - 20211014 IS - 1932-7005 (Electronic) IS - 1932-6254 (Linking) VI - 14 IP - 12 DP - 2020 Dec TI - Nanographene oxide-calcium phosphate to inhibit Staphylococcus aureus infection and support stem cells for bone tissue engineering. PG - 1779-1791 LID - 10.1002/term.3139 [doi] AB - Staphylococcus aureus (S. aureus) is a major pathogen for osteomyelitis. Calcium phosphate bone cement (CPC) paste is promising for orthopedic uses. Nanostructured graphene oxide (GO) showed antibacterial effect on Gram-positive bacteria. However, there has been no report of incorporating GO into CPC. The objectives of this study were to (a) develop an injectable and mechanically strong CPC-chitosan paste containing GO and (b) investigate the inhibition of S. aureus infection and the promotion of human umbilical cord mesenchymal stem cells (hUCMSCs) for bone regeneration. Injectable CPC-chitosan-GO paste was fabricated. Flexural strength, elastic modulus, and work-of-fracture of the CPC-chitosan and CPC-chitosan-GO bars were evaluated. Antibacterial effects against S. aureus biofilms were determined. hUCMSC growth and viability on disks were investigated. CPC-chitosan-GO bars had a flexural strength of 7.2 +/- 1.6 MPa, matching that of CPC-chitosan control without GO. CPC-chitosan-GO had strong antibacterial effects on S. aureus, with an inhibition zone of 55.2 +/- 2.5 mm, greater than that of CPC-chitosan control (30.1 +/- 2.0 mm) (p < 0.05). CPC-chitosan-GO had potent antibacterial activity on S. aureus biofilms in vitro (p > 0.05). The injectable and antibacterial CPC-GO paste had no toxic effect, yielding excellent hUCMSC growth and viability on disks. The CPC-chitosan-GO had injectability, good strength, strong antibacterial effects, and excellent stem cell attachment and growth. CPC-chitosan-GO is promising for dental, craniofacial, and orthopedic applications to control infections and good biocompatibility to support stem cell viability to enhance bone regeneration. CI - (c) 2020 John Wiley & Sons, Ltd. FAU - Wu, Shizhou AU - Wu S AD - Department of Orthopedic Surgery, West China Hospital, Sichuan University, Chengdu, China. AD - Biomaterials and Tissue Engineering Division, Department of Advanced Oral Sciences and Therapeutics, University of Maryland Dental School, Baltimore, MD, USA. FAU - Lei, Lei AU - Lei L AD - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China. FAU - Zhang, Hui AU - Zhang H AD - Department of Orthopedic Surgery, West China Hospital, Sichuan University, Chengdu, China. FAU - Liu, Jin AU - Liu J AD - Biomaterials and Tissue Engineering Division, Department of Advanced Oral Sciences and Therapeutics, University of Maryland Dental School, Baltimore, MD, USA. AD - Key Laboratory of Shannxi Province for Craniofacial Precision Medicine Research, Clinical Research Center of Shannxi Province for Dental and Maxillofacial Diseases, College of Stomatology, Xi'an Jiaotong University, Xi'an, China. FAU - Weir, Michael D AU - Weir MD AD - Biomaterials and Tissue Engineering Division, Department of Advanced Oral Sciences and Therapeutics, University of Maryland Dental School, Baltimore, MD, USA. FAU - Schneider, Abraham AU - Schneider A AD - Department of Oncology and Diagnostic Sciences, University of Maryland School of Dentistry, Baltimore, MD, USA. FAU - Zhao, Liang AU - Zhao L AD - Department of Orthopedic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China. FAU - Liu, Jun AU - Liu J AUID- ORCID: 0000-0001-5072-3205 AD - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China. FAU - Xu, Hockin H K AU - Xu HHK AUID- ORCID: 0000-0001-5160-8866 AD - Biomaterials and Tissue Engineering Division, Department of Advanced Oral Sciences and Therapeutics, University of Maryland Dental School, Baltimore, MD, USA. AD - Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, MD, USA. AD - Center for Stem Cell Biology and Regenerative Medicine, University of Maryland School of Medicine, Baltimore, MD, USA. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20201006 PL - England TA - J Tissue Eng Regen Med JT - Journal of tissue engineering and regenerative medicine JID - 101308490 RN - 0 (Anti-Bacterial Agents) RN - 0 (Bone Cements) RN - 0 (Calcium Phosphates) RN - 0 (graphene oxide) RN - 7782-42-5 (Graphite) RN - 9012-76-4 (Chitosan) RN - 97Z1WI3NDX (calcium phosphate) SB - IM MH - Anti-Bacterial Agents/pharmacology MH - Bone Cements/pharmacology MH - Bone and Bones/*physiology MH - Calcium Phosphates/*pharmacology MH - Cell Survival/drug effects MH - Chitosan/pharmacology MH - Graphite/*pharmacology MH - Humans MH - Microbial Sensitivity Tests MH - Nanoparticles/*chemistry MH - Staphylococcal Infections/*microbiology MH - Staphylococcus aureus/*drug effects MH - Stem Cells/*cytology/drug effects MH - Surface Properties MH - *Tissue Engineering MH - Umbilical Cord/cytology OTO - NOTNLM OT - Staphylococcus aureus OT - antibacterial OT - calcium phosphate OT - graphene oxide OT - injectable OT - stem cells EDAT- 2020/10/08 06:00 MHDA- 2021/10/15 06:00 CRDT- 2020/10/07 05:57 PHST- 2020/03/02 00:00 [received] PHST- 2020/09/03 00:00 [revised] PHST- 2020/09/08 00:00 [accepted] PHST- 2020/10/08 06:00 [pubmed] PHST- 2021/10/15 06:00 [medline] PHST- 2020/10/07 05:57 [entrez] AID - 10.1002/term.3139 [doi] PST - ppublish SO - J Tissue Eng Regen Med. 2020 Dec;14(12):1779-1791. doi: 10.1002/term.3139. Epub 2020 Oct 6.