PMID- 33049560
OWN - NLM
STAT- MEDLINE
DCOM- 20210125
LR  - 20210125
IS  - 1872-9142 (Electronic)
IS  - 0161-5890 (Linking)
VI  - 128
DP  - 2020 Dec
TI  - Helminth-derived peptide GK-1 induces Myd88-dependent pro-inflammatory signaling 
      events in bone marrow-derived antigen-presenting cells.
PG  - 22-32
LID - S0161-5890(20)30491-0 [pii]
LID - 10.1016/j.molimm.2020.09.015 [doi]
AB  - GK-1 is an immunomodulatory, 18-aa-long peptide that has been proved to promote 
      the activation of mouse peritoneal macrophages and LPS-pulsed mouse bone 
      marrow-derived dendritic cells (BM-DCs). This study is aimed to explore the 
      mechanisms underlying the activation of these antigen-presenting cells (APCs) by 
      GK-1. In our study, GK-1 up-regulated in vitro the expression of CD86 and CD40, 
      and it increased the secretion of NO in bone marrow-derived macrophages (BMDMs). 
      In BM-DCs, GK-1 upregulated the expression of MHC class II and CD86. 
      Additionally, GK-1 was found to be involved in the phosphorylation of MAPK p38, 
      JNK and ERK 1/2 and in Myd88-dependent activation of NF-kappaB in both 
      antigen-presenting cell types. In vivo, GK-1 increased the secretion of IL-15, 
      CCL2, and IL-6 through a Myd88-dependent mechanism. This study demonstrated that 
      GK-1 promotes the activation and effector activity of APCs through a mechanism 
      dependent on Myd88, probably involving a Toll-like receptor as a target.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Montero, Laura
AU  - Montero L
AD  - Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, 
      Mexico City, 04510, Mexico. Electronic address: lau_mlcrl@yahoo.com.
FAU - Cervantes-Torres, Jacquelynne
AU  - Cervantes-Torres J
AD  - Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, 
      Mexico City, 04510, Mexico. Electronic address: jcervantes@iibiomedicas.unam.mx.
FAU - Sciutto, Edda
AU  - Sciutto E
AD  - Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, 
      Mexico City, 04510, Mexico. Electronic address: edda@unam.mx.
FAU - Fragoso, Gladis
AU  - Fragoso G
AD  - Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, 
      Mexico City, 04510, Mexico. Electronic address: gladis@unam.mx.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201010
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD40 Antigens)
RN  - 0 (Cytokines)
RN  - 0 (Helminth Proteins)
RN  - 0 (Myd88 protein, mouse)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Peptides, Cyclic)
RN  - 0 (cyclo(cysteinyl-glycyl-aspargyl-seryl-aspargyl-prolyl-lysyl-seryl-cysteine))
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Antigen-Presenting Cells/*metabolism
MH  - B7-2 Antigen/metabolism
MH  - Bone Marrow/*metabolism
MH  - Bone Marrow Cells/metabolism
MH  - CD40 Antigens/metabolism
MH  - Cells, Cultured
MH  - Cytokines
MH  - Dendritic Cells/metabolism
MH  - Female
MH  - Helminth Proteins/*metabolism
MH  - Helminths/*metabolism
MH  - Inflammation/*metabolism
MH  - Macrophages, Peritoneal/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Myeloid Differentiation Factor 88/*metabolism
MH  - Peptides, Cyclic/*metabolism
MH  - Phosphorylation/physiology
MH  - Signal Transduction/physiology
OTO - NOTNLM
OT  - Dendritic cells
OT  - GK-1 peptide
OT  - MAPKs
OT  - Macrophages
OT  - NF-kappaB
EDAT- 2020/10/14 06:00
MHDA- 2021/01/26 06:00
CRDT- 2020/10/13 20:14
PHST- 2020/02/18 00:00 [received]
PHST- 2020/09/19 00:00 [revised]
PHST- 2020/09/21 00:00 [accepted]
PHST- 2020/10/14 06:00 [pubmed]
PHST- 2021/01/26 06:00 [medline]
PHST- 2020/10/13 20:14 [entrez]
AID - S0161-5890(20)30491-0 [pii]
AID - 10.1016/j.molimm.2020.09.015 [doi]
PST - ppublish
SO  - Mol Immunol. 2020 Dec;128:22-32. doi: 10.1016/j.molimm.2020.09.015. Epub 2020 Oct 
      10.