PMID- 33050458
OWN - NLM
STAT- MEDLINE
DCOM- 20210517
LR  - 20210517
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 25
IP  - 20
DP  - 2020 Oct 10
TI  - The Chemical Composition of Brazilian Green Propolis and Its Protective Effects 
      on Mouse Aortic Endothelial Cells against Inflammatory Injury.
LID - 10.3390/molecules25204612 [doi]
LID - 4612
AB  - Propolis has a very complex composition, with antibacterial, anti-inflammatory 
      and other properties. To determine the composition of ethanol extracts of 
      Brazilian green propolis (EEP-B) and their protective effect on mouse aortic 
      endothelial cells (MAECs), the chemical composition of EEP-B was analysed by 
      UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of 
      lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 
      (CCK-8) assays. The protein levels of inflammatory cytokines tumour necrosis 
      factor-alpha (TNF-alpha) and interleukin- 6 (IL-6) were measured by enzyme-linked 
      immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were 
      analysed by western blotting. The results showed that a total of 24 compounds 
      belonging to cinnamic acids and flavonoids, including 
      3,5-diisopentenyl-4-hydroxycinnamic acid (artepillin C), kaempferide, 3-isoprenyl 
      p-coumaric acid, pinocembrin and 4'-methoxy pinobanksin, were identified in 
      EEP-B. Among them, a new component, suggested to be 5-isoprenyl caffeic acid 
      p-coumaric acid ester, was reported for the first time. The LPS-induced levels of 
      TNF-alpha, IL-6, ICAM-1, VCAM-1 and MCP-1 were downregulated in response to 5, 10 and 
      20 mug/mL EEP-B. This study revealed that EEP-B could reduce LPS-induced 
      inflammatory reactions, improve cell survival, and protect MAECs by regulating 
      ICAM-1, VCAM-1 and MCP-1 expression. These findings could provide a theoretical 
      basis for MAEC treatment using EEP-B.
FAU - Xu, Xiaolan
AU  - Xu X
AD  - College of Animal Science (College of Bee Science), Fujian Agriculture and 
      Forestry University, Fuzhou 350002, China.
FAU - Yang, Bo
AU  - Yang B
AD  - College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 
      350002, China.
AD  - Bee Product Processing and Application Research Center of the Ministry of 
      Education, Fuzhou 350002, China.
FAU - Wang, Danfeng
AU  - Wang D
AD  - College of Plant Protection, Institute of Applied Ecology, Fujian Agriculture and 
      Forestry University, Fuzhou 350002, China.
FAU - Zhu, Yuxuan
AU  - Zhu Y
AD  - College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 
      350002, China.
AD  - Bee Product Processing and Application Research Center of the Ministry of 
      Education, Fuzhou 350002, China.
FAU - Miao, Xiaoqing
AU  - Miao X
AD  - Bee Product Processing and Application Research Center of the Ministry of 
      Education, Fuzhou 350002, China.
FAU - Yang, Wenchao
AU  - Yang W
AUID- ORCID: 0000-0003-2512-5363
AD  - College of Animal Science (College of Bee Science), Fujian Agriculture and 
      Forestry University, Fuzhou 350002, China.
AD  - Bee Product Processing and Application Research Center of the Ministry of 
      Education, Fuzhou 350002, China.
LA  - eng
GR  - CARS-44-KXJ20/Modern Agro-industry Technology Research System/
PT  - Journal Article
DEP - 20201010
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 9009-62-5 (Propolis)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*chemistry/pharmacology
MH  - Chemokine CCL2/metabolism
MH  - Endothelial Cells/drug effects/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Interleukin-6/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Mice
MH  - Propolis/*chemistry
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Vascular Cell Adhesion Molecule-1/metabolism
PMC - PMC7587206
OTO - NOTNLM
OT  - Brazilian green propolis
OT  - anti-inflammatory
OT  - chemical composition
OT  - mouse aortic endothelial cells
COIS- The authors declare no conflict of interest. The founding sponsors had no role in 
      the design of the study; in the collection, analyses, or interpretation of data; 
      in the writing of the manuscript, and in the decision to publish the results.
EDAT- 2020/10/15 06:00
MHDA- 2021/05/18 06:00
PMCR- 2020/10/10
CRDT- 2020/10/14 01:04
PHST- 2020/09/05 00:00 [received]
PHST- 2020/09/28 00:00 [revised]
PHST- 2020/10/03 00:00 [accepted]
PHST- 2020/10/14 01:04 [entrez]
PHST- 2020/10/15 06:00 [pubmed]
PHST- 2021/05/18 06:00 [medline]
PHST- 2020/10/10 00:00 [pmc-release]
AID - molecules25204612 [pii]
AID - molecules-25-04612 [pii]
AID - 10.3390/molecules25204612 [doi]
PST - epublish
SO  - Molecules. 2020 Oct 10;25(20):4612. doi: 10.3390/molecules25204612.