PMID- 33053109
OWN - NLM
STAT- MEDLINE
DCOM- 20201201
LR  - 20201201
IS  - 1414-431X (Electronic)
IS  - 0100-879X (Print)
IS  - 0100-879X (Linking)
VI  - 53
IP  - 11
DP  - 2020
TI  - Increased elastic modulus of the synovial membrane in a rat ACLT model of 
      osteoarthritis revealed by atomic force microscopy.
PG  - e10058
LID - S0100-879X2020001100608 [pii]
LID - 10.1590/1414-431X202010058 [doi]
LID - e10058
AB  - This study aimed to explore changes in nanoscale elastic modulus of the synovium 
      using atomic force microscopy (AFM) in addition to investigate changes in 
      synovial histomorphology and secretory function in osteoarthritis (OA) in a rat 
      anterior cruciate ligament transection (ACLT) model. Sprague-Dawley rats were 
      randomly assigned to sham control and ACLT OA groups. All right knee joints were 
      harvested at 4, 8, or 12 weeks (W) after surgery for histological assessment of 
      cartilage damage and synovitis in both the anterior and posterior capsules. AFM 
      imaging and nanoscale biomechanical testing were conducted to measure the elastic 
      modulus of the synovial collagen fibrils. Immunohistochemistry was used to 
      visualize the expression of interleukin-1beta (IL-1beta), tumor necrosis factor-alpha 
      (TNF-alpha), and matrix metalloproteinase-3 (MMP-3) in the synovium. The OA groups 
      exhibited progressive development of disease in the cartilage and synovium. 
      Histopathological scores of the synovium in the OA groups increased gradually. 
      Significant differences were observed between all OA groups except for the 
      posterior 4W group. The synovial fibril arrangement in all OA groups was 
      significantly disordered. The synovial fibrils in all ACLT OA groups at each time 
      point were stiffer than those in the sham controls. OA rats displayed a 
      significantly higher expression of IL-1beta and MMP3 in the anterior capsule. In 
      summary, synovial stiffening was closely associated with joint degeneration and 
      might be a factor contributing to synovitis and increased production of 
      proinflammatory mediators. Our data provided insights into the role of synovitis, 
      particularly stiffening of the synovium, in OA pathogenesis.
FAU - Dai, Shouqian
AU  - Dai S
AUID- ORCID: 0000-0001-8250-4541
AD  - Orthopedic Institute, Department of Orthopaedics, The First Affiliated Hospital 
      of Soochow University, Suzhou, Jiangsu, China.
AD  - Department of Emergency Medicine, The First Affiliated Hospital of Soochow 
      University, Suzhou, Jiangsu, China.
FAU - Liang, Ting
AU  - Liang T
AUID- ORCID: 0000-0001-7467-3046
AD  - Orthopedic Institute, Department of Orthopaedics, The First Affiliated Hospital 
      of Soochow University, Suzhou, Jiangsu, China.
FAU - Fujii, Tadashi
AU  - Fujii T
AUID- ORCID: 0000-0003-4743-2006
AD  - Department of Orthopaedic Surgery, Kashiba Asahigaoka Hospital, Kashiba, Nara, 
      Japan.
FAU - He, Shuangjun
AU  - He S
AUID- ORCID: 0000-0003-2023-0500
AD  - Orthopedic Institute, Department of Orthopaedics, The First Affiliated Hospital 
      of Soochow University, Suzhou, Jiangsu, China.
FAU - Zhang, Fan
AU  - Zhang F
AUID- ORCID: 0000-0002-2715-5657
AD  - Orthopedic Institute, Department of Orthopaedics, The First Affiliated Hospital 
      of Soochow University, Suzhou, Jiangsu, China.
FAU - Jiang, Huaye
AU  - Jiang H
AUID- ORCID: 0000-0002-9337-9528
AD  - Orthopedic Institute, Department of Orthopaedics, The First Affiliated Hospital 
      of Soochow University, Suzhou, Jiangsu, China.
FAU - Liu, Bo
AU  - Liu B
AUID- ORCID: 0000-0002-5811-1737
AD  - Orthopedic Institute, Department of Orthopaedics, The First Affiliated Hospital 
      of Soochow University, Suzhou, Jiangsu, China.
FAU - Shi, Xiu
AU  - Shi X
AUID- ORCID: 0000-0003-3163-2062
AD  - Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow 
      University, Suzhou, Jiangsu, China.
FAU - Luo, Zongping
AU  - Luo Z
AUID- ORCID: 0000-0002-0617-2597
AD  - Orthopedic Institute, Department of Orthopaedics, The First Affiliated Hospital 
      of Soochow University, Suzhou, Jiangsu, China.
FAU - Yang, Huilin
AU  - Yang H
AUID- ORCID: 0000-0001-8655-1513
AD  - Orthopedic Institute, Department of Orthopaedics, The First Affiliated Hospital 
      of Soochow University, Suzhou, Jiangsu, China.
LA  - eng
PT  - Journal Article
DEP - 20201007
PL  - Brazil
TA  - Braz J Med Biol Res
JT  - Brazilian journal of medical and biological research = Revista brasileira de 
      pesquisas medicas e biologicas
JID - 8112917
SB  - IM
MH  - Animals
MH  - Anterior Cruciate Ligament
MH  - *Cartilage, Articular
MH  - Elastic Modulus
MH  - Male
MH  - Microscopy, Atomic Force
MH  - *Osteoarthritis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Synovial Membrane
PMC - PMC7552902
EDAT- 2020/10/15 06:00
MHDA- 2020/12/02 06:00
PMCR- 2020/10/07
CRDT- 2020/10/14 18:00
PHST- 2020/03/23 00:00 [received]
PHST- 2020/07/22 00:00 [accepted]
PHST- 2020/10/14 18:00 [entrez]
PHST- 2020/10/15 06:00 [pubmed]
PHST- 2020/12/02 06:00 [medline]
PHST- 2020/10/07 00:00 [pmc-release]
AID - S0100-879X2020001100608 [pii]
AID - 10.1590/1414-431X202010058 [doi]
PST - epublish
SO  - Braz J Med Biol Res. 2020 Oct 7;53(11):e10058. doi: 10.1590/1414-431X202010058. 
      eCollection 2020.