PMID- 33081162
OWN - NLM
STAT- MEDLINE
DCOM- 20210415
LR  - 20210415
IS  - 2079-6374 (Electronic)
IS  - 2079-6374 (Linking)
VI  - 10
IP  - 10
DP  - 2020 Oct 16
TI  - Fabrication and Characterization of Simple Structure Fluidic-Based Memristor for 
      Immunosensing of NS1 Protein Application.
LID - 10.3390/bios10100143 [doi]
LID - 143
AB  - Non-structural protein 1 (NS1 protein) is becoming a commonplace biomarker for 
      the diagnostic of early detection of dengue. In this study, we sought to use a 
      label-free approach of detecting NS1 protein by harnessing fluidic-based 
      memristor sensor. The sensor was fabricated using sol-gel spin coating technique, 
      by which TiO(2) thin film is coated on the surface of Indium tin oxide (ITO) and 
      a glass substrate. The sensor was then functionalized with 
      glycidoxypropyl-trimethoxysilane (GPTS), acting as antibody for NS1. The addition 
      of the target NS1 formed an antibody-antigen complex which altered the physical 
      and electrical properties in sensing region. Sensing of the sensor is incumbent 
      upon the measurement of Off-On resistance ratio. Imaging with Field Emission 
      Scanning Electron Microscope (FESEM) evinced the successful immobilization of the 
      antibody and the subsequent capture of the NS1 protein by the immobilized 
      antibody. The detection limit actualized by the developed sensor was 52 nM and 
      the diameter of 2 mm gives the most optimal measurement. The developed sensor 
      demonstrated an immense potential towards the development of label-free 
      diagnostic of early dengue infection.
FAU - Mohamad Hadis, Nor Shahanim
AU  - Mohamad Hadis NS
AUID- ORCID: 0000-0001-5517-4276
AD  - Collaborative Microelectronic Design Excellence Centre (CEDEC), Sains@USM, 
      Universiti Sains Malaysia, Pulau Pinang 11900, Malaysia.
AD  - Faculty of Electrical Engineering, Universiti Teknologi MARA Cawangan Pulau 
      Pinang, Kampus Permatang Pauh, Pulau Pinang 13500, Malaysia.
FAU - Manaf, Asrulnizam Abd
AU  - Manaf AA
AUID- ORCID: 0000-0002-6198-263X
AD  - Collaborative Microelectronic Design Excellence Centre (CEDEC), Sains@USM, 
      Universiti Sains Malaysia, Pulau Pinang 11900, Malaysia.
FAU - Rahman, Mohamad Faizal Abd
AU  - Rahman MFA
AD  - Collaborative Microelectronic Design Excellence Centre (CEDEC), Sains@USM, 
      Universiti Sains Malaysia, Pulau Pinang 11900, Malaysia.
AD  - Faculty of Electrical Engineering, Universiti Teknologi MARA Cawangan Pulau 
      Pinang, Kampus Permatang Pauh, Pulau Pinang 13500, Malaysia.
FAU - Ngalim, Siti Hawa
AU  - Ngalim SH
AUID- ORCID: 0000-0001-7893-926X
AD  - Regenerative Medicine Cluster, Advanced Medical & Dental Institute (AMDI), 
      Universiti Sains Malaysia, Pulau Pinang 13200, Malaysia.
FAU - Hock Tang, Thean
AU  - Hock Tang T
AUID- ORCID: 0000-0001-7652-7923
AD  - Infectomics Cluster, Advanced Medical & Dental Institute (AMDI), Universiti Sains 
      Malaysia, Pulau Pinang 13200, Malaysia.
FAU - Citartan, Marimuthu
AU  - Citartan M
AD  - Infectomics Cluster, Advanced Medical & Dental Institute (AMDI), Universiti Sains 
      Malaysia, Pulau Pinang 13200, Malaysia.
FAU - Ismail, Aziah
AU  - Ismail A
AUID- ORCID: 0000-0001-7850-6405
AD  - Institute for Research in Molecular Medicine (INFORMM), Universiti Sains 
      Malaysia, Pulau Pinang 11800, Malaysia.
FAU - Herman, Sukreen Hana
AU  - Herman SH
AUID- ORCID: 0000-0003-1588-557X
AD  - Nano-Electronics Centre (NET), Universiti Teknologi MARA, Shah Alam, Selangor 
      40450, Malaysia.
LA  - eng
GR  - 203/PCEDEC/6071289/Ministry of Higher Education Malaysia under Fundamental 
      Research Grant Scheme/
GR  - 1001/PCEDEC/870050/Top Down Research University (RU) Grant Universiti Sains 
      Malaysia/
GR  - 311/CIPPM/4401005/Higher Institution Center of Excellence (HiCoE)/
PT  - Journal Article
DEP - 20201016
PL  - Switzerland
TA  - Biosensors (Basel)
JT  - Biosensors
JID - 101609191
RN  - 0 (Proteins)
RN  - 0 (Silanes)
RN  - 0 (Tin Compounds)
RN  - 71243-84-0 (indium tin oxide)
RN  - V1J39XPF91 (trimethoxysilane)
SB  - IM
MH  - Biosensing Techniques/*methods
MH  - Proteins
MH  - Silanes
MH  - Tin Compounds
PMC - PMC7602703
OTO - NOTNLM
OT  - NS1 protein
OT  - current-voltage measurement
OT  - fluidic-based memristor sensor
OT  - off-on resistance ratio
COIS- The authors declare no conflict of interest.
EDAT- 2020/10/22 06:00
MHDA- 2021/04/16 06:00
PMCR- 2020/10/01
CRDT- 2020/10/21 01:02
PHST- 2020/09/02 00:00 [received]
PHST- 2020/10/05 00:00 [revised]
PHST- 2020/10/13 00:00 [accepted]
PHST- 2020/10/21 01:02 [entrez]
PHST- 2020/10/22 06:00 [pubmed]
PHST- 2021/04/16 06:00 [medline]
PHST- 2020/10/01 00:00 [pmc-release]
AID - bios10100143 [pii]
AID - biosensors-10-00143 [pii]
AID - 10.3390/bios10100143 [doi]
PST - epublish
SO  - Biosensors (Basel). 2020 Oct 16;10(10):143. doi: 10.3390/bios10100143.