PMID- 33051512
OWN - NLM
STAT- MEDLINE
DCOM- 20210113
LR  - 20210113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Oct 13
TI  - A novel microfluidic chip-based sperm-sorting device constructed using design of 
      experiment method.
PG  - 17143
LID - 10.1038/s41598-020-73841-3 [doi]
LID - 17143
AB  - Microfluidics is proposed as a technique for efficient sperm sorting, to achieve 
      the ultimate goal of resolving infertility problems in livestock industry. Our 
      study aimed to design a microfluidic sperm-sorting device (SSD) through a 
      high-efficacy and cost- and time-effective fabrication process, by using COMSOL 
      Multiphysics simulation and modeling software, and the design of experiment (DOE) 
      method. The eight factors affecting SSD performance were established. The 
      simulation was then run, and statistically significant factors were analyzed. 
      Minitab16 was used to optimize the design modulus factor. By setting the 
      statistical significance at p < 0.05, the factors affecting experimental 
      structure were analyzed. At a desirability of 97.99, the optimal parameters for 
      the microfluidic chip were: angle between sperm and medium inlet chambers 
      (A = 43 degrees ), sperm inlet flow rate (B = 0.24 microL min(-1)), medium inlet flow rate 
      (C = 0.34 microL min(-1)), and inlet and outlet chamber lengths (D = 5000 microm). These 
      optima were then applied to microfluidics device construction. The device was 
      produced using soft lithographic microfabrication techniques and tested on 
      Holstein-Friesian bull sperm. The highest bull sperm-sorting performance for this 
      microfluidic device prototype was 96%. The error between the simulation and the 
      actual microfluidic device was 2.72%. Fluid viscosity ranges analysis-based 
      simulations revealed acceptable fluid viscosity tolerances for the SSD. The 
      simulation results revealed that the acceptable tolerance range for fluid 
      viscosity was 0.00001-0.003 kg m(-1) s(-1). This optimally designed microfluidic 
      chip-based SSD may be integrated into sperm x/y separation micro devices.
FAU - Phiphattanaphiphop, Chalinee
AU  - Phiphattanaphiphop C
AD  - Degree Program in Industrial Engineering, Department of Industrial Engineering, 
      Faculty of Engineering, Chiang Mai University, Chiang Mai, 50200, Thailand.
AD  - Industrial Engineering Department, Chiang Mai University, Chiang Mai, 50200, 
      Thailand.
FAU - Leksakul, Komgrit
AU  - Leksakul K
AD  - Industrial Engineering Department, Chiang Mai University, Chiang Mai, 50200, 
      Thailand. komgrit@eng.cmu.ac.th.
FAU - Phatthanakun, Rungrueang
AU  - Phatthanakun R
AD  - Synchrotron Light Research Institute (Public Organization), 111 University 
      Avenue, Nakhon Ratchasima, 30000, Thailand.
FAU - Khamlor, Trisadee
AU  - Khamlor T
AD  - Department of Animal and Aquatic Science, Faculty of Agriculture, Chiang Mai 
      University, Chiang Mai, 50200, Thailand.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201013
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Separation/*instrumentation
MH  - Equipment Design/*instrumentation
MH  - Infertility/therapy
MH  - Lab-On-A-Chip Devices
MH  - Livestock/physiology
MH  - Male
MH  - Microfluidic Analytical Techniques/instrumentation
MH  - Microfluidics/*instrumentation
MH  - Sperm Motility/physiology
MH  - Spermatozoa/cytology
PMC - PMC7553928
COIS- The authors declare no competing interests.
EDAT- 2020/10/15 06:00
MHDA- 2021/01/14 06:00
PMCR- 2020/10/13
CRDT- 2020/10/14 09:14
PHST- 2020/06/25 00:00 [received]
PHST- 2020/09/23 00:00 [accepted]
PHST- 2020/10/14 09:14 [entrez]
PHST- 2020/10/15 06:00 [pubmed]
PHST- 2021/01/14 06:00 [medline]
PHST- 2020/10/13 00:00 [pmc-release]
AID - 10.1038/s41598-020-73841-3 [pii]
AID - 73841 [pii]
AID - 10.1038/s41598-020-73841-3 [doi]
PST - epublish
SO  - Sci Rep. 2020 Oct 13;10(1):17143. doi: 10.1038/s41598-020-73841-3.