PMID- 33070828
OWN - NLM
STAT- MEDLINE
DCOM- 20201020
LR  - 20201020
IS  - 1000-8020 (Print)
IS  - 1000-8020 (Linking)
VI  - 49
IP  - 5
DP  - 2020 Sep
TI  - [Determination of sennoside B, sennoside A and physcion in slimming health 
      foods].
PG  - 809-814
LID - 10.19813/j.cnki.weishengyanjiu.2020.05.020 [doi]
AB  - OBJECTIVE: To establish a quantitative analysis method for sennoside A, sennoside 
      B and physcion by ultra-high performance liquid chromatography-tandem mass 
      spectrometry(UPLC-MS/MS). METHODS: The sample was extracted by methanol-2 mmol/L 
      ammonium formate(9ratio1) at 40 ℃ for 1 h. The separation was performed using Agilent 
      Eclipse Plus C_(18 )(2. 1 mm x 50 mm, 1. 8 mum) column with gradient elution. The 
      mobile phase was consisted of 0. 1% formic acid and methanol. Qualitative and 
      quantitative analysis was conducted with an electrospray ionization source 
      operated in the negative ionization(ESI~-) mode and multiple reaction 
      monitoring(MRM) mode. RESULTS: The linear range of three compounds were from 0. 1 
      to 10 mug/mL with the correlation coefficients(r) above 0. 995. The spiked 
      recoveries were in the range of 81. 9% to 114. 5% at the concentrations of 0. 02, 
      0. 15 and 1. 60 mg/g with relative standard devisions(RSDs) ranged from 0. 30% to 
      3. 43%(n=6). The detection limits of sennoside A and sennoside B were 1. 2 mug/g. 
      The detection limit of physcion was 2. 4 mug/g. Sennoside A, sennoside B or 
      physcion were detected in 19 out of 40 batches of samples. The content of 
      sennoside A ranged from 0. 184 to 6. 33 mg/g and the content of sennoside B 
      ranged from 0. 202 to 7. 23 mg/g. The content of physcion ranged from 0. 042 to 
      0. 79 mg/g. CONCLUSION: The method is simple, accurate and suitable for the 
      determination of sennoside A, sennoside B and physcion.
FAU - Diao, Feiyan
AU  - Diao F
AD  - Shandong Institute for Food and Drug Control, Shandong Research Center of 
      Engineering and Technology for Safety Inspection of Food and Drug, Ji'nan 250101, 
      China.
FAU - Xie, Qiangsheng
AU  - Xie Q
AD  - Shandong Institute for Food and Drug Control, Shandong Research Center of 
      Engineering and Technology for Safety Inspection of Food and Drug, Ji'nan 250101, 
      China.
FAU - Wu, Xiaoyun
AU  - Wu X
AD  - Shandong Institute for Food and Drug Control, Shandong Research Center of 
      Engineering and Technology for Safety Inspection of Food and Drug, Ji'nan 250101, 
      China.
FAU - Liu, Chunlin
AU  - Liu C
AD  - Shandong Institute for Food and Drug Control, Shandong Research Center of 
      Engineering and Technology for Safety Inspection of Food and Drug, Ji'nan 250101, 
      China.
FAU - Li, Xiuhui
AU  - Li X
AD  - Shandong Institute for Food and Drug Control, Shandong Research Center of 
      Engineering and Technology for Safety Inspection of Food and Drug, Ji'nan 250101, 
      China.
FAU - Li, Qiyan
AU  - Li Q
AD  - Shandong Institute for Food and Drug Control, Shandong Research Center of 
      Engineering and Technology for Safety Inspection of Food and Drug, Ji'nan 250101, 
      China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Wei Sheng Yan Jiu
JT  - Wei sheng yan jiu = Journal of hygiene research
JID - 9426367
RN  - 0 (Sennosides)
RN  - H6PT94IV61 (physcione)
RN  - KA46RNI6HN (Emodin)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Liquid
MH  - Emodin/analogs & derivatives
MH  - *Sennosides
MH  - *Tandem Mass Spectrometry
OTO - NOTNLM
OT  - UPLC-MS/MS
OT  - physcion
OT  - sennoside A
OT  - sennoside B
OT  - slimming health foods
EDAT- 2020/10/20 06:00
MHDA- 2020/10/21 06:00
CRDT- 2020/10/19 05:25
PHST- 2020/10/19 05:25 [entrez]
PHST- 2020/10/20 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
AID - 10.19813/j.cnki.weishengyanjiu.2020.05.020 [doi]
PST - ppublish
SO  - Wei Sheng Yan Jiu. 2020 Sep;49(5):809-814. doi: 
      10.19813/j.cnki.weishengyanjiu.2020.05.020.