PMID- 33039524
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 451
DP  - 2020 Dec 15
TI  - A New Therapeutic Strategy Targeting Protein Deacetylation for Spinal Cord 
      Injury.
PG  - 197-206
LID - S0306-4522(20)30650-3 [pii]
LID - 10.1016/j.neuroscience.2020.09.060 [doi]
AB  - Lysine acetylation is a post-translational modification that regulates a 
      diversity of biological processes. However, its implication in spinal cord injury 
      (SCI) remains unclear. Here we investigated the acetylation events in injured 
      spinal cords on a proteomic scale for the first time. Additionally, whether 
      promoting acetylation could mitigate SCI was evaluated. A total of 268 
      differentially acetylated peptides were identified. Among them, 2 peptides were 
      up-acetylated and 141 peptides were down-acetylated in the injured spinal cord 
      tissues (Fold change >2 and P < 0.05). There were also 116 unique acetylated 
      peptides in the sham group and 9 unique acetylated peptides in the SCI group. 
      Functional enrichment analysis revealed that differently acetylated proteins were 
      involved in multiple cellular processes and metabolic processes. Kyoto 
      Encyclopaedia of Genes and Genomes analysis showed that several pathways, 
      including cGMP-PKG signaling pathway and hypoxia-inducible factor-1 (HIF-1) 
      signaling pathway, were predominantly presented. Moreover, promoting acetylation 
      using glycerol triacetate (GTA) showed a therapeutic effect on SCI, with improved 
      Basso-Beattie-Bresnahan scores and histologic morphology, and decreased neuronal 
      apoptosis and inflammation. In conclusion, our data indicated that protein 
      deacetylation might play crucial roles in the development of secondary injury of 
      SCI, and promoting acetylation by GTA effectively mitigated SCI. Our data not 
      only enhance our understanding on acetylproteome dataset in the spinal cord 
      tissues, but also provide novel insights for the treatment of SCI.
CI  - Copyright (c) 2020 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Liu, Xinwei
AU  - Liu X
AD  - Department of Orthopaedics, the General Hospital of Northern Theater Command, 
      Shenyang 110016, People's Republic of China.
FAU - Ma, Bin
AU  - Ma B
AD  - Division of Spine Surgery, Department of Orthopaedics, Tongji Hospital, Tongji 
      University School of Medicine, Shanghai 200065, People's Republic of China.
FAU - Zhang, Haocong
AU  - Zhang H
AD  - Department of Orthopaedics, the General Hospital of Northern Theater Command, 
      Shenyang 110016, People's Republic of China.
FAU - Chai, Ruonan
AU  - Chai R
AD  - Department of Respiratory Medicine, the General Hospital of Northern Theater 
      Command, Shenyang 110016, People's Republic of China. Electronic address: 
      lilypad_ff@126.com.
FAU - Xiang, Liangbi
AU  - Xiang L
AD  - Department of Orthopaedics, the General Hospital of Northern Theater Command, 
      Shenyang 110016, People's Republic of China. Electronic address: 
      deformitya@126.com.
LA  - eng
PT  - Journal Article
DEP - 20201009
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
SB  - IM
MH  - Acetylation
MH  - Humans
MH  - *Proteomics
MH  - Signal Transduction
MH  - Spinal Cord
MH  - *Spinal Cord Injuries/drug therapy
OTO - NOTNLM
OT  - acetylome
OT  - glycerol triacetate
OT  - lysine acetylation
OT  - spinal cord injury
EDAT- 2020/10/12 06:00
MHDA- 2021/05/15 06:00
CRDT- 2020/10/11 20:23
PHST- 2020/04/14 00:00 [received]
PHST- 2020/09/27 00:00 [revised]
PHST- 2020/09/29 00:00 [accepted]
PHST- 2020/10/12 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2020/10/11 20:23 [entrez]
AID - S0306-4522(20)30650-3 [pii]
AID - 10.1016/j.neuroscience.2020.09.060 [doi]
PST - ppublish
SO  - Neuroscience. 2020 Dec 15;451:197-206. doi: 10.1016/j.neuroscience.2020.09.060. 
      Epub 2020 Oct 9.