PMID- 33057705
OWN - NLM
STAT- MEDLINE
DCOM- 20210531
LR  - 20210531
IS  - 1569-9285 (Electronic)
IS  - 1569-9285 (Linking)
VI  - 31
IP  - 5
DP  - 2020 Nov 1
TI  - Sympathetic innervation of canine pulmonary artery and morphometric and 
      functional analysis in dehydromonocrotaline-induced models after pulmonary artery 
      denervation.
PG  - 708-717
LID - 10.1093/icvts/ivaa166 [doi]
AB  - OBJECTIVES: We aimed to describe the anatomic distribution of periarterial 
      pulmonary sympathetic nerves and to observe the long-term morphometric and 
      functional changes after pulmonary artery denervation (PADN), a novel therapy for 
      pulmonary arterial hypertension (PAH). METHODS: A total of 45 beagles were 
      divided into a sympathetic innervation group (n = 3, 33.3% were females), a PAH 
      group (n = 35, 34.3% were females) and a control group (n = 7, 28.5% were 
      females). The PAH group was randomly divided into no-PADN (n = 7), instant-PADN 
      (n = 7), 1M-PADN (n = 7), 2M-PADN (n = 7) and 3M-PADN (n = 7) subgroups. The 
      sympathetic innervation group was sacrificed to reveal the sympathetic 
      innervation of pulmonary arteries. PAH was induced by injecting 
      dehydromonocrotaline (DHMCT) through the right atrium. The pulmonary capillary 
      wedge pressure, right ventricular systolic pressure, right ventricular mean 
      pressure, pulmonary artery systolic pressure and pulmonary artery mean pressure 
      of each group were continuously measured. The cardiac output was detected to 
      calculate the pulmonary vascular resistance. PAH and control groups were 
      subjected to immunofluorescence assay, sympathetic nerve conduction velocity 
      measurement and transmission electron microscopy. RESULTS: The no-PADN group had 
      significantly higher PVSP, PVMP, pulmonary artery systolic pressure, pulmonary 
      artery mean pressure and pulmonary vascular resistance but lower cardiac output 
      than those of the control group (P < 0.05). Instant-PADN, 1M-PADN, 2M-PADN and 
      3M-PADN groups had significantly lower PVSP, PVMP, pulmonary artery systolic 
      pressure, pulmonary artery mean pressure and pulmonary vascular resistance but 
      higher cardiac output than those of the no-PADN group (P < 0.05). Most 
      sympathetic nerves were located within 2.5 mm of the intimae of the bifurcation 
      and proximal trunk, mainly in the left trunk. The diameter and cross-sectional 
      area of myelinated fibres in the PAH group were significantly larger than those 
      of the control group. Sympathetic nerve conduction velocity of the PAH group 
      gradually decreased, and nerve fibres were almost demyelinated 3 months after 
      PADN. CONCLUSIONS: PADN effectively relieved dehydromonocrotaline-induced canine 
      PAH and decreased sympathetic nerve conduction velocity.
CI  - (c) The Author(s) 2020. Published by Oxford University Press on behalf of the 
      European Association for Cardio-Thoracic Surgery. All rights reserved.
FAU - Jiang, Xiaomin
AU  - Jiang X
AD  - Department of Cardiovascular Research, Nanjing Medical University, Nanjing, 
      China.
AD  - Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, 
      Nanjing, China.
FAU - Zhang, Juan
AU  - Zhang J
AD  - Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, 
      Nanjing, China.
FAU - Zhou, Ling
AU  - Zhou L
AD  - Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, 
      Nanjing, China.
FAU - Luo, Jie
AU  - Luo J
AD  - Department of Cardiovascular Research, Nanjing Medical University, Nanjing, 
      China.
FAU - Wang, Jinsong
AU  - Wang J
AD  - Department of Pathology, Nanjing First Hospital, Nanjing Medical University, 
      Nanjing, China.
FAU - Li, Li
AU  - Li L
AD  - Department of Pathology, Nanjing First Hospital, Nanjing Medical University, 
      Nanjing, China.
FAU - Chen, Shaoliang
AU  - Chen S
AD  - Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, 
      Nanjing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Interact Cardiovasc Thorac Surg
JT  - Interactive cardiovascular and thoracic surgery
JID - 101158399
RN  - 23291-96-5 (monocrotaline pyrrole)
RN  - 73077K8HYV (Monocrotaline)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Dogs
MH  - Hypertension, Pulmonary/chemically induced/*physiopathology/therapy
MH  - Lung/blood supply/physiopathology
MH  - Monocrotaline/analogs & derivatives/toxicity
MH  - Pulmonary Artery/*innervation/physiopathology
MH  - Sympathectomy/*methods
MH  - Sympathetic Nervous System/diagnostic imaging/*physiopathology
MH  - Vascular Resistance/*physiology
OTO - NOTNLM
OT  - Dehydromonocrotaline
OT  - Denervation
OT  - Pulmonary artery
OT  - Sympathetic innervation
EDAT- 2020/10/16 06:00
MHDA- 2021/06/01 06:00
CRDT- 2020/10/15 17:36
PHST- 2020/02/04 00:00 [received]
PHST- 2020/07/06 00:00 [revised]
PHST- 2020/07/20 00:00 [accepted]
PHST- 2020/10/16 06:00 [pubmed]
PHST- 2021/06/01 06:00 [medline]
PHST- 2020/10/15 17:36 [entrez]
AID - 5924433 [pii]
AID - 10.1093/icvts/ivaa166 [doi]
PST - ppublish
SO  - Interact Cardiovasc Thorac Surg. 2020 Nov 1;31(5):708-717. doi: 
      10.1093/icvts/ivaa166.