PMID- 33067940
OWN - NLM
STAT- MEDLINE
DCOM- 20201020
LR  - 20201020
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 28
IP  - 5
DP  - 2020 Oct
TI  - [Fluorescence in Situ Hybridization for Detecting Molecular Cytogenetic 
      Abnormalities of Chronic Lymphocytic Leukemia].
PG  - 1474-1479
LID - 10.19746/j.cnki.issn.1009-2137.2020.05.008 [doi]
AB  - OBJECTIVE: To investigate the value of fluorescence in situ hybridization (FISH) 
      in the diagnosis and prognosis evaluation of patients with chronic lymphocytic 
      leukemia (CLL). METHODS: Ninty-three patients with newly diagnosed CLL were 
      tested by five probes including RB1 (13q14.1), D13S25 (13q14.3), p53(17p13.1), 
      ATM( 11q22.3) and CSP12, while conventional cytogenetics (CC) was used for 
      karyotype analysis. Then the correlation of the molecular cytogenetic 
      abnormalities with the clinical Binet stages, Rai stages and the other related 
      laboratory examinations was analyzed. RESULTS: The detection rate of chromosome 
      abnormality in 93 patients was 79.6%, out of which detection rate of 13q (13q- 
      was the highest and accounted for 45.2%), followed by trisomy 12 (+12) 26.9%, p53 
      deletion (17p-) 19.4% and ATM deletion (11p-) 17.2%. There were 27 cases (29.0%) 
      with 2 or more abnormalities, including 13 cases with 13q-/17q-, 5 with 
      13q-/11q-, and 4 with 13q-/+12. Compared with CC test results, the positive rate 
      of FISH detection was significantly higher (chi(2)=32.127, P＜0.01). There was no 
      significant correlation between FISH results and Rai stages (P＞0.05), meanwhile 
      17p- highly correlated with later stage of the Binet stages (P=0.012). The 
      molecular cytogenetic abnormalities significantly correlated with age, absolute 
      value of peripheral lymphocyte count and CD38 expression level (P＞0.05). The 
      incidence of 13q- in female (65.4%) was statistically significantly higher than 
      that in male (37.3%) (P=0.015). The unmutated IGHV rate of CLL patients with a 
      17p- was significantly higher than that in patients without this genetic 
      abnormality (P=0.013). The expression of CD38 was detected among 29.0% of the 
      patients, which significantly correlated with Binet stages (P=0.027) and 
      unmutated IGHV (P=0.006). CONCLUSION: FISH can greatly increase the detection 
      rate of molecular cytogenetic abnormalities in CLL patients, which, as a powerful 
      supplement to the conventional cytogenetics, can be applied for the clinical 
      staging and prognosis evaluation of CLL patients.
FAU - Zhang, Hai-Ying
AU  - Zhang HY
AD  - Department of Pathology, The 940th Hospital of Joint Logistic Support of Chinese 
      PLA,Lanzhou 730050, Gansu Province, China.
FAU - Yang, Yan-Li
AU  - Yang YL
AD  - Department of Pathology, The 940th Hospital of Joint Logistic Support of Chinese 
      PLA,Lanzhou 730050, Gansu Province, China.
FAU - Zhao, Qiang
AU  - Zhao Q
AD  - Department of Hematology, The 940th Hospital of Joint Logistic Support of Chinese 
      PLA, Lanzhou 730050, Gansu Province, China.
FAU - Zhang, Jing
AU  - Zhang J
AD  - Department of Hematology, The 940th Hospital of Joint Logistic Support of Chinese 
      PLA, Lanzhou 730050, Gansu Province, China.
FAU - Bai, Hai
AU  - Bai H
AD  - Department of Hematology, The 940th Hospital of Joint Logistic Support of Chinese 
      PLA, Lanzhou 730050, Gansu Province, China,E-mail: baihai98@tom.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
SB  - IM
MH  - Chromosome Aberrations
MH  - Cytogenetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - *Leukemia, Lymphocytic, Chronic, B-Cell/diagnosis/genetics
MH  - Male
EDAT- 2020/10/18 06:00
MHDA- 2020/10/21 06:00
CRDT- 2020/10/17 08:33
PHST- 2020/10/17 08:33 [entrez]
PHST- 2020/10/18 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
AID - 1009-2137(2020)05-1474-06 [pii]
AID - 10.19746/j.cnki.issn.1009-2137.2020.05.008 [doi]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2020 Oct;28(5):1474-1479. doi: 
      10.19746/j.cnki.issn.1009-2137.2020.05.008.