PMID- 33062923
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220417
IS  - 2476-4108 (Print)
IS  - 2476-3772 (Electronic)
IS  - 2476-3772 (Linking)
VI  - 18
IP  - 9
DP  - 2020 Sep
TI  - Zinc attenuates ecstasy-induced apoptosis through downregulation of caspase-3 in 
      cultured TM3 cells: An experimental study.
PG  - 777-784
LID - 10.18502/ijrm.v13i9.7672 [doi]
AB  - BACKGROUND: 3, 4-Methylenedioxymethamphetamine (MDMA) is commonly known as the 
      most famous amphetamine derivative. OBJECTIVE: To evaluate the influence of zinc 
      on MDMA-induced apoptosis and caspase- 3 gene expression in Leydig cell line 
      (TM3). MATERIALS AND METHODS: Leydig cells were studied in differenet treatment 
      groups regarding MDMA (0, 0.5, 1, 3, 5 mM) and zinc (0, 4, 8, 16, 32 muM). By the 
      way, the effective concentration was determined to be 5 mM for MDMA and 8 muM for 
      zinc. Then, TM3 cells were cultured in free medium as control (group I), medium 
      containing MDMA (5 mM) (group II), zinc (8 microM) (group III), and zinc (8 microM) prior 
      to MDMA (5 mM) (group IV) as well as in an untreated group (control). Cell 
      viability was assessed at different times after cell culture by MTT assay. The 
      mRNA expression level of caspase-3 was analyzed using real-time quantitative 
      polymerase chain reaction. RESULTS: The cellular viability was significantly 
      reduced in TM3 cells after 24 hr and 48 hr exposure time regarding different 
      concentrations of MDMA as well as high concentration of zinc (16 and 32 muM). Cell 
      viability was increased in the group that received zinc (8 microM) before addition of 
      MDMA (5 mM) compared to the control and MDMA groups. The mean +/- SE of fold was 
      22.40 +/- 7.5, 0.06 +/- 0.02, and 0.009 +/- 0.003 in MDMA, zinc, and zinc + MDMA 
      groups, respectively. The mean of caspase-3 mRNA level was significantly 
      increased in the MDMA-treated group (5 mM), while the relative expression of 
      caspase-3 gene was significantly decreased in the zinc (8 microM) + MDMA (5 mM) group 
      compared with the MDMA (5 mM) group (p = 0.001). CONCLUSION: Dietary intake of 
      zinc has a protective effect against MDMA consumption in mouse.
CI  - Copyright (c) 2020 Lozeie et al.
FAU - Lozeie, Marziyeh
AU  - Lozeie M
AD  - Islamic Azad University, Tabriz Complex, Tabriz, Iran.
FAU - Bagheri, Morteza
AU  - Bagheri M
AD  - Cellular and Molecular Research Center, Cellular and Molecular Medicine 
      Institute, Urmia University of Medical Sciences, Urmia, Iran.
FAU - Rad, Isa Abdi
AU  - Rad IA
AD  - Cellular and Molecular Research Center, Cellular and Molecular Medicine 
      Institute, Urmia University of Medical Sciences, Urmia, Iran.
FAU - Hossein-Zadeh, Nadia
AU  - Hossein-Zadeh N
AD  - Islamic Azad University, Tabriz Complex, Tabriz, Iran.
FAU - Nasir-Zadeh, Mahdyieh
AU  - Nasir-Zadeh M
AD  - Cellular and Molecular Research Center, Cellular and Molecular Medicine 
      Institute, Urmia University of Medical Sciences, Urmia, Iran.
LA  - eng
PT  - Journal Article
DEP - 20200920
PL  - Iran
TA  - Int J Reprod Biomed
JT  - International journal of reproductive biomedicine
JID - 101679102
PMC - PMC7521166
OTO - NOTNLM
OT  - Apoptosis
OT  - MDMA
OT  - TM3 cells
OT  - Zinc
COIS- None.
EDAT- 2020/10/17 06:00
MHDA- 2020/10/17 06:01
PMCR- 2020/09/20
CRDT- 2020/10/16 05:57
PHST- 2019/05/26 00:00 [received]
PHST- 2019/12/29 00:00 [revised]
PHST- 2020/04/21 00:00 [accepted]
PHST- 2020/10/16 05:57 [entrez]
PHST- 2020/10/17 06:00 [pubmed]
PHST- 2020/10/17 06:01 [medline]
PHST- 2020/09/20 00:00 [pmc-release]
AID - 10.18502/ijrm.v13i9.7672 [doi]
PST - epublish
SO  - Int J Reprod Biomed. 2020 Sep 20;18(9):777-784. doi: 10.18502/ijrm.v13i9.7672. 
      eCollection 2020 Sep.