PMID- 33058414
OWN - NLM
STAT- MEDLINE
DCOM- 20211130
LR  - 20211130
IS  - 2211-5463 (Electronic)
IS  - 2211-5463 (Linking)
VI  - 10
IP  - 12
DP  - 2020 Dec
TI  - Platelet lysate induces chondrogenic differentiation of umbilical cord-derived 
      mesenchymal stem cells by regulating the lncRNA H19/miR-29b-3p/SOX9 axis.
PG  - 2656-2665
LID - 10.1002/2211-5463.13002 [doi]
AB  - Platelet lysate (PL) has been shown to induce chondrogenic differentiation of 
      human umbilical cord-derived mesenchymal stem cells (hUCMSCs). However, the 
      underlying mechanism is still not clear. The aim of this study was to investigate 
      whether long noncoding RNA H19 is involved in this process. hUCMSCs were 
      isolated, identified and cultured in 5% PL-supplemented chondrogenic 
      differentiation medium. Chondrogenic differentiation was assessed by Alcian blue 
      staining. The expressions of H19, miR-29b-3p, SRY-related high-mobility-group box 
      9 (SOX9), collagen II and aggrecan were determined by quantitative real-time PCR 
      and western blot. The interaction between miR-29b-3p and H19 or SOX9 was analyzed 
      by luciferase reporter assay. During PL-induced chondrogenic differentiation of 
      hUCMSCs, expressions of H19 and SOX9 were increased, whereas miR-29b-3p 
      expression was decreased. H19 overexpression promoted, whereas H19 silencing 
      attenuated the PL-induced chondrogenic differentiation of hUCMSCs. SOX9 was 
      identified as a direct target of miR-29b-3p, and H19 was observed to act as a 
      sponge of miR-29b-3p to up-regulate SOX9 expression. The chondrogenic 
      differentiation-promoting effect of H19 overexpression was negated when 
      miR-29b-3p expression was up-regulated by Lenti-miR-29b-3p infection. In 
      conclusion, PL induced chondrogenic differentiation of hUCMSCs by regulating the 
      H19/miR-29b-3p/SOX9 axis.
CI  - (c) 2020 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.
FAU - Cao, Boran
AU  - Cao B
AUID- ORCID: 0000-0003-2633-0772
AD  - Department of Orthopedics, The First Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
FAU - Dai, Xin
AU  - Dai X
AD  - Department of Oncology, The First Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201106
PL  - England
TA  - FEBS Open Bio
JT  - FEBS open bio
JID - 101580716
RN  - 0 (H19 long non-coding RNA)
RN  - 0 (MIRN29B1 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (SOX9 Transcription Factor)
RN  - 0 (SOX9 protein, human)
SB  - IM
MH  - Blood Platelets/metabolism
MH  - Cell Differentiation
MH  - Cell Line
MH  - Chondrocytes/*metabolism
MH  - Humans
MH  - Mesenchymal Stem Cells/*metabolism
MH  - MicroRNAs/genetics/metabolism
MH  - RNA, Long Noncoding/genetics/*metabolism
MH  - SOX9 Transcription Factor/genetics/*metabolism
MH  - Umbilical Cord/cytology/*metabolism
PMC - PMC7714074
OTO - NOTNLM
OT  - H19
OT  - SOX9
OT  - chondrogenic differentiation
OT  - mesenchymal stem cells
OT  - miR-29b-3p
OT  - platelet lysate
COIS- The authors declare no conflict of interest.
EDAT- 2020/10/16 06:00
MHDA- 2021/12/01 06:00
PMCR- 2020/11/06
CRDT- 2020/10/15 17:45
PHST- 2020/08/04 00:00 [received]
PHST- 2020/08/20 00:00 [revised]
PHST- 2020/10/10 00:00 [accepted]
PHST- 2020/10/16 06:00 [pubmed]
PHST- 2021/12/01 06:00 [medline]
PHST- 2020/10/15 17:45 [entrez]
PHST- 2020/11/06 00:00 [pmc-release]
AID - FEB413002 [pii]
AID - 10.1002/2211-5463.13002 [doi]
PST - ppublish
SO  - FEBS Open Bio. 2020 Dec;10(12):2656-2665. doi: 10.1002/2211-5463.13002. Epub 2020 
      Nov 6.