PMID- 33028328
OWN - NLM
STAT- MEDLINE
DCOM- 20210623
LR  - 20210623
IS  - 2662-7671 (Electronic)
IS  - 2662-7671 (Linking)
VI  - 20
IP  - 1
DP  - 2020 Oct 7
TI  - Isatis root polysaccharide promotes maturation and secretory function of 
      monocyte-derived dendritic cells.
PG  - 301
LID - 10.1186/s12906-020-03103-2 [doi]
LID - 301
AB  - BACKGROUND: Pseudorabies virus (PRV) is an animal virus that is globally 
      responsible for the high economic losses in the swine industry. Isatis root is a 
      traditional Chinese medicinal herb that possesses immune-enhancing and antiviral 
      properties. However, the molecular mechanisms underlying the effects of the 
      active component of the isatis root polysaccharide (IRPS) extract on immature 
      dendritic cells remain elusive. METHODS: In this study, we investigated the 
      molecular changes in primary porcine peripheral blood monocyte-derived dendritic 
      cells (MoDCs) during PRV infection, using enzyme-linked immunosorbent assay 
      (ELISA) and quantitative reverse transcription-polymerase chain reaction. 
      Additionally, we studied the effect of IRPS on PRV-infected DCs. RESULTS: The 
      results showed that IRPS stimulated the maturation of MoDCs, induced IL-12 
      secretion, and downregulated IL-6 expression. CONCLUSIONS: Collectively, these 
      results suggest that IRPS is a promising candidate for promoting maturation of 
      DCs and enhancing their secretory potential after PRV infection.
FAU - Wang, Xuebing
AU  - Wang X
AD  - College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450000, 
      Henan province, People's Republic of China.
AD  - Key Laboratory for Animal-Derived Food Safety of Henan province, Zhengzhou, 
      450000, Henan province, People's Republic of China.
FAU - Chen, Zewen
AU  - Chen Z
AD  - College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450000, 
      Henan province, People's Republic of China.
FAU - Chen, Tong
AU  - Chen T
AD  - College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450000, 
      Henan province, People's Republic of China.
FAU - Li, Xiao
AU  - Li X
AD  - Biotechnology Developing Center of Henan Academy of Sciences, Henan Academy of 
      sciences, Zhengzhou, 450002, Henan province, People's Republic of China.
FAU - Huang, Shucheng
AU  - Huang S
AD  - College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450000, 
      Henan province, People's Republic of China.
FAU - Wang, Hao
AU  - Wang H
AD  - College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450000, 
      Henan province, People's Republic of China.
FAU - Tong, Chao
AU  - Tong C
AUID- ORCID: 0000-0002-4528-5148
AD  - College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450000, 
      Henan province, People's Republic of China. chaotong@henau.edu.cn.
AD  - Wuhu Overseas Student Pioneer Park, Wuhu, 241006, China. chaotong@henau.edu.cn.
FAU - Liu, Fang
AU  - Liu F
AD  - College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450000, 
      Henan province, People's Republic of China. liufang.vet@henau.edu.cn.
LA  - eng
GR  - 172107000011/Henan Province Industry-University-Research Cooperation Project/
PT  - Journal Article
DEP - 20201007
PL  - England
TA  - BMC Complement Med Ther
JT  - BMC complementary medicine and therapies
JID - 101761232
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Plant Extracts)
RN  - 0 (Polysaccharides)
SB  - IM
MH  - Animals
MH  - China
MH  - Dendritic Cells/*drug effects
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Herpesvirus 1, Suid/*drug effects
MH  - *Isatis
MH  - Monocytes/*drug effects
MH  - Plant Extracts/*pharmacology
MH  - Plant Roots
MH  - Polysaccharides/*pharmacology
MH  - Swine
PMC - PMC7542110
OTO - NOTNLM
OT  - Isatis root polysaccharide
OT  - Monocyte-derived DCs
OT  - Pseudorabies virus
COIS- The authors declare that they have no competing interests.
EDAT- 2020/10/09 06:00
MHDA- 2021/06/24 06:00
PMCR- 2020/10/07
CRDT- 2020/10/08 05:24
PHST- 2020/03/14 00:00 [received]
PHST- 2020/10/01 00:00 [accepted]
PHST- 2020/10/08 05:24 [entrez]
PHST- 2020/10/09 06:00 [pubmed]
PHST- 2021/06/24 06:00 [medline]
PHST- 2020/10/07 00:00 [pmc-release]
AID - 10.1186/s12906-020-03103-2 [pii]
AID - 3103 [pii]
AID - 10.1186/s12906-020-03103-2 [doi]
PST - epublish
SO  - BMC Complement Med Ther. 2020 Oct 7;20(1):301. doi: 10.1186/s12906-020-03103-2.