PMID- 33069947
OWN - NLM
STAT- MEDLINE
DCOM- 20210108
LR  - 20210108
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 208
DP  - 2021 Jan 15
TI  - Inhibition in growth and cardiotoxicity of tris (2-butoxyethyl) phosphate through 
      down-regulating Wnt signaling pathway in early developmental stage of zebrafish 
      (Danio rerio).
PG  - 111431
LID - S0147-6513(20)31268-9 [pii]
LID - 10.1016/j.ecoenv.2020.111431 [doi]
AB  - As a common organophosphorus flame retardant, tris (2-butoxyethyl) phosphate 
      (TBOEP) is detected in water environment and aquatic animals extensively. Despite 
      previous researches have reported the developmental toxicity of TBOEP in 
      zebrafish (Danio rerio) larvae, few research focused on its underlying 
      mechanisms. In this study, zebrafish embryos were exposed to 0, 20, 200, 1000 and 
      2000 microg/L TBOEP from 2 until 120 h post-fertilization (hpf) to determine 
      potential mechanisms of developmental toxicity of this compound. Early 
      developmental stage parameters such as body length, survival rate, hatching rate 
      and heart rate were decreased, while malformation rate was ascended. Quantitative 
      reverse transcription polymerase chain reaction (qRT-PCR) assay was carried out 
      at 12, 24, 72 and 120 hpf to demonstrate alterations in expression of genes of 
      Wnt signaling pathway. The results indicated that axin1 was significantly 
      up-regulated, while beta-catenin, pkc and wnt11 were down-regulated. Correlation 
      analysis indicated that expression of these genes was significantly correlated 
      with body length. Furthermore, apoptosis was detected in heart region by acridine 
      orange (AO) staining and terminal deoxynucleotide transferase-mediated deoxy-UTP 
      nick end labeling (TUNEL) assay. In addition, at 120 hpf, occurrence of oxidative 
      stress was observed in zebrafish larvae. Moreover, 6-Bromoindirubin-3'-oxime 
      (BIO)， an activator of Wnt pathway, was found to alleviate the inhibiting effects 
      of TBOEP on zebrafish growth. The overall outcomes offered novel viewpoints in 
      toxic effects of TBOEP, and down-regulating Wnt signaling pathway were able to 
      reveal some potential mechanisms of developmental toxicity of TBOEP in zebrafish 
      larvae.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Xiong, Hao
AU  - Xiong H
AD  - College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
FAU - Huang, Yangyang
AU  - Huang Y
AD  - College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
FAU - Mao, Yuchao
AU  - Mao Y
AD  - College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
FAU - Liu, Chunsheng
AU  - Liu C
AD  - College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China; Key 
      Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural 
      University, Wuhan 430070, China.
FAU - Wang, Jianghua
AU  - Wang J
AD  - College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China; Key 
      Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural 
      University, Wuhan 430070, China. Electronic address: whtjwjh@163.com.
LA  - eng
PT  - Journal Article
DEP - 20201016
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (Flame Retardants)
RN  - 0 (Organophosphates)
RN  - 0 (Organophosphorus Compounds)
RN  - 0 (Phosphates)
RN  - 0 (Plasticizers)
RN  - 0 (Water Pollutants, Chemical)
RN  - RYA6940G86 (tris(2-butoxyethyl) phosphate)
SB  - IM
MH  - Animals
MH  - Cardiotoxicity
MH  - Down-Regulation
MH  - Embryo, Nonmammalian/drug effects
MH  - Flame Retardants/metabolism
MH  - In Situ Nick-End Labeling
MH  - Larva/drug effects
MH  - Organophosphates/metabolism
MH  - Organophosphorus Compounds/metabolism/*toxicity
MH  - Phosphates/metabolism
MH  - Plasticizers/*toxicity
MH  - Water Pollutants, Chemical/metabolism/*toxicity
MH  - Wnt Signaling Pathway/physiology
MH  - Zebrafish/metabolism/*physiology
OTO - NOTNLM
OT  - Cardiotoxicity
OT  - Developmental toxicity
OT  - Oxidative stress
OT  - Tris (2-butoxyethyl) phosphate
OT  - Wnt signaling pathway
OT  - Zebrafish larvae
EDAT- 2020/10/19 06:00
MHDA- 2021/01/09 06:00
CRDT- 2020/10/18 20:27
PHST- 2020/05/19 00:00 [received]
PHST- 2020/09/21 00:00 [revised]
PHST- 2020/09/27 00:00 [accepted]
PHST- 2020/10/19 06:00 [pubmed]
PHST- 2021/01/09 06:00 [medline]
PHST- 2020/10/18 20:27 [entrez]
AID - S0147-6513(20)31268-9 [pii]
AID - 10.1016/j.ecoenv.2020.111431 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2021 Jan 15;208:111431. doi: 
      10.1016/j.ecoenv.2020.111431. Epub 2020 Oct 16.