PMID- 33064361
OWN - NLM
STAT- MEDLINE
DCOM- 20210624
LR  - 20210624
IS  - 2162-3279 (Electronic)
VI  - 10
IP  - 12
DP  - 2020 Dec
TI  - Role of densin-180 in mouse ventral hippocampal neurons in 24-hr retention of 
      contextual fear conditioning.
PG  - e01891
LID - 10.1002/brb3.1891 [doi]
LID - e01891
AB  - INTRODUCTION: Densin-180 interacts with postsynaptic molecules including 
      calcium/calmodulin-dependent protein kinase IIalpha (CaMKIIalpha) but its function in 
      learning and memory process has been unclear. METHODS: To investigate a role of 
      hippocampal densin-180 in contextual fear conditioning (CFC) learning and memory 
      processes, knockdown (KD) of densin-180 in hippocampal subareas was applied. 
      RESULTS: First, ventral hippocampal (vHC) densin-180 KD impaired single-trial CFC 
      (stCFC) memory one day later. stCFC caused freezing behaviors to reach the peak 
      about one hour later in both control and KD mice, but then freezing was 
      disappeared at 2 hr postshock in KD mice. Second, stCFC caused an immediate and 
      transient reduction of vHC densin-180 in control mice, which was not observed in 
      KD mice. Third, stCFC caused phosphorylated-T286 (p-T286) CaMKIIalpha to change 
      similarly to densin-180, but p-T305 CaMKIIalpha was increased 1 hr later in control 
      mice. In KD mice, these effects were gone. Moreover, both basal levels of p-T286 
      and p-T305 CaMKIIalpha were reduced without change in total CaMKIIalpha in KD mice. 
      Fourth, we found double-trial CFC (dtCFC) memory acquisition and retrieval 
      kinetics were different from those of stCFC in vHC KD mice. In addition, 
      densin-180 in dorsal hippocampal area appeared to play its unique role during the 
      very early retrieval period of both CFC memories. CONCLUSION: This study shows 
      that vHC densin-180 is necessary for stCFC memory formation and retrieval and 
      suggests that both densin-180 and p-T305 CaMKIIalpha at 1 ~ 2 hr postshock are 
      important for stCFC memory formation. We conclude that roles of hippocampal 
      neuronal densin-180 in CFC are temporally dynamic and differential depending on 
      the pattern of conditioning stimuli and its location along the dorsoventral axis 
      of hippocampal formation.
CI  - (c) 2020 The Authors. Brain and Behavior published by Wiley Periodicals LLC.
FAU - Kim, Chong-Hyun
AU  - Kim CH
AUID- ORCID: 0000-0002-0968-4176
AD  - Center for Neuroscience, Brain Science Institute, Korea Institute of Science and 
      Technology, Seoul, Korea.
AD  - Neuroscience Program, Division of Bio-Medical Science and Technology, KIST 
      School, Korea University of Science and Technology (UST), Seoul, Korea.
FAU - Kim, Seoyul
AU  - Kim S
AD  - Center for Neuroscience, Brain Science Institute, Korea Institute of Science and 
      Technology, Seoul, Korea.
AD  - Neuroscience Program, Division of Bio-Medical Science and Technology, KIST 
      School, Korea University of Science and Technology (UST), Seoul, Korea.
FAU - Kim, Su-Hyun
AU  - Kim SH
AUID- ORCID: 0000-0001-5006-3286
AD  - Center for Neuroscience, Brain Science Institute, Korea Institute of Science and 
      Technology, Seoul, Korea.
AD  - Neuroscience Program, Division of Bio-Medical Science and Technology, KIST 
      School, Korea University of Science and Technology (UST), Seoul, Korea.
FAU - Roh, Jongtae
AU  - Roh J
AUID- ORCID: 0000-0003-1114-8580
AD  - Center for Neuroscience, Brain Science Institute, Korea Institute of Science and 
      Technology, Seoul, Korea.
AD  - Neuroscience Program, Division of Bio-Medical Science and Technology, KIST 
      School, Korea University of Science and Technology (UST), Seoul, Korea.
FAU - Jin, Harin
AU  - Jin H
AD  - Center for Neuroscience, Brain Science Institute, Korea Institute of Science and 
      Technology, Seoul, Korea.
AD  - Neuroscience Program, Division of Bio-Medical Science and Technology, KIST 
      School, Korea University of Science and Technology (UST), Seoul, Korea.
FAU - Song, Bokyung
AU  - Song B
AUID- ORCID: 0000-0002-3803-0235
AD  - Center for Neuroscience, Brain Science Institute, Korea Institute of Science and 
      Technology, Seoul, Korea.
AD  - Neuroscience Program, Division of Bio-Medical Science and Technology, KIST 
      School, Korea University of Science and Technology (UST), Seoul, Korea.
LA  - eng
GR  - 2016M3C7A1905119/National Research Foundation of Korea/
GR  - 2E30190 and 2E30762/Korea Institute of Science and Technology/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201016
PL  - United States
TA  - Brain Behav
JT  - Brain and behavior
JID - 101570837
SB  - IM
MH  - Animals
MH  - Conditioning, Classical
MH  - *Fear
MH  - *Hippocampus
MH  - Memory
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neurons
PMC - PMC7749528
OTO - NOTNLM
OT  - CaMKIIalpha
OT  - contextual fear conditioning
OT  - densin-180
OT  - hippocampus
OT  - memory
OT  - retrieval
COIS- The authors have declared that there exists no conflict of interest to declare.
EDAT- 2020/10/17 06:00
MHDA- 2021/06/25 06:00
PMCR- 2020/10/16
CRDT- 2020/10/16 12:12
PHST- 2020/04/27 00:00 [received]
PHST- 2020/09/01 00:00 [revised]
PHST- 2020/09/23 00:00 [accepted]
PHST- 2020/10/17 06:00 [pubmed]
PHST- 2021/06/25 06:00 [medline]
PHST- 2020/10/16 12:12 [entrez]
PHST- 2020/10/16 00:00 [pmc-release]
AID - BRB31891 [pii]
AID - 10.1002/brb3.1891 [doi]
PST - ppublish
SO  - Brain Behav. 2020 Dec;10(12):e01891. doi: 10.1002/brb3.1891. Epub 2020 Oct 16.