PMID- 33042468
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201013
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 12
IP  - 9
DP  - 2020
TI  - CD47 antibody suppresses isoproterenol-induced cardiac hypertrophy through 
      activation of autophagy.
PG  - 5908-5923
AB  - In this study, we investigated whether CD47 antibody could attenuate 
      isoproterenol (ISO)-induced cardiac hypertrophy in mice and H9C2 cells. Cardiac 
      hypertrophy was induced by intraperitoneal (i.p.) injection of ISO (60 
      mg.kg(-1).d(-1) in 100 microl of sterile normal saline) daily for 14 days, and cell 
      hypertrophy was induced by ISO (10(-5) mol/l) for 48 h. The injury was confirmed 
      by increased levels of lactate dehydrogenase (LDH) and creatine kinase MB 
      (CK-MB), increased heart-to-body weight (HW/BW) ratio and visible cardiac 
      fibrosis. Apoptosis was evaluated by terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) staining. Autophagic flux in 
      H9c2 cells was monitored by TEM and mRFP-GFP-LC3 virus transfection. The 
      expression levels of Cleaved caspase-3, Cleaved caspase-9 and autophagy-related 
      proteins were detected by Western blotting. CD47 antibody significantly limited 
      ISO-induced increases in LDH, CK-MB, HW/BW ratio and attenuated cardiac fibrosis, 
      oxidative stress, and apoptosis in the heart; CD47 antibody promoted autophagy 
      flow and decreased cell apoptosis in cardiac tissues. Moreover, autophagy 
      inhibitor chloroquine (CQ) reversed the effect of CD47 antibody treatment. In 
      conclusion, CD47 antibody reduced ISO-induced cardiac hypertrophy by improving 
      autophagy flux and rescuing autophagic clearance.
CI  - AJTR Copyright (c) 2020.
FAU - Li, Yong
AU  - Li Y
AD  - Department of Cardiology, The First Affiliated Hospital of Nanjing Medical 
      University Nanjing 210029, Jiangsu Province, P. R. China.
FAU - Chen, Xuguan
AU  - Chen X
AD  - Department of Cardiology, The First Affiliated Hospital of Nanjing Medical 
      University Nanjing 210029, Jiangsu Province, P. R. China.
FAU - Li, Peng
AU  - Li P
AD  - Department of Cardiology, The First Affiliated Hospital of Nanjing Medical 
      University Nanjing 210029, Jiangsu Province, P. R. China.
FAU - Xiao, Qianxuan
AU  - Xiao Q
AD  - Department of Stomatology, Nanjing Medical University Nanjing 211166, Jiangsu 
      Province, P. R. China.
FAU - Hou, Daorong
AU  - Hou D
AD  - Key Laboratory of Model Animal Research, Animal Core Facility of Nanjing Medical 
      University, Nanjing Medical University Nanjing 211166, Jiangsu Province, P. R. 
      China.
FAU - Kong, Xiangqing
AU  - Kong X
AD  - Department of Cardiology, The First Affiliated Hospital of Nanjing Medical 
      University Nanjing 210029, Jiangsu Province, P. R. China.
LA  - eng
PT  - Journal Article
DEP - 20200915
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC7540123
OTO - NOTNLM
OT  - CD47 antibody
OT  - Cardiac hypertrophy
OT  - apoptosis
OT  - autophagy
OT  - oxidative stress
COIS- None.
EDAT- 2020/10/13 06:00
MHDA- 2020/10/13 06:01
PMCR- 2020/09/15
CRDT- 2020/10/12 05:30
PHST- 2019/11/04 00:00 [received]
PHST- 2020/06/02 00:00 [accepted]
PHST- 2020/10/12 05:30 [entrez]
PHST- 2020/10/13 06:00 [pubmed]
PHST- 2020/10/13 06:01 [medline]
PHST- 2020/09/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2020 Sep 15;12(9):5908-5923. eCollection 2020.