PMID- 33026392
OWN - NLM
STAT- MEDLINE
DCOM- 20210330
LR  - 20210330
IS  - 2050-7518 (Electronic)
IS  - 2050-750X (Linking)
VI  - 8
IP  - 38
DP  - 2020 Oct 14
TI  - Temperature-triggered attachment and detachment of general human bio-foulants on 
      zwitterionic polydimethylsiloxane.
PG  - 8853-8863
LID - 10.1039/d0tb01478h [doi]
AB  - Biofouling has long been a problem for biomaterials, so being able to control the 
      fouling on the surface of a biomaterial would be ideal. In this study a copolymer 
      system was designed comprising three moieties: an epoxy containing group, 
      glycidyl methacrylate (GMA); a thermoresponsive segment, N-isopropylacrylamide 
      (NIPAAm); and an antifouling zwitterionic unit, sulfobetaine methacrylate (SBMA). 
      The copolymers (pGSN), synthesized via free radical polymerization with these 3 
      moieties, were then grafted onto polydimethylsiloxane (PDMS). The presence of a 
      critical temperature for both the copolymers and the coated PDMS was evidenced by 
      particle size and contact angle measurements. The coated PDMS exhibited 
      controllable temperature-dependent antifouling behaviors and stimuli-responsive 
      phase characteristics in the presence of salts. The interactions of the coated 
      PDMS with biomolecules were tested via attachment of fibrinogen protein, 
      platelets, human whole blood, and tumor cells (HT1080). The attachment and 
      detachment of these biomolecules were studied at different temperatures. Exposed 
      hydrophobic domains of thermoresponsive NIPAAm-rich pGSN containing NIPAAm at 56 
      mol% generally allows molecular and cellular attachment on the PDMS surface at 37 
       degrees C. On the other hand, the coated PDMS with a relatively high content of SBMA 
      (>41 mol%) in the copolymer started to exhibit fouling resistance and lower the 
      thermoresponsive properties. Interestingly, the incorporation of zwitterionic 
      SBMA units into the copolymers was found to accelerate the hydration of the PDMS 
      surfaces and resulted in biomolecular and cellular detachment at 25  degrees C, which is 
      comparable to the detachment at 4  degrees C. This modified surface behavior is found to 
      be consistent through all biofouling tests.
FAU - Tan, Christian Martin E
AU  - Tan CME
AD  - School of Chemical, Biological and Materials Engineering and Sciences, Mapua 
      University, Intramuros, Manila, 1002, Philippines.
FAU - Dizon, Gian Vincent
AU  - Dizon GV
FAU - Chen, Sheng-Han
AU  - Chen SH
FAU - Venault, Antoine
AU  - Venault A
FAU - Chou, Ying-Nien
AU  - Chou YN
FAU - Tayo, Lemmuel
AU  - Tayo L
FAU - Chang, Yung
AU  - Chang Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200902
PL  - England
TA  - J Mater Chem B
JT  - Journal of materials chemistry. B
JID - 101598493
RN  - 0 (Acrylamides)
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Epoxy Compounds)
RN  - 0 (Methacrylates)
RN  - 0 (Polymethacrylic Acids)
RN  - 9001-32-5 (Fibrinogen)
RN  - B7GFF17L9U (N-isopropylacrylamide)
RN  - R8WN29J8VF (glycidyl methacrylate)
SB  - IM
MH  - Acrylamides/chemistry
MH  - Adsorption
MH  - Biofouling/*prevention & control
MH  - Blood Platelets/metabolism
MH  - Cell Adhesion/drug effects
MH  - Cell Line
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Epoxy Compounds/chemistry
MH  - Fibrinogen/*chemistry
MH  - Humans
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Methacrylates/chemistry
MH  - Osmolar Concentration
MH  - Polymethacrylic Acids/*chemistry
MH  - Temperature
EDAT- 2020/10/08 06:00
MHDA- 2021/03/31 06:00
CRDT- 2020/10/07 12:09
PHST- 2020/10/08 06:00 [pubmed]
PHST- 2021/03/31 06:00 [medline]
PHST- 2020/10/07 12:09 [entrez]
AID - 10.1039/d0tb01478h [doi]
PST - ppublish
SO  - J Mater Chem B. 2020 Oct 14;8(38):8853-8863. doi: 10.1039/d0tb01478h. Epub 2020 
      Sep 2.