PMID- 33050411
OWN - NLM
STAT- MEDLINE
DCOM- 20210715
LR  - 20210715
IS  - 2073-4425 (Electronic)
IS  - 2073-4425 (Linking)
VI  - 11
IP  - 10
DP  - 2020 Oct 10
TI  - Multiple Sex Chromosomes and Evolutionary Relationships in Amazonian Catfishes: 
      The Outstanding Model of the Genus Harttia (Siluriformes: Loricariidae).
LID - 10.3390/genes11101179 [doi]
LID - 1179
AB  - The armored Harttia catfishes present great species diversity and remarkable 
      cytogenetic variation, including different sex chromosome systems. Here we 
      analyzed three new species, H. duriventris, H. villasboas and H. rondoni, using 
      both conventional and molecular cytogenetic techniques (Giemsa-staining and 
      C-banding), including the mapping of repetitive DNAs using fluorescence in situ 
      hybridization (FISH) and comparative genomic hybridization (CGH) experiments. 
      Both H. duriventris and H. villasboas have 2n = female symbol56/male symbol55 chromosomes, and an 
      X(1)X(1)X(2)X(2) /X(1)X(2)Y sex chromosome system, while a proto or neo-XY system 
      is proposed for H. rondoni (2n = 54female symbolmale symbol). Single motifs of 5S and 18S rDNA occur in 
      all three species, with the latter being also mapped in the sex chromosomes. The 
      results confirm the general evolutionary trend that has been noticed for the 
      genus: an extensive variation on their chromosome number, single sites of rDNA 
      sequences and the occurrence of multiple sex chromosomes. Comparative genomic 
      analyses with another congeneric species, H. punctata, reveal that the X(1)X(2)Y 
      sex chromosomes of these species share the genomic contents, indicating a 
      probable common origin. The remarkable karyotypic variation, including sex 
      chromosomes systems, makes Harttia a suitable model for evolutionary studies 
      focusing on karyotype differentiation and sex chromosome evolution among lower 
      vertebrates.
FAU - Sassi, Francisco de M C
AU  - Sassi FMC
AUID- ORCID: 0000-0001-5699-5789
AD  - Laboratorio de Citogenetica de Peixes, Departamento de Genetica e Evolucao, 
      Universidade Federal de Sao, Carlos, Sao Carlos - SP 13565-905, Brazil.
FAU - Deon, Geize A
AU  - Deon GA
AD  - Laboratorio de Citogenetica de Peixes, Departamento de Genetica e Evolucao, 
      Universidade Federal de Sao, Carlos, Sao Carlos - SP 13565-905, Brazil.
AD  - Departamento de Biologia Estrutural, Molecular e Genetica, Universidade Estadual 
      de Ponta Grossa, Ponta Grossa - PR 84010-330, Brazil.
FAU - Moreira-Filho, Orlando
AU  - Moreira-Filho O
AD  - Laboratorio de Citogenetica de Peixes, Departamento de Genetica e Evolucao, 
      Universidade Federal de Sao, Carlos, Sao Carlos - SP 13565-905, Brazil.
FAU - Vicari, Marcelo R
AU  - Vicari MR
AD  - Departamento de Biologia Estrutural, Molecular e Genetica, Universidade Estadual 
      de Ponta Grossa, Ponta Grossa - PR 84010-330, Brazil.
FAU - Bertollo, Luiz A C
AU  - Bertollo LAC
AD  - Laboratorio de Citogenetica de Peixes, Departamento de Genetica e Evolucao, 
      Universidade Federal de Sao, Carlos, Sao Carlos - SP 13565-905, Brazil.
FAU - Liehr, Thomas
AU  - Liehr T
AUID- ORCID: 0000-0003-1672-3054
AD  - Institute of Human Genetics, University Hospital Jena, 07747 Jena, Germany.
FAU - Oliveira, Ezequiel Aguiar de
AU  - Oliveira EA
AUID- ORCID: 0000-0002-8264-8884
AD  - Secretaria de Estado de Educacao do Mato Grosso-SEDUC-MT, Cuiaba, MT 78049-909, 
      Brazil.
FAU - Cioffi, Marcelo B
AU  - Cioffi MB
AUID- ORCID: 0000-0003-4340-1464
AD  - Laboratorio de Citogenetica de Peixes, Departamento de Genetica e Evolucao, 
      Universidade Federal de Sao, Carlos, Sao Carlos - SP 13565-905, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201010
PL  - Switzerland
TA  - Genes (Basel)
JT  - Genes
JID - 101551097
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Animals
MH  - Catfishes/*genetics
MH  - DNA, Ribosomal/analysis/genetics
MH  - *Evolution, Molecular
MH  - *Genetic Variation
MH  - *Genome
MH  - Genomics
MH  - Karyotyping
MH  - Sex Chromosomes/*genetics
PMC - PMC7600804
OTO - NOTNLM
OT  - Cytogenetics
OT  - Neotropical fishes
OT  - comparative genomic hybridization
OT  - ribosomal DNA
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2020/10/15 06:00
MHDA- 2021/07/16 06:00
PMCR- 2020/10/01
CRDT- 2020/10/14 01:04
PHST- 2020/09/14 00:00 [received]
PHST- 2020/10/06 00:00 [revised]
PHST- 2020/10/08 00:00 [accepted]
PHST- 2020/10/14 01:04 [entrez]
PHST- 2020/10/15 06:00 [pubmed]
PHST- 2021/07/16 06:00 [medline]
PHST- 2020/10/01 00:00 [pmc-release]
AID - genes11101179 [pii]
AID - genes-11-01179 [pii]
AID - 10.3390/genes11101179 [doi]
PST - epublish
SO  - Genes (Basel). 2020 Oct 10;11(10):1179. doi: 10.3390/genes11101179.