PMID- 33071640
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240329
IS  - 1445-5781 (Print)
IS  - 1447-0578 (Electronic)
IS  - 1445-5781 (Linking)
VI  - 19
IP  - 4
DP  - 2020 Oct
TI  - High stretch cycling inhibits the morphological and biological decidual process 
      in human endometrial stromal cells.
PG  - 378-384
LID - 10.1002/rmb2.12341 [doi]
AB  - PURPOSE: Subendometrial myometrium exerts wave-like activity throughout the 
      menstrual cycle, and uterine peristalsis is markedly reduced during the 
      implantation phase. We hypothesized that abnormal uterine peristalsis has an 
      adverse effect on the endometrial decidualization process. We conducted an in 
      vitro culture experiment to investigate the effect of cyclic stretch on the 
      morphological and biological endometrial decidual process. METHODS: Primary human 
      endometrial stromal cells (HESCs) were isolated from hysterectomy specimens and 
      incubated with or without 8-bromo-cyclic adenosine monophosphate (8-br-cAMP) and 
      medroxyprogesterone acetate (MPA) for 3 days. After decidualization, cultures 
      were continued for 24 hours with or without cyclic stretch using a 
      computer-operated cell tension system. RESULTS: Cyclic stretch significantly 
      repressed expression of decidual markers including insulin-like growth 
      factor-binding protein 1 (IGFBP1), prolactin (PRL), forkhead box O1 (FOXO1), and 
      WNT4 on decidualized HESCs. In addition, cyclic stretch of decidualized HESCs 
      affected the decidual morphological phenotype to an elongated shape. The 
      alternation of F-actin localization in decidualized HESCs was not observed in 
      response to cyclic stretch. CONCLUSIONS: These data suggest that cyclic stretch 
      inhibits the morphological and biological decidual process of HESCs. Our findings 
      imply that uterine abnormal contractions during the implantation period impair 
      endometrial decidualization and contribute to infertility.
CI  - (c) 2020 The Authors. Reproductive Medicine and Biology published by John Wiley & 
      Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine.
FAU - Saito, Ryohei
AU  - Saito R
AD  - Department of Obstetrics and Gynecology Saitama Medical University Iruma-gun 
      Japan.
FAU - Kajihara, Takeshi
AU  - Kajihara T
AUID- ORCID: 0000-0002-4853-9297
AD  - Department of Obstetrics and Gynecology Saitama Medical University Iruma-gun 
      Japan.
FAU - Takamura, Masashi
AU  - Takamura M
AD  - Department of Obstetrics and Gynecology Saitama Medical University Iruma-gun 
      Japan.
FAU - Tochigi, Hideno
AU  - Tochigi H
AD  - Department of Obstetrics and Gynecology Saitama Medical University Iruma-gun 
      Japan.
FAU - Sato, Tsuyoshi
AU  - Sato T
AD  - Department of Oral and Maxillofacial Surgery Saitama Medical University Iruma-gun 
      Japan.
FAU - Ishihara, Osamu
AU  - Ishihara O
AD  - Department of Obstetrics and Gynecology Saitama Medical University Iruma-gun 
      Japan.
LA  - eng
PT  - Journal Article
DEP - 20200720
PL  - Japan
TA  - Reprod Med Biol
JT  - Reproductive medicine and biology
JID - 101213278
PMC - PMC7542020
OTO - NOTNLM
OT  - cyclic stretch
OT  - endometrial decidualization
OT  - forkhead box O1
OT  - insulin-like growth factor-binding protein 1
OT  - prolactin
COIS- Conflict of interest: The authors declare no conflict of interest. Human rights 
      statement and informed consent: All procedures followed were in accordance with 
      the ethical standards of the responsible committee on human experimentation 
      (institution and national) and with the Helsinki Declaration of 1964 and its 
      later amendments. Informed consent was obtained from all patients included in the 
      study. The Institutional Review Board of the Saitama Medical University Hospital 
      approved this study.
EDAT- 2020/10/20 06:00
MHDA- 2020/10/20 06:01
PMCR- 2020/07/20
CRDT- 2020/10/19 05:54
PHST- 2020/05/25 00:00 [received]
PHST- 2020/06/20 00:00 [revised]
PHST- 2020/07/02 00:00 [accepted]
PHST- 2020/10/19 05:54 [entrez]
PHST- 2020/10/20 06:00 [pubmed]
PHST- 2020/10/20 06:01 [medline]
PHST- 2020/07/20 00:00 [pmc-release]
AID - RMB212341 [pii]
AID - 10.1002/rmb2.12341 [doi]
PST - epublish
SO  - Reprod Med Biol. 2020 Jul 20;19(4):378-384. doi: 10.1002/rmb2.12341. eCollection 
      2020 Oct.