PMID- 33030669
OWN - NLM
STAT- MEDLINE
DCOM- 20210729
LR  - 20210729
IS  - 1874-9356 (Electronic)
IS  - 0015-5632 (Print)
IS  - 0015-5632 (Linking)
VI  - 66
IP  - 1
DP  - 2021 Feb
TI  - Denaturing gradient gel electrophoresis and multi-SIR profiles of soil microbial 
      communities from a karst doline at Aggtelek National Park, Hungary.
PG  - 107-114
LID - 10.1007/s12223-020-00828-y [doi]
AB  - Soils play an important role in the ecosystem of karstic landscapes both as a 
      buffer zone and as a source of acidity to belowground water. Although the 
      microbiota of karstic soils is known to have a great effect on karstification 
      processes, the activity and composition of these communities are largely unknown. 
      This study gives a comparative analysis of soil microbial profiles from different 
      parts of a doline located at Aggtelek, Hungary. The aim was to reveal the 
      relationships between the vegetation type and genetic fingerprints and substrate 
      utilisation (multi-SIR) profiles of the soil microbiota. Soil samples were 
      collected in early and late springs along a transect in a doline covered with 
      different types of vegetation. Genetic fingerprints of bacterial communities were 
      examined by denaturing gradient gel electrophoresis (DGGE) based on the 16S rRNA 
      gene, along with multi-SIR profiles of the microbial communities measured by the 
      MicroResp method using 15 different carbon sources. Genetic fingerprinting 
      indicated that vegetation cover had a strong effect on the composition of soil 
      bacterial communities. Procrustean analysis showed only a weak connection between 
      DGGE and multi-SIR profiles, probably due to the high functional redundancy of 
      the communities. Seasonality had a significant effect on substrate usage, which 
      can be an important factor to consider in future studies.
FAU - Mucsi, Marton
AU  - Mucsi M
AUID- ORCID: 0000-0001-6226-0607
AD  - Institute for Soil Sciences and Agricultural Chemistry, Centre for Agricultural 
      Research, Herman Otto ut 15, Budapest, H-1022, Hungary.
AD  - Department of Microbiology, ELTE Eotvos Lorand University, Pazmany Peter setany 
      1/c, Budapest, H-1117, Hungary.
FAU - Krett, Gergely
AU  - Krett G
AD  - Department of Microbiology, ELTE Eotvos Lorand University, Pazmany Peter setany 
      1/c, Budapest, H-1117, Hungary.
FAU - Szili-Kovacs, Tibor
AU  - Szili-Kovacs T
AUID- ORCID: 0000-0002-8354-0656
AD  - Institute for Soil Sciences and Agricultural Chemistry, Centre for Agricultural 
      Research, Herman Otto ut 15, Budapest, H-1022, Hungary. 
      szili-kovacs.tibor@atk.hu.
FAU - Moga, Janos
AU  - Moga J
AUID- ORCID: 0000-0002-4471-4385
AD  - Department of Physical Geography, ELTE Eotvos Lorand University, Pazmany Peter 
      setany 1/c, Budapest, H-1117, Hungary.
FAU - Borsodi, Andrea K
AU  - Borsodi AK
AUID- ORCID: 0000-0002-3738-7937
AD  - Department of Microbiology, ELTE Eotvos Lorand University, Pazmany Peter setany 
      1/c, Budapest, H-1117, Hungary.
AD  - Danube Research Institute, Centre for Ecological Research, Karolina ut 29, 
      Budapest, H-1113, Hungary.
LA  - eng
GR  - K79135/OTKA/
PT  - Journal Article
DEP - 20201008
PL  - United States
TA  - Folia Microbiol (Praha)
JT  - Folia microbiologica
JID - 0376757
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Soil)
RN  - 7440-44-0 (Carbon)
SB  - IM
MH  - Bacteria/classification/genetics/metabolism
MH  - Carbon/metabolism
MH  - Cluster Analysis
MH  - Denaturing Gradient Gel Electrophoresis
MH  - Geological Phenomena
MH  - Hungary
MH  - Microbiota/*physiology
MH  - *Parks, Recreational
MH  - RNA, Ribosomal, 16S/genetics
MH  - Seasons
MH  - Soil/chemistry
MH  - *Soil Microbiology
PMC - PMC7854432
OTO - NOTNLM
OT  - Bacteria
OT  - DGGE
OT  - Karst soil
OT  - MicroResp
OT  - Multi-SIR
COIS- The authors declare no conflict of interest.
EDAT- 2020/10/09 06:00
MHDA- 2021/07/30 06:00
PMCR- 2020/10/08
CRDT- 2020/10/08 12:23
PHST- 2020/06/01 00:00 [received]
PHST- 2020/10/01 00:00 [accepted]
PHST- 2020/10/09 06:00 [pubmed]
PHST- 2021/07/30 06:00 [medline]
PHST- 2020/10/08 12:23 [entrez]
PHST- 2020/10/08 00:00 [pmc-release]
AID - 10.1007/s12223-020-00828-y [pii]
AID - 828 [pii]
AID - 10.1007/s12223-020-00828-y [doi]
PST - ppublish
SO  - Folia Microbiol (Praha). 2021 Feb;66(1):107-114. doi: 10.1007/s12223-020-00828-y. 
      Epub 2020 Oct 8.