PMID- 33036194
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201103
IS  - 2076-2607 (Print)
IS  - 2076-2607 (Electronic)
IS  - 2076-2607 (Linking)
VI  - 8
IP  - 10
DP  - 2020 Oct 6
TI  - Development of an Enzyme-Linked Immunosorbent Assay to Determine the Expression 
      Dynamics of Ebola Virus Soluble Glycoprotein during Infection.
LID - 10.3390/microorganisms8101535 [doi]
LID - 1535
AB  - Ebola virus (EBOV) is a highly pathogenic virus with human case fatality rates of 
      up to 90%. EBOV uses transcriptional editing to express three different 
      glycoproteins (GPs) from its GP gene: soluble GP (sGP), GP, and small sGP (ssGP). 
      The molecular ratio of unedited to edited mRNA is about 70% (sGP): 25% (GP): 5% 
      (ssGP), indicating that sGP is produced more abundantly than GP. While the 
      presence of sGP has been confirmed in the blood during human EBOV infection, 
      there is no report about its expression dynamics. In this study, we developed an 
      EBOV-sGP-specific sandwich enzyme-linked immunosorbent assay (ELISA) using two 
      different available antibodies and tested several animal serum samples to 
      determine the concentration of sGP. EBOV-sGP was detected in nonhuman primate 
      serum samples as early as 4 days after EBOV infection, correlating with RT-qPCR 
      positivity. This ELISA might be further developed into a diagnostic tool for 
      detection of EBOV in patients. Furthermore, this study provides insights into the 
      expression dynamics of sGP during infection, which are important to decipher the 
      function that sGP plays during infection.
FAU - Furuyama, Wakako
AU  - Furuyama W
AD  - Laboratory of Virology, Division of Intramural Research, National Institute of 
      Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 
      59840, USA.
FAU - Marzi, Andrea
AU  - Marzi A
AUID- ORCID: 0000-0003-0186-9587
AD  - Laboratory of Virology, Division of Intramural Research, National Institute of 
      Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 
      59840, USA.
LA  - eng
GR  - IMVU/Division of Intramural Research, National Institute of Allergy and 
      Infectious Diseases/
PT  - Journal Article
DEP - 20201006
PL  - Switzerland
TA  - Microorganisms
JT  - Microorganisms
JID - 101625893
PMC - PMC7600751
OTO - NOTNLM
OT  - EBOV
OT  - ELISA
OT  - animal models
OT  - filovirus
OT  - sGP
COIS- The authors declare no conflict of interest.
EDAT- 2020/10/11 06:00
MHDA- 2020/10/11 06:01
PMCR- 2020/10/06
CRDT- 2020/10/10 01:01
PHST- 2020/09/17 00:00 [received]
PHST- 2020/10/02 00:00 [revised]
PHST- 2020/10/03 00:00 [accepted]
PHST- 2020/10/10 01:01 [entrez]
PHST- 2020/10/11 06:00 [pubmed]
PHST- 2020/10/11 06:01 [medline]
PHST- 2020/10/06 00:00 [pmc-release]
AID - microorganisms8101535 [pii]
AID - microorganisms-08-01535 [pii]
AID - 10.3390/microorganisms8101535 [doi]
PST - epublish
SO  - Microorganisms. 2020 Oct 6;8(10):1535. doi: 10.3390/microorganisms8101535.