PMID- 33050354
OWN - NLM
STAT- MEDLINE
DCOM- 20210226
LR  - 20210226
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 21
IP  - 20
DP  - 2020 Oct 9
TI  - The Antioxidant Capacity In Vitro and In Vivo of Polysaccharides From Bergenia 
      emeiensis.
LID - 10.3390/ijms21207456 [doi]
LID - 7456
AB  - Polysaccharides from Bergenia emeiensis (PBE) showed a robust antioxidant ability 
      on scavenging free radicals in vitro. However, the further antioxidant potential 
      in cell level and in vivo was still unknown. Therefore, in this present study, 
      the protective effect of PBE on human cervical carcinoma cell (Hela) cells and 
      Caenorhabditis elegans against oxidative stress was evaluated. The results showed 
      PBE could reduce the reactive oxygen species (ROS) level in Hela cells and 
      promote the mitochondrial membrane potential. Then, the cell apoptosis was 
      reduced. Moreover, PBE could enhance the survival of C. elegans under thermal 
      stress to 13.44%, and significantly reduce the ROS level, which was connected 
      with the overexpression of sod-3 and the increased nuclear localization of daf-16 
      transcription factor. Therefore, PBE exhibited a strong antioxidant capacity in 
      the cellular level and for a whole organism. Thus, polysaccharides from B. 
      emeiensis have natural potential to be a safe antioxidant.
FAU - Zeng, Chen
AU  - Zeng C
AD  - College of Life Science, Sichuan Agricultural University, Ya'an 625014, China.
FAU - Feng, Shiling
AU  - Feng S
AD  - College of Life Science, Sichuan Agricultural University, Ya'an 625014, China.
LA  - eng
GR  - 201910626047/The Innovation Training Program of Sichuan Agricultural University/
PT  - Journal Article
DEP - 20201009
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Antioxidants)
RN  - 0 (Plant Extracts)
RN  - 0 (Polysaccharides)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Antioxidants/chemistry/*pharmacology
MH  - Apoptosis/drug effects
MH  - Caenorhabditis elegans/drug effects/metabolism
MH  - Cell Survival/drug effects
MH  - Gene Expression Regulation
MH  - Oxidative Stress/drug effects
MH  - Plant Extracts/chemistry/isolation & purification/*pharmacology
MH  - Polysaccharides/chemistry/isolation & purification/*pharmacology
MH  - Reactive Oxygen Species/metabolism
MH  - Saxifragaceae/*chemistry
MH  - Transcription Factors/metabolism
PMC - PMC7589108
OTO - NOTNLM
OT  - Bergenia emeiensis
OT  - C. elegans
OT  - antioxidant ability
OT  - polysaccharides
COIS- The authors declare no conflict of interest.
EDAT- 2020/10/15 06:00
MHDA- 2021/02/27 06:00
PMCR- 2020/10/01
CRDT- 2020/10/14 01:04
PHST- 2020/09/23 00:00 [received]
PHST- 2020/10/07 00:00 [revised]
PHST- 2020/10/07 00:00 [accepted]
PHST- 2020/10/14 01:04 [entrez]
PHST- 2020/10/15 06:00 [pubmed]
PHST- 2021/02/27 06:00 [medline]
PHST- 2020/10/01 00:00 [pmc-release]
AID - ijms21207456 [pii]
AID - ijms-21-07456 [pii]
AID - 10.3390/ijms21207456 [doi]
PST - epublish
SO  - Int J Mol Sci. 2020 Oct 9;21(20):7456. doi: 10.3390/ijms21207456.