PMID- 33045298
OWN - NLM
STAT- MEDLINE
DCOM- 20210408
LR  - 20210408
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 165
IP  - Pt A
DP  - 2020 Dec 15
TI  - Molecular characterization, expression and functional analysis of TGFbeta1-b in 
      crucian carp (Carassius carassius).
PG  - 1392-1401
LID - S0141-8130(20)34645-6 [pii]
LID - 10.1016/j.ijbiomac.2020.10.024 [doi]
AB  - Transforming growth factor beta1 (TGFbeta1) is a polyfunctional cytokine with important 
      roles in growth, differentiation and immune function in various animals. In this 
      study, PCR, bioinformatics, real-time quantitative PCR, prokaryotic expression, 
      protein purification and matrix-assisted laser desorption/ionization 
      time-of-flight mass spectrometry (MALDI-TOF-TOF-MS) were applied to investigate 
      the structural features and function of TGFbeta1-b in crucian carp. The complete 
      coding sequence (CDS) of TGFbeta1-b was 1134 bp in length and was submitted to 
      GenBank (ID: MH473141). TGFbeta1-b encoded a putative protein of 377 amino acids and 
      included a signal peptide consisting of 22 amino acids. TGFbeta1-b was relatively 
      conservative in fish and distant from mammals in terms of evolutionary 
      relationship. TGFbeta1-b was found to be expressed in various tissues, with the 
      highest expression in the kidney. The expressions of TGFbeta1-b in muscle, heart and 
      liver were increased with the addition of Rhodopseudomonas palustris, Bacillus 
      subtilis and Enterococcus faecium at 30 days (p < 0.01). While, the expressions 
      of SMAD2, SMAD3 and SMAD7 were also up-regulated with the addition of R. 
      palustris at 20 days (p < 0.01). The expression of TGFbeta1-b could be affected by 
      time and group factors (p < 0.05). Moreover, the expression vector TGFbeta1-b-pDE2 
      was successfully constructed. Prokaryotic expression indicated that a 43 kDa 
      target protein was obtained after induction with 1.5 mM 
      isopropyl-beta-D-thiogalactopyranoside (IPTG) for 3.5 h at 37  degrees C for 200 r/h. The 
      activities of alkaline phosphatase and lysozyme in injection TGFbeta1-b protein 
      group (ITg) and feeding broken bacterial liquid group (BTg) were significantly 
      increased at 24 h (p < 0.01). And the activities of superoxide dismutase in ITg 
      were significantly increased at 36 h (p < 0.01). Besides, the expressions of heat 
      shock protein 30 and heat shock protein 47 in ITg and BTg were significantly 
      increased (p < 0.01). Whereas, the expression of interleukin-11 was significantly 
      reduced (p < 0.01). These results indicated that TGFbeta1-b protein might play a 
      role in immunity of crucian carp.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Li, Chenyang
AU  - Li C
AD  - Key Laboratory of Qinghai-Tibet Plateau Animal Genetic Resource Reservation and 
      Utilization, Ministry of Education and Sichuan Province, Southwest Minzu 
      University, Chendu 610041, PR China; Healthy Aquaculture Key Laboratory of 
      Sichuan Province, Chendu 610041, PR China.
FAU - Wang, Li
AU  - Wang L
AD  - Key Laboratory of Qinghai-Tibet Plateau Animal Genetic Resource Reservation and 
      Utilization, Ministry of Education and Sichuan Province, Southwest Minzu 
      University, Chendu 610041, PR China. Electronic address: qinxin916@aliyun.com.
LA  - eng
PT  - Journal Article
DEP - 20201010
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Amino Acid Sequence/genetics
MH  - Animals
MH  - Carps/*genetics
MH  - Cloning, Molecular
MH  - Gene Expression Regulation/genetics
MH  - Immunity, Innate/genetics
MH  - *Phylogeny
MH  - Sequence Alignment
MH  - Transforming Growth Factor beta1/chemistry/*genetics
OTO - NOTNLM
OT  - Crucian carp
OT  - Expression
OT  - Immunity
OT  - Probiotics
OT  - TGFbeta1-b
COIS- Declaration of competing interest No conflict of interest exits in the submission 
      of this manuscript, and manuscript is approved by all authors for publication.
EDAT- 2020/10/13 06:00
MHDA- 2021/04/10 06:00
CRDT- 2020/10/12 20:08
PHST- 2020/07/02 00:00 [received]
PHST- 2020/10/02 00:00 [revised]
PHST- 2020/10/03 00:00 [accepted]
PHST- 2020/10/13 06:00 [pubmed]
PHST- 2021/04/10 06:00 [medline]
PHST- 2020/10/12 20:08 [entrez]
AID - S0141-8130(20)34645-6 [pii]
AID - 10.1016/j.ijbiomac.2020.10.024 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2020 Dec 15;165(Pt A):1392-1401. doi: 
      10.1016/j.ijbiomac.2020.10.024. Epub 2020 Oct 10.