PMID- 3305559
OWN - NLM
STAT- MEDLINE
DCOM- 19871021
LR  - 20211216
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 25
IP  - 8
DP  - 1987 Aug
TI  - Identification of enterotoxigenic Escherichia coli with synthetic alkaline 
      phosphatase-conjugated oligonucleotide DNA probes.
PG  - 1438-41
AB  - Alkaline phosphatase-conjugated (AP) 26-base oligonucleotide DNA probes were 
      compared with the same probes labeled with gamma-32P for the identification of 
      heat-labile (LT) and heat-stable (ST) enterotoxigenic Escherichia coli (ETEC). 
      The AP oligonucleotide probes were as sensitive as the radiolabeled (RL) probes 
      in detecting LT and STA-2 target cell DNA, but the AP ST probe, which differed 
      from STA-1 by two bases, was less sensitive than the RL probe in detecting STA-1 
      DNA (6.25 versus 0.78 ng). Of 94 ETEC that were identified with the RL probe, the 
      AP probes detected 93% (28 of 30) of ST, 73% (25 of 34) of LT, and 67% (20 of 30) 
      of LTST ETEC. When colony lysates of these ETEC were examined, the AP probes 
      identified all 94 ETEC. In examinations of stool blots, the RL and AP probes were 
      shown to have sensitivities of 71 and 59%, specificities of 91 and 86%, positive 
      predictive values of 87 and 73%, and negative predictive values of 86 and 74%, 
      respectively. AP oligonucleotide probes to detect ETEC were less sensitive in 
      detecting ETEC by colony or stool blot hybridization than the RL probes but could 
      be used by laboratories without access to radioisotopes to examine colony 
      lysates.
FAU - Seriwatana, J
AU  - Seriwatana J
FAU - Echeverria, P
AU  - Echeverria P
FAU - Taylor, D N
AU  - Taylor DN
FAU - Sakuldaipeara, T
AU  - Sakuldaipeara T
FAU - Changchawalit, S
AU  - Changchawalit S
FAU - Chivoratanond, O
AU  - Chivoratanond O
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Bacterial Toxins)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Enterotoxins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Phosphorus Radioisotopes)
RN  - 0 (heat stable toxin (E coli))
RN  - D9K3SN2LNY (heat-labile enterotoxin, E coli)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase
MH  - Bacterial Toxins/biosynthesis
MH  - Child
MH  - DNA, Bacterial/*analysis
MH  - Diarrhea/diagnosis/microbiology
MH  - Enterotoxins/biosynthesis
MH  - Escherichia coli/genetics/*isolation & purification/metabolism
MH  - Escherichia coli Infections/*diagnosis/microbiology
MH  - *Escherichia coli Proteins
MH  - Feces/microbiology
MH  - Humans
MH  - Nucleic Acid Hybridization
MH  - *Oligodeoxyribonucleotides
MH  - Phosphorus Radioisotopes
MH  - Predictive Value of Tests
PMC - PMC269242
EDAT- 1987/08/01 00:00
MHDA- 1987/08/01 00:01
PMCR- 1987/08/01
CRDT- 1987/08/01 00:00
PHST- 1987/08/01 00:00 [pubmed]
PHST- 1987/08/01 00:01 [medline]
PHST- 1987/08/01 00:00 [entrez]
PHST- 1987/08/01 00:00 [pmc-release]
AID - 10.1128/jcm.25.8.1438-1441.1987 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1987 Aug;25(8):1438-41. doi: 10.1128/jcm.25.8.1438-1441.1987.