PMID- 33027874
OWN - NLM
STAT- MEDLINE
DCOM- 20201009
LR  - 20201009
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 746
DP  - 2020 Dec 1
TI  - Temporal transcriptome analysis in female scallop Chlamys farreri: First 
      molecular insights into the disturbing mechanism on lipid metabolism of 
      reproductive-stage dependence under benzo[a]pyrene exposure.
PG  - 142032
LID - S0048-9697(20)35561-3 [pii]
LID - 10.1016/j.scitotenv.2020.142032 [doi]
AB  - Polycyclic aromatic hydrocarbons (PAHs) are one of the most widespread persistent 
      organic pollutants (POPs) in marine environment. Benzo[a]pyrene (B[a]P), the most 
      toxic carcinogen of PAHs, is widely studied as a representative that interferes 
      with lipid metabolism. However, the underlying molecular mechanisms of lipid 
      metabolism by B[a]P interference towards bivalve, one of the marine-pollution 
      bio-indicators have not been elucidated yet, especially during gonadal 
      development which is closely associated with lipids. In this study, female 
      scallops Chlamys farreri were cultured with natural and 4 mug/L B[a]P exposed 
      seawater, respectively, and a multi-stage (proliferative, growth, mature, and 
      spawn stage) ovarian transcriptome profiling was performed to decipher the 
      reproductive stage-dependence disturbing mechanisms on lipid metabolism caused by 
      B[a]P in bivalves. The results revealed the potential molecular mechanism of 
      B[a]P-induced triglycerides (TGs) accumulation, which probably resulted from the 
      collaboration of promoting synthesis and inhibiting metabolization of TGs, 
      notably, this mechanism also occurred at spawn stage. Correspondingly, B[a]P and 
      TGs contents measured in ovary offered direct biochemical evidences for the 
      interference effects and stage-dependent accumulation patterns of B[a]P. 
      Moreover, the gene expressions of fatty acids synthesis related enzymes were 
      down-regulated cooperatively, illustrating the molecular compensatory mechanism 
      that reduced susceptibility from oxidative damage. And these results further 
      emphasized the important role of prostaglandins (PGs) in immune response mediated 
      by arachidonic acid metabolism. In addition, this study explored the underlying 
      molecular mechanism affected by B[a]P on sterol metabolism, which possibly posed 
      a threat to normal reproductive functions in bivalves. Taken together, our 
      findings filled the gap of the stage-dependent interference molecular mechanisms 
      on lipid metabolism behind bivalves, and provided a new perspective for 
      investigating the adaptive mechanisms of bivalves under POPs stress.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Xu, Ruiyi
AU  - Xu R
AD  - The Key Laboratory of Mariculture, Ministry of Education, Ocean University of 
      China, Qingdao 266003, PR China.
FAU - Pan, Luqing
AU  - Pan L
AD  - The Key Laboratory of Mariculture, Ministry of Education, Ocean University of 
      China, Qingdao 266003, PR China. Electronic address: panlq@ouc.edu.cn.
FAU - Yang, Yingying
AU  - Yang Y
AD  - The Key Laboratory of Mariculture, Ministry of Education, Ocean University of 
      China, Qingdao 266003, PR China.
FAU - Zhou, Yueyao
AU  - Zhou Y
AD  - The Key Laboratory of Mariculture, Ministry of Education, Ocean University of 
      China, Qingdao 266003, PR China.
FAU - Li, Dongyu
AU  - Li D
AD  - The Key Laboratory of Mariculture, Ministry of Education, Ocean University of 
      China, Qingdao 266003, PR China.
LA  - eng
PT  - Journal Article
DEP - 20200831
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 3417WMA06D (Benzo(a)pyrene)
SB  - IM
MH  - Animals
MH  - *Benzo(a)pyrene/toxicity
MH  - Female
MH  - Gene Expression Profiling
MH  - Lipid Metabolism
MH  - *Pectinidae/genetics
MH  - Reproduction
OTO - NOTNLM
OT  - Benzo[a]pyrene
OT  - Chlamys farreri
OT  - Fatty acids
OT  - Stage-dependent
OT  - Transcriptome
OT  - Triglycerides
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/10/09 06:00
MHDA- 2020/10/10 06:00
CRDT- 2020/10/08 01:01
PHST- 2020/07/18 00:00 [received]
PHST- 2020/08/26 00:00 [revised]
PHST- 2020/08/26 00:00 [accepted]
PHST- 2020/10/08 01:01 [entrez]
PHST- 2020/10/09 06:00 [pubmed]
PHST- 2020/10/10 06:00 [medline]
AID - S0048-9697(20)35561-3 [pii]
AID - 10.1016/j.scitotenv.2020.142032 [doi]
PST - ppublish
SO  - Sci Total Environ. 2020 Dec 1;746:142032. doi: 10.1016/j.scitotenv.2020.142032. 
      Epub 2020 Aug 31.