PMID- 33051415
OWN - NLM
STAT- MEDLINE
DCOM- 20201015
LR  - 20220531
IS  - 1672-7347 (Print)
IS  - 1672-7347 (Linking)
VI  - 45
IP  - 9
DP  - 2020 Sept 28
TI  - Decreased expression of prohibitin 1 mediates high-mobility group protein 
      1-induced pulmonary artery endothelial cell injury.
PG  - 1024-1034
LID - 1672-7347(2020)09-1024-11 [pii]
LID - 10.11817/j.issn.1672-7347.2020.190150 [doi]
AB  - OBJECTIVES: There is a significant increase of high-mobility group protein B1 
      (HMGB1) in plasma levels of patients with pulmonary hypertension, but the 
      biological significance is still unclear. Anti-proliferative protein 1 
      (prohibitin 1, PHB1) is an important protein that maintains the homeostasis of 
      vascular cells. This study aimed to investigate the effect of HMGB1 on pulmonary 
      artery endothelial cells and the role of PHB1. METHODS: In vivo experiment: A rat 
      model of pulmonary hypertension induced by monocrotaline (MCT) was constructed. 
      The right ventricular systolic pressure (RVSP), and the weight ratio of right 
      ventricle to left ventricle plus ventricular septum were used to evaluate the 
      success of model. ELISA was used to detect the level of HMGB1 in rat's plasma. 
      Western blotting was used to detect the level of PHB1 in rat's lung tissues. CD31 
      immunofluorescence was used to detect the integrity of pulmonary vascular 
      endothelium. In vitro experiments: Pulmonary artery endothelial cell (PAEC) was 
      incubated with HMGB1 to observe the effect of HMGB1 on PAEC injury. 
      Overexpression and knockdown of PHB1 were conducted, and the role of PHB1 was 
      investigated by detecting the levels of reative oxygen species and cytochrome c 
      (cyto-c), and the activation of caspase-3. RESULTS: Compared with the control 
      group, the level of HMGB1 in the plasma of rats with pulmonary hypertension was 
      significantly increased (P<0.05), and the expression of PHB1 in the lung tissue 
      was decreased accompanied with endothelial dysfunction (P<0.05); HMGB1 incubation 
      damaged the pulmonary artery endothelium and down-regulated PHB1 expression 
      (P<0.05), while overexpression of PHB1 reduced the PAEC damage and oxidative 
      stress induced by HMGB1 (P<0.05). Meanwhile, PHB1 reduced HMGB1-induced cyto-c 
      expression and caspase-3 cleavage by inhibiting oxidative stress (P<0.05). 
      CONCLUSIONS: The down-regulation of PHB1 expression mediates HMGB1-induced PAEC 
      injury, which is related to the induction of oxidative stress, the increase of 
      cyto-c release, and the promotion of caspase-3 cleavage.
FAU - Xu, Yizhou
AU  - Xu Y
AD  - Department of Cardiology, Third Xiangya Hospital, Central South University, 
      Changsha 410013. xyz-27366508@qq.com.
FAU - Ye, Chen
AU  - Ye C
AD  - Xiangya School of Pharmaceutical Science, Central South University, Changsha 
      410078.
FAU - Tang, Lei
AU  - Tang L
AD  - Xiangya School of Pharmaceutical Science, Central South University, Changsha 
      410078.
FAU - Pan, Bingjie
AU  - Pan B
AD  - Xiangya School of Pharmaceutical Science, Central South University, Changsha 
      410078.
FAU - Li, Ying
AU  - Li Y
AD  - Department of Health Management, Third Xiangya Hospital, Central South 
      University, Changsha 410013, China. lydia0312@csu.edu.cn.
LA  - eng
LA  - chi
GR  - JY201709/the "New Xiangya Talent Project" of the Third Xiangya Hospital, Cenctral 
      South University, China/
PT  - Journal Article
TT  - PHB1表达下调介导HMGB1促肺动脉内皮细胞损伤.
PL  - China
TA  - Zhong Nan Da Xue Xue Bao Yi Xue Ban
JT  - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. 
      Medical sciences
JID - 101230586
RN  - 0 (HMGB1 Protein)
RN  - 0 (PHB protein, human)
RN  - 0 (Prohibitins)
RN  - 0 (Repressor Proteins)
SB  - IM
MH  - Animals
MH  - Endothelial Cells
MH  - *HMGB1 Protein/genetics
MH  - Humans
MH  - Prohibitins
MH  - Pulmonary Artery
MH  - Rats
MH  - *Repressor Proteins/genetics
OTO - NOTNLM
OT  - high-mobility group protein 1
OT  - prohibitin
OT  - pulmonary artery endothelial cells
OT  - pulmonary artery hypertension
EDAT- 2020/10/15 06:00
MHDA- 2020/10/21 06:00
CRDT- 2020/10/14 09:13
PHST- 2020/10/14 09:13 [entrez]
PHST- 2020/10/15 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
AID - 1672-7347(2020)09-1024-11 [pii]
AID - 10.11817/j.issn.1672-7347.2020.190150 [doi]
PST - ppublish
SO  - Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2020 Sept 28;45(9):1024-1034. doi: 
      10.11817/j.issn.1672-7347.2020.190150.