PMID- 33039542
OWN - NLM
STAT- Publisher
LR  - 20240222
IS  - 1872-8359 (Electronic)
IS  - 0167-7012 (Linking)
VI  - 178
DP  - 2020 Oct 8
TI  - Evaluation of a new chromogenic agar for the detection of environmental 
      Enterococcus.
PG  - 106082
LID - S0167-7012(20)30798-3 [pii]
LID - 10.1016/j.mimet.2020.106082 [doi]
AB  - CHROMagar Enterococcus (CHR), a new chromogenic medium not yet available for 
      commercial purchase, was evaluated for the isolation of Enterococcus from 
      environmental water samples. Its performance was evaluated in comparison to 
      commercially available media, Enterococcosel agar and m-Enterococcus agar. Three 
      consecutive tests were conducted with each test being performed with a newer 
      batch of the CHR medium with improved media composition per batch. The recovery 
      rate, positive predictive value, and sensitivity of the CHR medium improved with 
      the subsequent re-formulation of the media components from 93.9%, 63%, and 92.6%, 
      respectively, with the first batch of CHR, to 96.2%, 97.4%, and 95.7%, 
      respectively, with the newest batch of CHR. The results showed that the newer 
      batches of CHR performed better than the previous versions and are comparable to 
      the other two commercial media tested. The CHR medium has been developed to 
      decrease the turnaround time to approximately 18 h and be read more easily due to 
      bigger colony morphology. The superior growth of colonies on CHR compared with 
      other media in a shorter period of time can aid in the early detection of 
      enterococci and may offer a user-friendly alternative to other media for the 
      isolation of enterococci.
CI  - Published by Elsevier B.V.
FAU - Cho, Sohyun
AU  - Cho S
AD  - Bacterial Epidemiology and Antimicrobial Resistance Research Unit, United States 
      Department of Agriculture, Agricultural Research Service, Athens, GA 30605, USA. 
      Electronic address: Sohyun.Cho@usda.gov.
FAU - Hiott, Lari M
AU  - Hiott LM
AD  - Bacterial Epidemiology and Antimicrobial Resistance Research Unit, United States 
      Department of Agriculture, Agricultural Research Service, Athens, GA 30605, USA. 
      Electronic address: Lari.Hiott@usda.gov.
FAU - Woodley, Tiffanie A
AU  - Woodley TA
AD  - Bacterial Epidemiology and Antimicrobial Resistance Research Unit, United States 
      Department of Agriculture, Agricultural Research Service, Athens, GA 30605, USA. 
      Electronic address: Tiffanie.Woodley@usda.gov.
FAU - Frye, Jonathan G
AU  - Frye JG
AD  - Bacterial Epidemiology and Antimicrobial Resistance Research Unit, United States 
      Department of Agriculture, Agricultural Research Service, Athens, GA 30605, USA. 
      Electronic address: Jonathan.Frye@usda.gov.
FAU - Jackson, Charlene R
AU  - Jackson CR
AD  - Bacterial Epidemiology and Antimicrobial Resistance Research Unit, United States 
      Department of Agriculture, Agricultural Research Service, Athens, GA 30605, USA. 
      Electronic address: Charlene.Jackson@usda.gov.
LA  - eng
PT  - Journal Article
DEP - 20201008
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
SB  - IM
OTO - NOTNLM
OT  - Antimicrobial resistance
OT  - Diversity
OT  - Enterococcus
OT  - Environment
OT  - Media
OT  - Water
COIS- Declaration of Competing Interest The authors declare that there is no conflict 
      of interest. The mention of trade names or commercial products in this manuscript 
      is solely for the purpose of providing specific information and does not imply 
      recommendation or endorsement by the U.S. Department of Agriculture.
EDAT- 2020/10/12 06:00
MHDA- 2020/10/12 06:00
CRDT- 2020/10/11 20:24
PHST- 2020/08/26 00:00 [received]
PHST- 2020/10/02 00:00 [revised]
PHST- 2020/10/03 00:00 [accepted]
PHST- 2020/10/12 06:00 [pubmed]
PHST- 2020/10/12 06:00 [medline]
PHST- 2020/10/11 20:24 [entrez]
AID - S0167-7012(20)30798-3 [pii]
AID - 10.1016/j.mimet.2020.106082 [doi]
PST - aheadofprint
SO  - J Microbiol Methods. 2020 Oct 8;178:106082. doi: 10.1016/j.mimet.2020.106082.