PMID- 33039604
OWN - NLM
STAT- MEDLINE
DCOM- 20211104
LR  - 20211104
IS  - 1567-7257 (Electronic)
IS  - 1567-1348 (Linking)
VI  - 85
DP  - 2020 Nov
TI  - A simple method to generate PCR-RFLP typing profiles from DNA sequences in 
      Toxoplasma gondii.
PG  - 104590
LID - S1567-1348(20)30421-4 [pii]
LID - 10.1016/j.meegid.2020.104590 [doi]
AB  - Multilocus polymerase chain reaction-restriction fragment length polymorphism 
      (PCR-RFLP) has been widely used to genotype microorganisms. Accumulation of data 
      generated by this method has helped to reveal genetic diversity, population 
      structure and transmission of many microbial pathogens. Advances in DNA 
      sequencing technologies have made it possible to identify microorganisms by 
      multilocus sequencing typing (MLST) or whole genome sequence typing (WGST) to 
      reach high resolution of identification. While MLST and WGST are gradually 
      replacing PCR-RFLP for genotyping, invaluable databases generated by the latter 
      may not be easily linked to datasets generated by sequencing based methods. In 
      addition, DNA sequences corresponding to PCR-RFLP markers are often deposited in 
      public domains but not fully explored to infer the RFLP profile. To alleviate 
      this problem, we developed a simple protocol that can generate PCR-RFLP profiles 
      from DNA sequence data, therefore facilitating the integration of data generated 
      by different typing methods. Here we used the protozoan parasite Toxoplasma 
      gondii as an example to bridge different typing methods.
CI  - Copyright (c) 2020. Published by Elsevier B.V.
FAU - Castro, Bruno Bello Pede
AU  - Castro BBP
AD  - Department of Preventive Veterinary Medicine and Animal Health, Faculty of 
      Veterinary Medicine, University of Sao Paulo, Av. Prof. Orlando M. de Paiva, 87, 
      Cidade Universitaria, CEP 05408-270 Sao Paulo, SP, Brazil. Electronic address: 
      brunobellopede@usp.br.
FAU - Gennari, Solange Maria
AU  - Gennari SM
AD  - Department of Preventive Veterinary Medicine and Animal Health, Faculty of 
      Veterinary Medicine, University of Sao Paulo, Av. Prof. Orlando M. de Paiva, 87, 
      Cidade Universitaria, CEP 05408-270 Sao Paulo, SP, Brazil; Postgraduate Program 
      in Veterinary Medicine, Santo Amaro University, Av. Eneas de Siqueira Neto, 340, 
      CEP 04829-300, Sao Paulo, SP, Brazil. Electronic address: sgennari@usp.br.
FAU - Lorenzi, Hernan
AU  - Lorenzi H
AD  - The J. Craig Venter Institute, Rockville, MD 20895. USA. Electronic address: 
      hlorenzi@jcvi.org.
FAU - Su, Chunlei
AU  - Su C
AD  - Department of Microbiology, The University of Tennessee, Knoxville, TN 37996, 
      USA. Electronic address: csu1@utk.edu.
LA  - eng
GR  - U19 AI110819/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20201008
PL  - Netherlands
TA  - Infect Genet Evol
JT  - Infection, genetics and evolution : journal of molecular epidemiology and 
      evolutionary genetics in infectious diseases
JID - 101084138
RN  - 0 (DNA, Protozoan)
SB  - IM
MH  - *DNA, Protozoan
MH  - Genome, Protozoan
MH  - Genomics/methods
MH  - Genotype
MH  - Humans
MH  - Multilocus Sequence Typing
MH  - *Polymerase Chain Reaction/methods
MH  - *Polymorphism, Restriction Fragment Length
MH  - Toxoplasma/*classification/*genetics
MH  - Toxoplasmosis/*diagnosis/*parasitology
OTO - NOTNLM
OT  - Genotype
OT  - RFLP
OT  - Toxoplasma gondii
OT  - Whole genome sequence
EDAT- 2020/10/12 06:00
MHDA- 2021/11/05 06:00
CRDT- 2020/10/11 20:24
PHST- 2020/07/24 00:00 [received]
PHST- 2020/09/27 00:00 [revised]
PHST- 2020/10/05 00:00 [accepted]
PHST- 2020/10/12 06:00 [pubmed]
PHST- 2021/11/05 06:00 [medline]
PHST- 2020/10/11 20:24 [entrez]
AID - S1567-1348(20)30421-4 [pii]
AID - 10.1016/j.meegid.2020.104590 [doi]
PST - ppublish
SO  - Infect Genet Evol. 2020 Nov;85:104590. doi: 10.1016/j.meegid.2020.104590. Epub 
      2020 Oct 8.