PMID- 33034362
OWN - NLM
STAT- MEDLINE
DCOM- 20230929
LR  - 20230929
IS  - 1938-2391 (Electronic)
IS  - 1081-597X (Linking)
VI  - 36
IP  - 10
DP  - 2020 Oct 1
TI  - Evaluation of the Safety and Long-term Scleral Biomechanical Stability of UVA 
      Cross-linking on Scleral Collagen in Rhesus Monkeys.
PG  - 696-702
LID - 10.3928/1081597X-20200807-01 [doi]
AB  - PURPOSE: To investigate the changes of retinal and choroidal parameters, scleral 
      biomechanical strength, and ocular histopathology after scleral ultraviolet-A 
      (UVA) cross-linking (CXL) in rhesus monkeys eyes, and to evaluate the safety and 
      long-term biomechanical stability of scleral CXL for preventing myopia from 
      progressing further in clinic. METHODS: Six 3-year-old male rhesus monkeys (12 
      eyes) were randomized to receive UVA-CXL procedures applied on the superotemporal 
      equatorial sclera. Optical coherence tomography (OCT) and optical coherence 
      tomography angiography (OCTA) were used for examination before and 1 week and 1, 
      3, 6, and 12 months after CXL. The stress-strain behaviors of equatorial scleral 
      strips were analyzed 12 months postoperatively by a biomaterial tester. 
      Hematoxylin-eosin and terminal deoxynucleotidyl transferase dUTP nick-end 
      labeling (TUNEL) staining were performed 12 months postoperatively. RESULTS: For 
      central retinal thickness, choroidal thickness, and flow density of central 
      retinal superficial vascular networks, no statistical difference was noted 
      between CXL eyes and control eyes at 12 months postoperatively (P > .05). The 
      biomechanical stability of sclera was increased. The scleral stress and Young 
      modulus at 8% strain corresponded to 184% and 183%, respectively, of the control 
      values at 12 months (each P < .001). No retinal damage was detected on histology 
      in scleral CXL eyes. There was no obvious difference between scleral CXL eyes and 
      control eyes by hematoxylin-eosin and TUNEL staining (P > .05). CONCLUSIONS: 
      Scleral CXL with riboflavin/UVA in rhesus monkey eyes could strengthen the 
      biomechanical properties of scleral tissues and maintain the stability for 12 
      months postoperatively. The UVA-CXL on the sclera of rhesus monkey eyes seems to 
      be effective and safe. [J Refract Surg. 2020;36(10):696-702.].
CI  - Copyright 2020, SLACK Incorporated.
FAU - Sun, Mingshen
AU  - Sun M
FAU - Zhang, Fengju
AU  - Zhang F
FAU - Li, Yu
AU  - Li Y
FAU - Ouyang, Bowen
AU  - Ouyang B
FAU - Wang, Mengmeng
AU  - Wang M
FAU - Jiao, Xuan
AU  - Jiao X
FAU - Zhang, Li
AU  - Zhang L
FAU - Wang, Ningli
AU  - Wang N
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Refract Surg
JT  - Journal of refractive surgery (Thorofare, N.J. : 1995)
JID - 9505927
RN  - 9007-34-5 (Collagen)
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Photosensitizing Agents)
RN  - TLM2976OFR (Riboflavin)
SB  - IM
MH  - Animals
MH  - Male
MH  - Biomechanical Phenomena
MH  - Collagen
MH  - Cross-Linking Reagents
MH  - Macaca mulatta
MH  - *Photosensitizing Agents/pharmacology
MH  - Riboflavin/pharmacology
MH  - *Sclera
MH  - Ultraviolet Rays
EDAT- 2020/10/10 06:00
MHDA- 2021/08/19 06:00
CRDT- 2020/10/09 08:37
PHST- 2020/02/12 00:00 [received]
PHST- 2020/08/05 00:00 [accepted]
PHST- 2020/10/09 08:37 [entrez]
PHST- 2020/10/10 06:00 [pubmed]
PHST- 2021/08/19 06:00 [medline]
AID - 10.3928/1081597X-20200807-01 [doi]
PST - ppublish
SO  - J Refract Surg. 2020 Oct 1;36(10):696-702. doi: 10.3928/1081597X-20200807-01.