PMID- 33077739
OWN - NLM
STAT- MEDLINE
DCOM- 20210119
LR  - 20210119
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Oct 19
TI  - Pre-diagnostic biomarkers of type 2 diabetes identified in the UAE's obese 
      national population using targeted metabolomics.
PG  - 17616
LID - 10.1038/s41598-020-73384-7 [doi]
LID - 17616
AB  - Currently, type 2 diabetes mellitus (T2DM) and obesity are major global public 
      health issues, and their prevalence in the United Arab Emirates (UAE) are among 
      the highest in the world. In 2019, The UAE diabetes national prevalence was 
      15.4%. In recent years there has been a considerable investigation of predictive 
      biomarkers associated with these conditions. This study analysed fasting (8 h) 
      blood samples from an obese, normoglycemic cohort and an obese, T2DM cohort of 
      UAE nationals, employing clinical chemistry analysis, 1D (1)H NMR and mass 
      spectroscopy (FIA-MS/MS and LC-MS/MS) techniques. The novel findings reported for 
      the first time in a UAE population revealed significant differences in a number 
      of metabolites in the T2DM cohort. Metabolic fingerprints identified by NMR 
      included BCAAs, trimethylamine N-oxide, beta-hydroxybutyrate, trimethyl uric acid, 
      and alanine. A targeted MS approach showed significant differences in 
      lysophosphatidylcholines, phosphatidylcholines, acylcarnitine, amino acids and 
      sphingomyelins; Lyso.PC.a.C18.0, PC.ae.C34.2, C3.DC..C4.OH, glutamine and 
      SM.C16.1, being the most significant metabolites. Pearson's correlation studies 
      showed associations between these metabolites and the clinical chemistry 
      parameters across both cohorts. This report identified differences in metabolites 
      in response to T2DM in agreement with many published population studies. This 
      contributes to the global search for a bank of metabolite biomarkers that can 
      predict the advent of T2DM and give insight to its pathogenic mechanisms.
FAU - Fikri, Asma M
AU  - Fikri AM
AD  - Ministry of Health and Prevention, Dubai, UAE. dr.asma.fikri@gmail.com.
AD  - Rahsid Centre for Diabetes and Research, Ajman, UAE. dr.asma.fikri@gmail.com.
FAU - Smyth, Rosemary
AU  - Smyth R
AD  - Department of Pharmaceutical and Biological Chemistry, UCL School of Pharmacy, 
      London, UK.
FAU - Kumar, Vijay
AU  - Kumar V
AD  - Rahsid Centre for Diabetes and Research, Ajman, UAE.
FAU - Al-Abadla, Zainab
AU  - Al-Abadla Z
AD  - Rahsid Centre for Diabetes and Research, Ajman, UAE.
FAU - Abusnana, Salahedeen
AU  - Abusnana S
AD  - Rahsid Centre for Diabetes and Research, Ajman, UAE.
FAU - Munday, Michael R
AU  - Munday MR
AD  - Department of Pharmaceutical and Biological Chemistry, UCL School of Pharmacy, 
      London, UK. michael.munday@ucl.ac.uk.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201019
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Amino Acids, Branched-Chain)
RN  - 0 (Biomarkers)
RN  - 0 (Methylamines)
RN  - 268B43MJ25 (Uric Acid)
RN  - FLD0K1SJ1A (trimethyloxamine)
RN  - OF5P57N2ZX (Alanine)
RN  - TZP1275679 (3-Hydroxybutyric Acid)
SB  - IM
MH  - 3-Hydroxybutyric Acid/blood
MH  - Adult
MH  - Alanine/blood
MH  - Amino Acids, Branched-Chain/blood
MH  - Biomarkers/*blood
MH  - Chromatography, Liquid
MH  - Diabetes Mellitus, Type 2/blood/*diagnosis/etiology
MH  - Female
MH  - Humans
MH  - Male
MH  - Metabolomics
MH  - Methylamines/blood
MH  - Middle Aged
MH  - Obesity/blood/*complications
MH  - Tandem Mass Spectrometry
MH  - United Arab Emirates
MH  - Uric Acid/blood
MH  - Young Adult
PMC - PMC7572402
COIS- The authors declare no competing interests.
EDAT- 2020/10/21 06:00
MHDA- 2021/01/20 06:00
PMCR- 2020/10/19
CRDT- 2020/10/20 06:18
PHST- 2020/02/11 00:00 [received]
PHST- 2020/09/16 00:00 [accepted]
PHST- 2020/10/20 06:18 [entrez]
PHST- 2020/10/21 06:00 [pubmed]
PHST- 2021/01/20 06:00 [medline]
PHST- 2020/10/19 00:00 [pmc-release]
AID - 10.1038/s41598-020-73384-7 [pii]
AID - 73384 [pii]
AID - 10.1038/s41598-020-73384-7 [doi]
PST - epublish
SO  - Sci Rep. 2020 Oct 19;10(1):17616. doi: 10.1038/s41598-020-73384-7.