PMID- 33045641
OWN - NLM
STAT- MEDLINE
DCOM- 20210917
LR  - 20210917
IS  - 1873-5967 (Electronic)
IS  - 1386-6532 (Linking)
VI  - 132
DP  - 2020 Nov
TI  - Inherited chromosomally integrated human herpesvirus 6 and autoimmune connective 
      tissue diseases.
PG  - 104656
LID - S1386-6532(20)30398-X [pii]
LID - 10.1016/j.jcv.2020.104656 [doi]
AB  - BACKGROUND: Entire genome of human herpesvirus 6 (HHV-6) that integrates into 
      human chromosomes is called chromosomally integrated HHV-6 (ciHHV-6). Several 
      viral infections have been suggested to be involved in autoimmune connective 
      tissue diseases (CTDs). Reactivated HHV-6 from the integrated viral genome can 
      induce immune responses against the virus. Thus, it is plausible that ciHHV-6 is 
      associated with autoimmune CTDs. OBJECTIVES: We sought to determine whether the 
      prevalence of ciHHV-6 was significantly higher in patients with autoimmune CTDs 
      than in a healthy population. STUDY DESIGN: A total of 846 peripheral blood 
      samples collected from autoimmune CTD patients were analyzed. Since there was a 
      large number of samples, they were pooled into 24 samples per group. Copy numbers 
      of HHV-6 DNA were measured by real-time PCR. The threshold level for 
      distinguishing between ciHHV-6 and active viral infection and the reliability of 
      pooled DNA analysis were examined as initial validation experiments. RESULTS: The 
      threshold level was 1.6 x 10;6 copy/mL in whole blood. The reliability of pooled 
      DNA analysis to identify one ciHHV-6 sample among 23 HHV-6 DNA-negative samples 
      was high. No HHV-6 DNA was detected in any of the pooled DNA samples collected 
      from the patients. The probability of the present study including the 846 
      autoimmune CTD patient's samples was statistically not different with a healthy 
      Japanese population which was 0.2 % or 0.6 %. CONCLUSIONS: There was no 
      significant difference in the prevalence of ciHHV-6 between a healthy population 
      and patients with autoimmune CTDs.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Kawamura, Yoshiki
AU  - Kawamura Y
AD  - Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, 
      Aichi, Japan. Electronic address: yoshiki@fujita-fujita.ac.jp.
FAU - Hashimoto, Takako
AU  - Hashimoto T
AD  - Division of Rheumatology, Department of Internal Medicine, Fujita Health 
      University School of Medicine, Toyoake, Aichi, Japan.
FAU - Miura, Hiroki
AU  - Miura H
AD  - Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, 
      Aichi, Japan.
FAU - Kozawa, Kei
AU  - Kozawa K
AD  - Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, 
      Aichi, Japan.
FAU - Yoshikawa, Akiko
AU  - Yoshikawa A
AD  - Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, 
      Aichi, Japan.
FAU - Ikeda, Naomi
AU  - Ikeda N
AD  - Division of Rheumatology, Department of Internal Medicine, Fujita Health 
      University School of Medicine, Toyoake, Aichi, Japan.
FAU - Yatsuya, Hiroshi
AU  - Yatsuya H
AD  - Department of Public Health, Fujita Health University School of Medicine, 
      Toyoake, Aichi, Japan.
FAU - Yasuoka, Hidekata
AU  - Yasuoka H
AD  - Division of Rheumatology, Department of Internal Medicine, Fujita Health 
      University School of Medicine, Toyoake, Aichi, Japan.
FAU - Yoshikawa, Tetsushi
AU  - Yoshikawa T
AD  - Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, 
      Aichi, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201005
PL  - Netherlands
TA  - J Clin Virol
JT  - Journal of clinical virology : the official publication of the Pan American 
      Society for Clinical Virology
JID - 9815671
RN  - 0 (DNA, Viral)
SB  - IM
MH  - *Connective Tissue Diseases/genetics
MH  - DNA, Viral/genetics
MH  - *Herpesvirus 6, Human/genetics
MH  - Humans
MH  - Reproducibility of Results
MH  - *Roseolovirus Infections/epidemiology
MH  - Virus Integration
OTO - NOTNLM
OT  - Autoimmune connective tissue disease
OT  - Chromosomally integrated human herpesvirus 6
OT  - Human herpesvirus 6
EDAT- 2020/10/13 06:00
MHDA- 2021/09/18 06:00
CRDT- 2020/10/12 20:14
PHST- 2020/06/15 00:00 [received]
PHST- 2020/09/27 00:00 [revised]
PHST- 2020/10/03 00:00 [accepted]
PHST- 2020/10/13 06:00 [pubmed]
PHST- 2021/09/18 06:00 [medline]
PHST- 2020/10/12 20:14 [entrez]
AID - S1386-6532(20)30398-X [pii]
AID - 10.1016/j.jcv.2020.104656 [doi]
PST - ppublish
SO  - J Clin Virol. 2020 Nov;132:104656. doi: 10.1016/j.jcv.2020.104656. Epub 2020 Oct 
      5.