PMID- 33038641
OWN - NLM
STAT- MEDLINE
DCOM- 20210617
LR  - 20210617
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 192
DP  - 2021 Jan 5
TI  - An SPRi-based biosensor pilot study: Analysis of multiple circulating 
      extracellular vesicles and hippocampal volume in Alzheimer's disease.
PG  - 113649
LID - S0731-7085(20)31535-1 [pii]
LID - 10.1016/j.jpba.2020.113649 [doi]
AB  - One of the main hurdles in the study of Alzheimer's Disease (AD) is the lack of 
      easily accessible and sensitive biomarkers for the diagnosis, the prediction of 
      the disease progression rate and the evaluation of rehabilitative and 
      pharmacological treatments. Extracellular Vesicles (EVs) are nanoscale particles 
      released by body cells, studied as promising biomarkers of AD as they are 
      involved in the onset and progression of the disease. In the strive for a 
      reliable and sensitive method to analyze EVs, we applied our recently developed 
      biosensor based on Surface Plasmon Resonance imaging (SPRi) technology for the 
      identification and profiling of neural EVs populations circulating in the plasma 
      of 10 AD patients and 10 healthy subjects. The SPRi-array was designed to 
      separate simultaneously EVs released by neurons, astrocytes, microglia and 
      oligodendrocytes, and to evaluate the presence and the relative amount of 
      specific surface molecules related to pathological processes including 
      translocator protein (TSPO), beta-Amyloid and ganglioside M1. As results, 
      significant variations in the relative amount and cargoes of specific 
      brain-derived populations of EVs were observed comparing EVs coming from AD 
      patients and healthy subjects, finding the main differences in the activation 
      phenotype of microglia EVs, in the lipid moieties on generic EVs and in the 
      beta-Amyloid expression on surfaces of neuronal EVs. Besides, the demonstrated 
      correlation of SPRi data with Magnetic Resonance Imaging analysis, provided 
      support for using the SPRi-based biosensor for the evaluation of 
      neurodegeneration detecting and characterizing circulating EVs as peripheral 
      biomarkers for the diagnosis and monitoring of progression and rehabilitation 
      treatments in AD patients.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Picciolini, Silvia
AU  - Picciolini S
AD  - IRCCS Fondazione Don Carlo Gnocchi, Via Capecelatro 66, Milan, Italy.
FAU - Gualerzi, Alice
AU  - Gualerzi A
AD  - IRCCS Fondazione Don Carlo Gnocchi, Via Capecelatro 66, Milan, Italy.
FAU - Carlomagno, Cristiano
AU  - Carlomagno C
AD  - IRCCS Fondazione Don Carlo Gnocchi, Via Capecelatro 66, Milan, Italy.
FAU - Cabinio, Monia
AU  - Cabinio M
AD  - IRCCS Fondazione Don Carlo Gnocchi, Via Capecelatro 66, Milan, Italy.
FAU - Sorrentino, Stefano
AU  - Sorrentino S
AD  - IRCCS Fondazione Don Carlo Gnocchi, Via Capecelatro 66, Milan, Italy.
FAU - Baglio, Francesca
AU  - Baglio F
AD  - IRCCS Fondazione Don Carlo Gnocchi, Via Capecelatro 66, Milan, Italy.
FAU - Bedoni, Marzia
AU  - Bedoni M
AD  - IRCCS Fondazione Don Carlo Gnocchi, Via Capecelatro 66, Milan, Italy. Electronic 
      address: mbedoni@dongnocchi.it.
LA  - eng
PT  - Journal Article
DEP - 20200923
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Receptors, GABA)
RN  - 0 (TSPO protein, human)
SB  - IM
MH  - *Alzheimer Disease/diagnostic imaging
MH  - *Extracellular Vesicles
MH  - Hippocampus/diagnostic imaging
MH  - Humans
MH  - Pilot Projects
MH  - Receptors, GABA
MH  - Surface Plasmon Resonance
OTO - NOTNLM
OT  - Alzheimer's disease
OT  - Biosensor
OT  - Extracellular vesicles
OT  - Hippocampal volume
OT  - Surface plasmon resonance imaging
COIS- Declaration of Competing Interest The authors reported no declarations of 
      interest.
EDAT- 2020/10/11 06:00
MHDA- 2021/06/22 06:00
CRDT- 2020/10/10 20:12
PHST- 2020/06/14 00:00 [received]
PHST- 2020/09/10 00:00 [revised]
PHST- 2020/09/15 00:00 [accepted]
PHST- 2020/10/11 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/10/10 20:12 [entrez]
AID - S0731-7085(20)31535-1 [pii]
AID - 10.1016/j.jpba.2020.113649 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2021 Jan 5;192:113649. doi: 10.1016/j.jpba.2020.113649. Epub 
      2020 Sep 23.