PMID- 33049389
OWN - NLM
STAT- MEDLINE
DCOM- 20210830
LR  - 20210830
IS  - 1769-714X (Electronic)
IS  - 1286-4579 (Linking)
VI  - 23
IP  - 1
DP  - 2021 Jan-Feb
TI  - TREM-1 enhances Mycobacterium tuberculosis-induced inflammatory responses in 
      macrophages.
PG  - 104765
LID - S1286-4579(20)30179-9 [pii]
LID - 10.1016/j.micinf.2020.10.001 [doi]
AB  - Triggering receptor expressed on myeloid cells 1 (TREM-1) extensively interacts 
      with toll-like receptors and amplifies pro-inflammatory responses. The effect of 
      TREM-1 on Mycobacterium tuberculosis (MTB)-related immune responses remains to be 
      elucidated. We isolated bone marrow-derived macrophages (BMDMs) from wild-type 
      mice and Trem-1 KO mice and treated them with MTB whole cell lysate and EsxA 
      (ESAT-6). Cytokine production and mRNA expression, including Trem-1, following 
      stimulation were evaluated. Intratracheal instillation of heat-killed MTB (HKMTB) 
      in mice was performed and the presence of TREM-1-positive macrophages was 
      investigated by immunohistochemistry analysis. In our study, BMDMs isolated from 
      wild-type mice produced more pro-inflammatory cytokines and demonstrated higher 
      inflammatory gene expression levels compared with those isolated from Trem-1 KO 
      mice when stimulated with MTB whole cell lysate. EsxA had a synergistic effect 
      with MTB whole cell lysate on the induction of pro-inflammatory responses. The 
      gene expression of Trem-1 was upregulated when treated with MTB-related proteins. 
      TREM-1-positive macrophages were identified in the lung tissues from patients 
      with active TB and from wild-type mice treated with intratracheal instillation of 
      HKMTB. In conclusion, in mouse macrophages, TREM-1 could enhance pro-inflammatory 
      immune responses when stimulated with MTB-related proteins. The gene expression 
      of Trem-1 could also be induced by MTB-related stimulation.
CI  - Copyright (c) 2020. Published by Elsevier Masson SAS.
FAU - Feng, Jia-Yih
AU  - Feng JY
AD  - Department of Chest Medicine, Taipei Veterans General Hospital, Taipei, Taiwan; 
      School of Medicine, National Yang-Ming University, Taipei, Taiwan; Institute of 
      Clinical Medicine, National Yang-Ming University, Taipei, Taiwan.
FAU - Su, Wei-Juin
AU  - Su WJ
AD  - Department of Chest Medicine, Taipei Veterans General Hospital, Taipei, Taiwan; 
      School of Medicine, National Yang-Ming University, Taipei, Taiwan.
FAU - Chuang, Fan-Yi
AU  - Chuang FY
AD  - Department of Chest Medicine, Taipei Veterans General Hospital, Taipei, Taiwan.
FAU - Pan, Sheng-Wei
AU  - Pan SW
AD  - Department of Chest Medicine, Taipei Veterans General Hospital, Taipei, Taiwan; 
      School of Medicine, National Yang-Ming University, Taipei, Taiwan; Institute of 
      Public Health, National Yang-Ming University, Taipei, Taiwan.
FAU - Yeh, Yi-Chen
AU  - Yeh YC
AD  - Department of Pathology and Laboratory Medicine, Taipei Veterans General 
      Hospital, Taipei, Taiwan.
FAU - Lin, Yung-Yang
AU  - Lin YY
AD  - Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan; 
      Division of Cerebrovascular Diseases, Neurological Institute, Taipei Veterans 
      General Hospital, Taipei, Taiwan; Institute of Brain Science, National Yang-Ming 
      University, Taipei, Taiwan.
FAU - Chen, Nien-Jung
AU  - Chen NJ
AD  - Institute of Microbiology and Immunology, School of Life Sciences, National 
      Yang-Ming University, Taipei, Taiwan. Electronic address: njchen@ym.edu.tw.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201010
PL  - France
TA  - Microbes Infect
JT  - Microbes and infection
JID - 100883508
RN  - 0 (Triggering Receptor Expressed on Myeloid Cells-1)
SB  - IM
MH  - Animals
MH  - Female
MH  - Host-Pathogen Interactions
MH  - Humans
MH  - Macrophages/*immunology/microbiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Mycobacterium tuberculosis/genetics/*immunology/metabolism
MH  - Triggering Receptor Expressed on Myeloid Cells-1/genetics/*immunology
MH  - Tuberculosis/genetics/*immunology/*microbiology
OTO - NOTNLM
OT  - EsxA (ESAT-6)
OT  - Innate immunity
OT  - Macrophages
OT  - Triggering receptor expressed on myeloid cells-1
OT  - Tuberculosis
COIS- Declaration of competing interest The authors declare that they have no competing 
      interests.
EDAT- 2020/10/14 06:00
MHDA- 2021/08/31 06:00
CRDT- 2020/10/13 20:09
PHST- 2019/06/05 00:00 [received]
PHST- 2020/08/09 00:00 [revised]
PHST- 2020/10/05 00:00 [accepted]
PHST- 2020/10/14 06:00 [pubmed]
PHST- 2021/08/31 06:00 [medline]
PHST- 2020/10/13 20:09 [entrez]
AID - S1286-4579(20)30179-9 [pii]
AID - 10.1016/j.micinf.2020.10.001 [doi]
PST - ppublish
SO  - Microbes Infect. 2021 Jan-Feb;23(1):104765. doi: 10.1016/j.micinf.2020.10.001. 
      Epub 2020 Oct 10.