PMID- 33066719
OWN - NLM
STAT- MEDLINE
DCOM- 20211227
LR  - 20220902
IS  - 2162-1918 (Print)
IS  - 2162-1934 (Electronic)
IS  - 2162-1918 (Linking)
VI  - 10
IP  - 9
DP  - 2021 Sep
TI  - High-Voltage, Pulsed Electric Fields Eliminate Pseudomonas aeruginosa Stable 
      Infection in a Mouse Burn Model.
PG  - 477-489
LID - 10.1089/wound.2019.1147 [doi]
AB  - Objective: The incidence of severe infectious complications after burn injury 
      increases mortality by 40%. However, traditional approaches for managing burn 
      infections are not always effective. High-voltage, pulsed electric field (PEF) 
      treatment shortly after a burn injury has demonstrated an antimicrobial effect in 
      vivo; however, the working parameters and long-term effects of PEF treatment have 
      not yet been investigated. Approach: Nine sets of PEF parameters were 
      investigated to optimize the applied voltage, pulse duration, and frequency or 
      pulse repetition for disinfection of Pseudomonas aeruginosa infection in a stable 
      mouse burn wound model. The bacterial load after PEF administration was monitored 
      for 3 days through bioluminescence imaging. Histological assessments and 
      inflammation response analyses were performed at 1 and 24 h after the therapy. 
      Results: Among all tested PEF parameters, the best disinfection efficacy of P. 
      aeruginosa infection was achieved with a combination of 500 V, 100 mus, and 200 
      pulses delivered at 3 Hz through two plate electrodes positioned 1 mm apart for 
      up to 3 days after the injury. Histological examinations revealed fewer 
      inflammatory signs in PEF-treated wounds compared with untreated infected burns. 
      Moreover, the expression levels of multiple inflammatory-related cytokines 
      (interleukin [IL]-1alpha/beta, IL-6, IL-10, leukemia inhibitory factor [LIF], and tumor 
      necrosis factor-alpha [TNF-alpha]), chemokines (macrophage inflammatory protein 
      [MIP]-1alpha/beta and monocyte chemoattractant protein-1 [MCP-1]), and 
      inflammation-related factors (vascular endothelial growth factor [VEGF], 
      macrophage colony-stimulating factor [M-CSF], and granulocyte-macrophage 
      colony-stimulating factor [G-CSF]) were significantly decreased in the infected 
      burn wound after PEF treatment. Innovation: We showed that PEF treatment on 
      infected wounds reduces the P. aeruginosa load and modulates inflammatory 
      responses. Conclusion: The data presented in this study suggest that PEF 
      treatment is a potent candidate for antimicrobial therapy for P. aeruginosa burn 
      infections.
FAU - Wu, Mengjie
AU  - Wu M
AD  - Department of Orthodontics, The Affiliated Stomatology Hospital, Zhejiang 
      University School of Medicine, Hangzhou, China.
AD  - Center of Engineering in Medicine, Massachusetts General Hospital, Harvard 
      Medical School, Boston, Massachusetts, USA.
FAU - Rubin, Andrey Ethan
AU  - Rubin AE
AD  - Porter School of Environment and Earth Sciences, Tel Aviv University, Tel Aviv, 
      Israel.
FAU - Dai, Tianhong
AU  - Dai T
AD  - Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical 
      School, Boston, Massachusetts, USA.
AD  - Vaccine and Immunotherapy Center, Massachusetts General Hospital, Harvard Medical 
      School, Boston, Massachusetts, USA.
FAU - Schloss, Rene
AU  - Schloss R
AD  - Department of Biomedical Engineering, Rutgers University, Piscataway, New Jersey, 
      USA.
FAU - Usta, Osman Berk
AU  - Usta OB
AD  - Center of Engineering in Medicine, Massachusetts General Hospital, Harvard 
      Medical School, Boston, Massachusetts, USA.
FAU - Golberg, Alexander
AU  - Golberg A
AD  - Porter School of Environment and Earth Sciences, Tel Aviv University, Tel Aviv, 
      Israel.
FAU - Yarmush, Martin
AU  - Yarmush M
AD  - Center of Engineering in Medicine, Massachusetts General Hospital, Harvard 
      Medical School, Boston, Massachusetts, USA.
AD  - Department of Biomedical Engineering, Rutgers University, Piscataway, New Jersey, 
      USA.
AD  - Shriners Burn Hospital for Children, Boston, Massachusetts, USA.
LA  - eng
GR  - R21 AI142415/AI/NIAID NIH HHS/United States
GR  - R21 AI163950/AI/NIAID NIH HHS/United States
GR  - R21 GM136002/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20201218
PL  - United States
TA  - Adv Wound Care (New Rochelle)
JT  - Advances in wound care
JID - 101590593
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Animals
MH  - Burns/complications/microbiology/*therapy
MH  - Disease Models, Animal
MH  - Disinfection/*methods
MH  - Electric Stimulation Therapy/*methods
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - Inflammation
MH  - Pseudomonas Infections/*therapy
MH  - Pseudomonas aeruginosa
MH  - Sepsis/etiology/immunology
MH  - Tachycardia
MH  - Vascular Endothelial Growth Factor A
MH  - Wound Infection/microbiology/*therapy
PMC - PMC8260897
OTO - NOTNLM
OT  - burn infection
OT  - disinfection
OT  - inflammation
OT  - pulsed electric field
COIS- No competing financial interests exist. The authors listed expressly wrote the 
      content of this article. No ghostwriters were used to write this article.
EDAT- 2020/10/18 06:00
MHDA- 2021/12/28 06:00
PMCR- 2022/09/01
CRDT- 2020/10/17 05:18
PHST- 2020/10/18 06:00 [pubmed]
PHST- 2021/12/28 06:00 [medline]
PHST- 2020/10/17 05:18 [entrez]
PHST- 2022/09/01 00:00 [pmc-release]
AID - 10.1089/wound.2019.1147 [pii]
AID - 10.1089/wound.2019.1147 [doi]
PST - ppublish
SO  - Adv Wound Care (New Rochelle). 2021 Sep;10(9):477-489. doi: 
      10.1089/wound.2019.1147. Epub 2020 Dec 18.